In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detection

Bruno, John G.; Kiel, Johnathan L.

doi:10.1016/s0956-5663(99)00028-7

Cited by 266 publications

(130 citation statements)

References 14 publications

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“…The term 'aptamer' means 'to fit' (aptus) in Latin (6), which indicates two important properties of aptamers: i) their ability to fold into complex tertiary structures and recognize their targets with high affinity (low nM to high pM equilibrium dissociation constants); and ii) their specificity, somewhat analogous to antigen-antibody interactions. Using this technique, a number of aptamers that specifically recognize targets, such as metal ions (11)(12)(13), organic dyes and amino acids (14)(15)(16)(17), antibiotics (18,19) and peptides (20,21), as well as proteins of various sizes and functions (6,22,23), whole cells (24)(25)(26)(27), whole organisms (28), viruses (29) and bacteria (30), have been obtained (31)(32)(33). The SELEX process is based on the ability of these small oligonucleotides to fold into unique 3D structures that interact with a target with high specificity and affinity through such interactions as van der Waals surface contacts, hydrogen bonding and base stacking.…”

Section: Aptamers and Selexmentioning

confidence: 99%

Clinical applications of nucleic acid aptamers in cancer

Pei

Zhang

Liu

2014

Molecular and Clinical Oncology

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Abstract. Nucleic acid aptamers are small single-stranded DNA or RNA oligonucleotide segments, which bind to their targets with high affinity and specificity via unique three-dimensional structures. Aptamers are generated by an iterative in vitro selection process, termed as systematic evolution of ligands by exponential enrichment. Owing to their specificity, non-immunogenicity, non-toxicity, easily modified chemical structure and wide range of targets, aptamers appear to be ideal candidates for various clinical applications (diagnosis or treatment), such as cell detection, target diagnosis, molecular imaging and drug delivery. Several aptamers have entered the clinical pipeline for applications in diseases such as macular degeneration, coronary artery bypass graft surgery and various types of cancer. The aim of this review was to summarize and highlight the clinical applications of aptamers in cancer diagnosis and treatment.

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Section: Aptamers and Selexmentioning

confidence: 99%

Clinical applications of nucleic acid aptamers in cancer

Pei

Zhang

Liu

2014

Molecular and Clinical Oncology

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“…[9] The authors utilized centrifugation for partitioning the unbound DNA sequences from the DNA-spore complexes. Bruno and Kiel used both an aptamer-magnetic bead-electrochemiluminescence sandwich assay and a colorimetric assay to demonstrate binding to the anthrax spore target and established a wide dynamic range from <10->6 x 10 6 anthrax spores.…”

Section: Bacillus Anthracis Spores (Cat-a Pathogen)mentioning

confidence: 99%

Aptasensors for biosecurity applications

Fischer

Tarasow

Tok

2007

Current Opinion in Chemical Biology

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SUMMARY:Nucleic acid aptamers have found steadily increased utility and application steadily over the last decade. In particular, aptamers have been touted as a valuable complement to and in some cases replacement for antibodies due to their structural and functional robustness as well as their ease in generation and synthesis. They are thus attractive for biosecurity applications, e.g. pathogen detection, and are especially well suited since their in vitro generation process does not require infection of any host systems. Herein we provide a brief overview of the aptamers generated against biopathogens over the last few years. In addition, a few recently described detection platforms using aptamers (aptasensors) and potentially suitable for biosecurity applications will be discussed.

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“…The SELEX technique has been used to isolate high affinity aptamers for a wide range of targets, including small organic molecules (13,14), peptides (15,16), soluble proteins such as growth factors, neuropeptides, (17)(18)(19)(20)(21)(22), cell surface epitopes (23), proteins exposed in their membrane environment (24), red blood cell membranes (25), and whole organisms such as parasites (26,27) and anthrax spores (28). Aptamers with possible future therapeutic importance include aptamers that target pathogens such as the hepatitis C virus (29), the HIV virus (30,31), and African and American trypanosomes (26,27).…”

Section: The Selex Techniquementioning

confidence: 99%

RNA and DNA aptamers in cytomics analysis

2004

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BackgroundThe systematic evolution of ligands by exponential enrichment (SELEX) technique is a combinatorial library approach in which DNA or RNA molecules (aptamers) are selected by their ability to bind their protein targets with high affinity and specificity, comparable to that of monoclonal antibodies. In contrast to antibodies conventionally selected in animals, aptamers are generated by an in vitro selection process, and can be directed against almost every target, including antigens like toxins or nonimmunogenic targets, against which conventional antibodies cannot be raised.MethodsAptamers are ideal candidates for cytomics, as they can be attached to fluorescent reporters or nanoparticles in order to study biological function by fluorescence microscopy, by flow cytometry, or to quantify the concentration of their target in biological fluids or cells using ELISA, RIA, and Western blot assays.ResultsWe demonstrate the in vitro selection of anti‐kinin B1 receptor aptamers that could be used to determine B1 receptor expression during inflammation processes. These aptamers specifically recognize their target in a Northern‐Western blot assay, and bind to their target protein whenever they are exposed in the membrane.ConclusionsCurrently, aptamers are linked to fluorescent reporters. We discuss here the present status and future directions concerning the use of the SELEX technique in cytomics. © 2004 Wiley‐Liss, Inc.

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In vitro selection of DNA aptamers to anthrax spores with electrochemiluminescence detection

Cited by 266 publications

References 14 publications

Clinical applications of nucleic acid aptamers in cancer

Clinical applications of nucleic acid aptamers in cancer

Aptasensors for biosecurity applications

RNA and DNA aptamers in cytomics analysis

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