2001
DOI: 10.1006/jmbi.2001.4981
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In vitro selection of allosteric ribozymes: theory and experimental validation11Edited by R. Huber

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Cited by 47 publications
(23 citation statements)
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“…20-23 However, such sequence alterations usually undermine the affinity and specificity of the molecule, and a strategy for directly selecting for the desired function may therefore prove more advantageous. Toward this end, pioneering work from the Ellington, 24,25 Breaker, 26,27 and Famulok groups 28,29 has demonstrated the selection of allosteric ribozymes that exhibit enhanced catalytic activity in response to binding of various ligands. Moreover, there have been several successes in isolating allosterically-regulated aptamers that can fluorescently report binding events.…”
mentioning
confidence: 99%
“…20-23 However, such sequence alterations usually undermine the affinity and specificity of the molecule, and a strategy for directly selecting for the desired function may therefore prove more advantageous. Toward this end, pioneering work from the Ellington, 24,25 Breaker, 26,27 and Famulok groups 28,29 has demonstrated the selection of allosteric ribozymes that exhibit enhanced catalytic activity in response to binding of various ligands. Moreover, there have been several successes in isolating allosterically-regulated aptamers that can fluorescently report binding events.…”
mentioning
confidence: 99%
“…In this approach, an entire allosteric domain of the ribozyme is randomized and an allosteric selection protocol is applied to isolate constructs whose cleavage activity is activated or deactivated in the presence of external effectors [50][51][52]. A protein dependent RNA-ligase ribozyme was also reported using an allosteric selection technique [53,54].…”
Section: Generation Of Allosteric Ribozymesmentioning
confidence: 99%
“…Two main methods exist for distinguishing and recovering these molecules. The fi rst, selection by auto-modifi cation, is used with nucleic acids that posses self-processing (3,4,7,8,9,12,17,18,35,50,51,81,90) or ligase activity (5,20,26,40,96); these can be discriminated by their size. When selecting molecules with autophosphorylation (71) or acylation activity (44), active molecules can be recovered by affi nity chromatography.…”
Section: Theoretical Basis Of In Vitro Selectionmentioning
confidence: 99%