In vitro sensitivity of environmental isolates of pathogenic dematiaceous fungi to azole compounds and a phenylpropyl-morpholine derivative

Okeke, C. N.; Gugnani, H. C.

doi:10.1007/bf00437440

Cited by 16 publications

(6 citation statements)

References 16 publications

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“…The minimal inhibition concentrations (MICs) of the test agents were established using agar dilution method, described by 8 and modified by 9 .…”

Section: Determination Of Minimal Inhibition Concentration (Mics)mentioning

confidence: 99%

Detection of Causative Agents, Antifungal Susceptibility Profile and Cross-Resistance of Candida albicans isolated from oral and Vaginal Candidiasis

Ismaeel Nassir Al-Rubaye,

H.Al-Hamadani,

AL- Hilaly

2017

Qad Med J

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This study was designed to investigate antifungal susceptibility and cross resistance of C. albicans isolates toward four antifungal drugs; Amphotericin B, nystatin, fluconazole and ketoconazole The study groups included 208 patients( 108 women and 100 children) who attented the Al-Suwayra Hospital/ Kut Province during the period from December 2008 to April 2009. Patients groups were represented by women who were complaining of vaginal discharge and itching and children who were complaining of oral thrush and infected with different type of disease include upper and lower respiratory tract infection, gastroenteritis, kala-azar and septicemia. The study showed that the infection percentage of vaginal candidiasis was 35.1% while oral thrush was 70%. The yeast isolates in two groups were C. albicans (76.8%), C. krusei (1.85%), C. tropicalis (5.5%), C. glabrata (11.1%), C. dublinances (0.9%), Trichosporon (1.85%) and Cryptococcus neoformans (1.85%). The activity of certain antifungal agents were tested against C. albicans isolates by using minimal inhibitory concentration (MICs). The results of range of MICs for amphotericin B was ( 8-128Î¼g/ml), ketoconazole (0.5- 4Î¼g/ml), fluconazole (8-64Î¼g/ml), Nystatin (0.03-2Î¼g/ml). The MICs of amphotericin B to the isolate that mutant to Nystatin tended to rise in parallel from (0.5-1Î¼g/ml) to (4-16Î¼g/ml). There was no correlation between MICs of these strains with fluconazole and ketoconazole. In addition, The MICs of ketoconazole to the isolates that mutant to fluconazole tended to rise in parallel from (0.5-2Î¼g/ml) to (1-8Î¼g/ml). There was no correlation between MICs of these strains with Nystatin and amphotericin B MICs. All the MICs values of the mutant strain return to the original values after remove the drug effect except the Nystatin . The MICs value for the isolate mutant to Nystatin dropped from the range (600-1200Î¼g/ml) to (200-800Î¼g/ml) but not returned to the original range (64-128Î¼g/ml).

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“…The minimal inhibition concentrations (MICs) of the test agents were established using agar dilution method, described by 8 and modified by 9 .…”

Section: Determination Of Minimal Inhibition Concentration (Mics)mentioning

confidence: 99%

Detection of Causative Agents, Antifungal Susceptibility Profile and Cross-Resistance of Candida albicans isolated from oral and Vaginal Candidiasis

Ismaeel Nassir Al-Rubaye,

H.Al-Hamadani,

AL- Hilaly

2017

Qad Med J

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“…The MIC corresponds to the lowest concentration of the drug at which there is no macroscopic growth in all three replicate dishes after 7 days [24] in three independent experiments. The MIC corresponds to the lowest concentration of the drug at which there is no macroscopic growth in all three replicate dishes after 7 days [24] in three independent experiments.…”

Section: Susceptibility To Antimycotic Drugsmentioning

confidence: 99%

Effect of sub-MICs of antimycotics on expression of intracellular esterase ofTrichophyton rubrum

2001

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The electrophoretic pattern of the intracellular esterase of the dermatophyte Trichophyton rubrum was altered when this fungus was grown in the presence of subinhibitory concentrations of the antimycotics tioconazole or griseofulvin. All strains (original isolate and antimycotic resistant mutants) presented five clearly visible bands when cultivated on medium containing below-minimum inhibitory concentrations (sub-MICs) of tioconazole or griseofulvin, and only two clearly visible bands when cultivated in medium without antimycotics. No extra bands were detected in the electrophoretic patterns of the extracellular esterase of these fungi (mutants or the original isolate) when cultivated with or without tioconazole or griseofulvin (sub-MIC values). These results suggest that additional forms of esterase are produced inside the cell and may be a nonspecific response to cellular stress, or may participate in cellular detoxification processes in the presence of these antimycotics.

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“…The isolate was tested for sensitivity to clotrimazole following the agar dilution procedure described earlier (Okeke & Gugnani 1987). Plates of assay medium, Emmon's SDA, containing doubling dilutions of clotrimazole (1W 0.025 pg/ml) were inoculated with 0.05 ml saline suspensions of the fungus (10' conidia/ml).…”

Section: N V I T R O D R U G S E N S I T I V I T Y Testmentioning

confidence: 99%

Curvularia clavata as an aetiological agent of human skin infection

Gugnani

Okeke

Sivanesan

1990

Lett Appl Microbiol

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In vitro sensitivity of environmental isolates of pathogenic dematiaceous fungi to azole compounds and a phenylpropyl-morpholine derivative

Cited by 16 publications

References 16 publications

Detection of Causative Agents, Antifungal Susceptibility Profile and Cross-Resistance of Candida albicans isolated from oral and Vaginal Candidiasis

Detection of Causative Agents, Antifungal Susceptibility Profile and Cross-Resistance of Candida albicans isolated from oral and Vaginal Candidiasis

Effect of sub-MICs of antimycotics on expression of intracellular esterase ofTrichophyton rubrum

Curvularia clavata as an aetiological agent of human skin infection

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