In Vitro Selective Growth-Inhibitory Effect of 8-Hydroxyquinoline on Clostridium perfringens versus Bifidobacteria in a Medium Containing Chicken Ileal Digesta

Abstract

Clostridium perfringens-induced necrotic enteritis is generally controlled by antibiotics. However, because of increasing antibiotic resistance, other antibacterial agents are required, preferably ones that do not affect the beneficial intestinal microbiota of the host. This study evaluated the in vitro selective growth-inhibitory effect of 8-hydroxyquinoline (8HQ) on C. perfringens vs. bifidobacteria in a medium containing chicken ileal digesta. Prior to the experiments, the minimum inhibitory concentrations of 8HQ and penicillin G were determined by broth microdilution assay. The minimum inhibitory concentration values of 8HQ for C. perfringens were 16–32 times lower than the values for bifidobacteria. Treatment of autoclaved and non-autoclaved chicken ileal digesta with 8HQ showed a selective anticlostridial effect. After incubation of C. perfringens with autoclaved ileal digesta for 3 h, all 8HQ concentrations tested (32–2048 μg/mL) significantly reduced C. perfringens bacterial count. In contrast, the same treatment had no or only a slight effect on bifidobacteria counts. Unlike 8HQ, penicillin G did not exhibit any selectivity. Similar results were obtained after incubation for 24 h. In non-autoclaved ileal digesta, all 8HQ concentrations tested significantly reduced C. perfringens bacterial counts after incubation for 30 min and 3 h, while no effect was observed on bifidobacteria. These results suggest that 8HQ may serve as a prospective veterinary compound for use against necrotic enteritis in poultry.

In Vitro Selective Growth-Inhibitory Activities of Phytochemicals, Synthetic Phytochemical Analogs, and Antibiotics against Diarrheagenic/Probiotic Bacteria and Cancer/Normal Intestinal Cells

“…The selective antibacterial activity of 8-hydroxyquinoline against diarrheagenic pathogens seems to be enhanced with chlorine halogenation or by the presence of a nitro group and decreased with iodine halogenation and the presence of a sulfo group, as respectively observed for chloroxine, nitroxoline, and ferron in our study. The in vitro selective anticlostridial effect of 8-hydroxyquinoline with increased resistance of bifidobacteria was previously described in studies of Novakova et al [ 10 , 27 , 28 ], Skrivanova et al [ 29 ], and Kim et al [ 30 ]. However, data on its in vitro growth-inhibitory effects against a broader selection of diarrheagenic bacteria are limited.…”

Antioxidant Properties and the Formation of Iron Coordination Complexes of 8-Hydroxyquinoline

“…8-Hydroxyquinoline (oxine, 8-quinolinol, 8HQ) is an alkaloid with a rather simple chemical structure and occurs in plants of families Asteraceae [1] and Euphorbiaceae [2]. Previous studies revealed a very large spectrum of various biological activities, such as phytotoxic [1,3], antibacterial [4,5], antifungal [6], and insecticidal [2] activities for 8HQ and structurally related compounds. In recent decades, however, its potential as a cure for some neurodegenerative diseases such as Alzheimer’s and Parkinson’s diseases raised attention [7,8].…”

Effect of hydroxychloroquine on oxidative/nitrosative status and angiogenesis in endothelial cells under high glucose condition

“…It seems an intricate network of signaling pathways reported to be involved in NO production. 35 - 37 Irrespective of an inevitable role of PI3K/Akt/mTOR axis iNOS expression, several studies showed HCQ blunted the generation of NO by eliminating 20S proteasomal activation and reduction of interferon-β in macrophages and chondrocytes. 38 In accordance with our data, Gómez-Guzmán et al acclaimed that chronic using of HCQ could protect kidney and improve endothelial cell dysfunction by decreasing ROS level in autoimmune disease.…”