In vitro selection of NaCl tolerant callus lines of Cymbopogon martinii (Roxb.) Wats

Patnaik, Jeetendranath; Debata, B. K.

doi:10.1016/s0168-9452(97)04612-8

Cited by 24 publications

(20 citation statements)

References 18 publications

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“…Moreover, the K + /Na + ratio decreased in response to the intensity and the duration of stress. Similar results were previously reported in alfalfa (Chaudhary et al, 1997) and in Cymbopogon martinii (Patnaik and Debata, 1997), in soy (Liu and Van Staden, 2001) and in rice (Sathish et al, 1997). Likewise, Sairam et al (2002) described a similar trend in wheat genotypes in response to long-term salt stress.…”

Section: Cp59-73 Nco310supporting

confidence: 89%

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

Errabii¹,

Gandonou²,

Bouhdid³

et al. 2017

Int. J. Biotechnol. Mol. Biol. Res.

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In this work, the effect of different concentrations of NaCl on calli induced from two sugarcane cultivars NCo310 and CP59-73 was studied. Growth and ion concentrations (Na + , Cl -, K + and Ca 2+ ) of calli were determined after 1, 2 and 3 months of stress with the objective to understand the cellular mechanisms operating in salt stress tolerance and to determine the implication of inorganic fraction in salt tolerance in sugarcane cultivars. A negative effect of the NaCl concentration and the duration of stress exposure on the callus rate growth was observed in both cultivars and with more extent in CP59-73 cv. Results showed an increase in Na + and Cl -and a decrease in K + and Ca 2+ concentrations after 1, 2 and 3 months of salt stress exposure. It also showed that resistant cv. NCo310 stressed calli accumulated less Na + and retained more K + and Ca 2+ than CP59-73 calli. Cl -appeared to be involved in osmotic adjustment since the resistant cv. NCo310 stressed calli accumulated more Cl -than CP59-73 ones. These results suggested that the resistance to salinity in sugarcane is associated with a high K + , Ca 2+ and Cl -concentrations and a low Na + concentration within cells.Key words: sugarcane (Saccharum sp.), salt stress, ion uptake, callus growth, long-term stress exposure. INTRODUCTIONSalinity is a significant factor that affects crop production and agricultural sustainability worldwide, since about 10% of the land surface and 50% of all irrigated land in the world are prone to salinity (Flowers et al., 2010). Salt stress affects several aspects of plant physiology by its osmotic and ionic components (Munns and Tester, 2008 However, the operating mechanisms remains till now poorly understood and it not easy to differentiate between the effects due to the osmotic component and those due to the ionic one (Gandonou et al., 2011). Adaptation to salt stress involves several mechanisms that help plants to adjust osmotically, to maintain a low cytoplasmic concentration of toxic ions and a high concentration of essential minerals (Munns, 2005). It is well known that cell culture techniques, including callus culture constitutes an important tool to investigate the response to salts, such as NaCl, of several plants at cellular level (Perez-Alfocea et al., 1994;Lutts et al., 1996;Ehsanpour and Fatahian, 2003;Gandonou et al., 2011) and therefore, to understand the cellular mechanisms involved in the salt tolerance and/or sensitivity (Tal, 1983;Gandonou et al., 2011). As for sugarcane, there were a few studies on the implication of inorganic solutes to osmotic adjustment and salt tolerance at the cell level. In a previous study, it was shown that ions toxicity was implied in salt effect at cellular level and that K + ion plays a crucial role in sugarcane salt-tolerance (Gandonou et al., 2005a). In the present investigation, the effects of different NaCl concentrations (0, 50, 100 and 150 mM) and the duration of stress exposure on callus growth and ion concentration in two sugarcane genotypes, CP59-73 and NCo310, at t...

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Section: Cp59-73 Nco310supporting

confidence: 89%

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

Errabii¹,

Gandonou²,

Bouhdid³

et al. 2017

Int. J. Biotechnol. Mol. Biol. Res.

