In-vitro selection of sugarcane (Saccharum officinarum L.) putative mutant for drought stress

Abstract

In-vitro selection through tissue culture followed by field testing is one of the breeding methods to improve sugarcane varieties with drought-tolerant. This research aimed to obtain putative mutant sugarcane physically mutated using gamma-ray irradiation that passed the in vitro drought selection using PEG-selecting agents. This study was conducted from March to December 2016 using embryogenic calli of PSJT 941, PS 862, and BL varieties. The experimental design was the factorial randomized complete design with two factors, i.e. irradiation dose (0, 5, 10, 15, 20, 25, 30, 35 Gray) and PEG concentration (0, 10, 20%). The irradiated calli selected by PEG media had changed in color from yellowish-white to brownish and blackish and indicated the growth inhibition. The higher the dosage of irradiation and the concentration of PEG, the more calli with growth inhibition. Some putative callus mutants successfully passed in vitro selection using 10 and 20 % PEG, which showed tolerance to drought stress. The appropriate irradiation dose to produce tolerant mutants is 5 – 30 Grays for PS 862 and BL varieties, and 5-25 Grays for PSJT 941. To recognize the mutant response further, it is necessary for in-vivo selection at the greenhouse and in the field.

Improvement of drought tolerance of patchouli through gamma irradiation and in vitro selection

“…PEG concentration of 20% caused a change in callus color from yellowish-white into dark brown and black. These results are in line with the previous research conducted by [2] and [18], the calli grew on media with a concentration of 20% PEG was generally brown and black. According to [14], the color-changing of calli into brown indicates that the calli experienced water limitation due to the addition of PEG in the media.…”

“…The survived calli assumed has a tolerant character to drought stress. Several study results have been done in drought stress tolerance includes for patchouli plant [16][17], sugarcane [18][19], and wheat [20]. This research objective was to obtain drought-tolerant patchouli putative mutant calli through irradiation with gamma-ray and in vitro selection using the selection agent Polyethylene Glycol (PEG).…”

Drought Stress in Sugarcane: In-vitro Mutagenesis and Selection of Polyethylene Glycol (PEG) Tolerant Callus Lines

“…It was viable to choose mutants during the in-vitro selection procedure by using particular selective agents. Similar research was done on sugarcane by Hartati et al [10], who concluded that the right mutation dosage would produce mutants that could withstand drought.…”