In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection

Tang, Jijun; Yu, Tao; Guo, Lei; Xie, Jianwei; Shao, Ningsheng; He, Zhike

doi:10.1016/j.bios.2006.09.015

Cited by 102 publications

(78 citation statements)

References 22 publications

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“…Aptamers are superior to antibodies because of their broader range of application. They are able to bind not only to proteins (Tang et al 2007;Hasegawa et al 2008;Tsukakoshi et al 2010) but also to other molecules, including metal ions (Kawakami et al 2000), and small molecules (Mann et al 2005;Niazi et al 2008;Cruz-Aguado and Penner 2008;Kim et al 2010;Mehta et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Selection of DNA aptamers that bind to four organophosphorus pesticides

et al. 2012

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Single-stranded DNA (ssDNA) aptamers against four organophosphorus pesticides (phorate, profenofos, isocarbophos and omethoate) were simultaneously isolated from an immobilized random ssDNA library by systematic evolution of ligands by exponential enrichment (SELEX) technique. After 12 rounds of in vitro selection, five ssDNA aptamer candidates were selected and their binding affinities were identified by a novel method using a molecular beacon. Two of the five ssDNA sequences, SS2-55 and SS4-54, demonstrated higher affinities and specificities to the four organophosphorus pesticides. They were defined as broad-spectrum aptamers binding to four different targets and their simulated secondary structures showed highly distinct features with typical stem and loop structures. The dissociation constant of SS2-55 and SS4-54 binding to the four organophosphorus pesticides ranged from 0.8 to 2.5 μM. These aptamers offered application potential in the analysis and/or neutralization of the residues of the four organophosphorus pesticides.

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Section: Introductionmentioning

confidence: 99%

Selection of DNA aptamers that bind to four organophosphorus pesticides

et al. 2012

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“…Several aptamers have been developed for certain biological threat agents (e.g. ricin [3] and ricin analog [4] and anthrax spores [5] ), cancer biomarkers, [6 -9] foodborne pathogens, [10] and other biologically important biomolecules, such as HIV-1 Tat protein, [11,12] cytokines, [13] immunoglobulin E (IgE), [14,15] and thrombin. [16] They are also able to bind to metal ions and small molecules, making them useful in the detection and analysis of environmental pollutants (e.g.…”

Section: Introductionmentioning

confidence: 99%

Raman and surface‐enhanced Raman spectroscopic studies of the 15‐mer DNA thrombin‐binding aptamer

Pagba

Lane

Wachsmann‐Hogiu

2009

J Raman Spectroscopy

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Aptamers are single-stranded oligonucleotides that selectively bind to their target molecules owing to their ability to form secondary structures and shapes. The 15-mer (5 -GGTTGGTGTGGTTGG-3 ) DNA thrombin-binding aptamer (TBA) binds to thrombin following the formation of a quadruplex structure via the Hoogsten-type G-G interactions. In the present study, Raman and SERS spectra of TBA and thiolated TBA (used to facilitate covalent bonding to metal nanoparticle) in different conditions are investigated. The spectra of the two analogs exhibit vibrations, such as the C8 N7-H2 deformation band at ∼1480 cm −1 of the guanine tetrad, that are characteristic of the quadruplex structure in the presence of K + ions or at low temperature. Interestingly, SERS spectra of the two analogs differ markedly from their respective normal Raman spectra, possibly due to changes in the conformation of the aptamer upon binding, as well as to the specific interaction of individual vibrational modes with the metal surface. In addition, the SERS spectra of the thiolated aptamer show significant changes with different concentrations, which may be due to different orientation of the molecule with respect to the metal surface. This study provides useful information for the development of label-free aptamer-based SERS sensors and assays.

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“…Similarly, by aptamer-based surface-enhanced Raman scattering technique, He et al [72] detected 50-100 ng/mL of ricin in liquid foods. Tang et al [73] established a highly selective and sensitive aptamer-based abrin assay by using a molecular light switching reagent [Ru(phen) 2 (dppz)] 2+ . The detection limit was 1 nM in the linear scope from 1 to 400 nM.…”

Section: Biotoxinsmentioning

confidence: 99%

“…In this paper, after briefly describing the screening technique of aptamers, we focus on the applications of aptamers in food safety, and future prospectives are also discussed. Basic principle References CE-SELEX Selection efficiency is promoted and the selection course is decreased (two to four rounds), but the number of DNA in the initial library is limited because of the small sample injection volume (nL level) [73] Toggling SELEX Ability to obtain the species crossing reactive aptamers during alternating rounds of selection; the functional activity of aptamers is promoted [4] Tailored SELEX Truncation experiments are not needed and mainly use for selection of short aptamers [4] Whole-cell SELEX Large range of screening target molecules from original single target molecules to composite target molecules; use of the same random library to select aptamers against multiple target molecules in animal cells or bacterial pathogens at the same time, shortened the screening cycle; enable to select aptamers with high affinity and specificity [4] FluMag-SELEX Easy handing; use of very small amounts of target molecules for aptamer selection; rapid and efficient separation of bound and unbound molecules; strict washing steps; avoid of radioactive labeling [38,39,43] Primer-free genomic SELEX…”

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Technology for Food Analysis

Liu

Zhang

2014

Appl Biochem Biotechnol

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Aptamers are short and functional single-stranded oligonucleotide sequences selected from systematic evolution of ligands by exponential enrichment (SELEX) process, which have the capacity to recognize various classes of target molecules with high affinity and specificity. Various analytical aptamers acquired by SELEX are widely used in many research fields, such as medicine, biology, and chemistry. However, the application of this innovative and emerging technology to food safety is just in infant stage. Food safety plays a very important role in our daily lives because varieties of poisonous and harmful substances in food affect human health. Aptamer technique is promising, which can overcome many disadvantages of existing detection methods in food safety, such as long detection time, low sensitivity, difficult, and expensive antibody preparation. This review provides an overview of various aptamer screening technologies and summarizes the recent applications of aptamers in food safety, and future prospects are also discussed.

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In vitro selection of DNA aptamer against abrin toxin and aptamer-based abrin direct detection

Cited by 102 publications

References 22 publications

Selection of DNA aptamers that bind to four organophosphorus pesticides

Selection of DNA aptamers that bind to four organophosphorus pesticides

Raman and surface‐enhanced Raman spectroscopic studies of the 15‐mer DNA thrombin‐binding aptamer

Aptamer-Based Technology for Food Analysis

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