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“…Palmarosa callus tissue grown from cultured nodal explants was subjected to a range of NaCl concentrations. A positive correlation was observed between Pro accumulation and salt stress, since high levels of Pro were found in the salt-resistant callus lines (Patnaik and Debata, 1997). The concentrations of soluble carbohydrates and Pro were higher in the saltresistant lines of Glycine max Merr ' Acme' callus cultures (Liu and van Staden, 2000).…”

Section: Salinity Stressmentioning

confidence: 84%

Metabolic and molecular-genetic regulation of proline signaling and itscross-talk with major effectors mediates abiotic stress tolerance in plants

Roychoudhury¹,

Banerjee²,

Lahiri³

2015

Turk J Bot

105

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Proline (Pro) accumulation is a common response of several plant species to combat abiotic stresses. Under stress conditions, Pro acts as an excellent compatible solute in the plant system, participating in the alleviation of stress sensitivity. Though the metabolic pathways associated with Pro are well studied, parts of its regulatory cascades are still not properly known. It has also been conjectured that epigenetic modifications regulate Pro metabolism during abiotic stress. Apart from Pro, the plant abiotic stress responses are essentially mediated by multiple effectors. Hence, proper analysis of the cross-talks of Pro with the other components of the abiotic stress response has turned out to be mandatory in order to design multistress-tolerant transgenic lines. Highlighting the relation between Pro and seed germination is also essential to understand the notion behind plant susceptibility and survival during stress. Generally, Pro has a universal mechanism to generate abiotic stress tolerance through stabilization of structural components, enzyme structures, and regulation of osmotic adjustments. The success achieved through recent transgenic approaches leading to more accumulation of Pro in the sink has also been focused on in the present review.

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“…In several plant species, increasing the concentration of salts such as NaCl in the culture medium resulted in a steady increase in the Na + content in callus cultures (Kumar and Sharma, 1989;Patnaik and Debata, 1997;Unnikrishnan et al, 1991). In date palm, Al-Khayri (2002) reported a significant increase in Na + concentration when the callus cultures were augmented with 25 mM NaCl.…”

Section: Sodium Potassium and Chloride Ions Contentmentioning

confidence: 99%

“…Cell and tissue culture techniques have been successfully employed for screening and developing stress tolerant varieties of various crops (Patnaik and Debata, 1997). The use of in vitro cultures to study abiotic stress responses is based on the fact that in vitro cultured cells behave similarly to cells of intact plants subjected to water deficit and salinity stress conditions (Attree et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

Al-Khayri

Ibraheem²

2014

Emir. J. Food Agric

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The advent of agricultural biotechnology offers new approaches for the genetic improvement of date palm (Phoenix dactylifera L.), an important economic fruit crop in arid regions. In vitro studies were conducted to recover date palm plants exhibiting enhanced tolerance to salinity and drought stress, two major agricultural problems in arid areas. The response of date palm callus cultures to salt stress was investigated by exposing cell suspension and callus cultures to varying concentrations of sodium chloride (NaCl). The reaction of callus to the duration of exposure to NaCl, potassium chloride (KCl) and calcium chloride (CaCl2) was evaluated. Similarly, drought tolerance was assessed in response to varying concentrations of polyethylene glycol (PEG-8000). Significant differences were reported in various parameters measured including callus growth, somatic embryogenesis, proline content and ion distribution. Calli with enhanced tolerance to these selection agents were obtained; however, plant regeneration from these cell lines was not realized. Although the available data provide an insight of the behavior of date palm callus in the presence of salt and drought stress, further research work is required to refine the selection procedures and plant regeneration from the selected tolerant cell lines.

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In vitro selection of NaCl tolerant callus lines of Cymbopogon martinii (Roxb.) Wats

Cited by 24 publications

References 18 publications

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

Callus growth and ion composition in response to long-term NaCl-induced stress in two sugarcane (Saccharum sp.) cultivars

Metabolic and molecular-genetic regulation of proline signaling and itscross-talk with major effectors mediates abiotic stress tolerance in plants

In vitro selection of abiotic stress tolerant date palm (Phoenix dactylifera L.): A review

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