In vitro Selection of a Peptide with High Selectivity for Cardiomyocytes In vivo

McGuire, Michael J.; Samli, Kausar N.; Johnston, Stephen Albert; Brown, Kathlynn C.

doi:10.1016/j.jmb.2004.06.029

Cited by 74 publications

(83 citation statements)

References 31 publications

(20 reference statements)

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“…Whereas some diffuse staining is observed in the liver of rats infused with RIP1, similar staining is seen with the control phage containing a random peptide sequence (online appendix) consistent with nonspecific clearance of the phage by the reticuloendothelial system. Similar results have been observed by our laboratory (15) and others (9,11). Interestingly, animals infused with the RIP1 phage exhibit significant staining in the kidney tubules.…”

Section: ϫ5supporting

confidence: 90%

“…Sequence determination. Random phage clones from titration plates were routinely sequenced after round 3 of the library panning to identify convergent peptide sequences as previously described (15). Convergent peptide-displaying phage clones were isolated for further characterization.…”

Section: Methodsmentioning

confidence: 99%

“…Spleen samples were also blocked using FC-Block (1:200) (BD Pharmingen, San Diego, CA) to minimize non-antigenspecific binding. The samples were incubated overnight at 4°C with a 1:50 dilution of mouse anti-phage polyclonal antibody (15) or mouse monoclonal anti-insulin antibody (Sigma, St. Louis, MO) in PBS ϩ /0.5% BSA. Excess antibody was washed off with PBS ϩ containing 0.5% BSA and a 1:24 dilution of fluorescein isothiocyanate-conjugated goat anti-mouse immunoglobulin antibody (Jackson ImmunoResearch Laboratories) was incubated with the tissues for 1 h at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…Targeting to islets is made challenging by the fact that these cells in aggregate represent only 2% by weight of the entire pancreas (12). We have previously demonstrated that phage panning can be carried out on cells in culture to obtain peptides that mediate specific binding to the target cells while not binding to undesired cell types (8,(13)(14)(15)(16)(17). The high discriminating power of the selected phage suggests that peptides could be identified that specifically bind to ␤-cells within the pancreas.…”

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confidence: 99%

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Peptide-Mediated Targeting of the Islets of Langerhans

et al. 2005

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Strategies for restoring ␤-cell function in diabetic patients would be greatly aided by the ability to target genes, proteins, or small molecules specifically to these cells. Furthermore, the ability to direct imaging agents specifically to ␤-cells would facilitate diagnosis and monitoring of disease progression. To isolate ligands that can home to ␤-cells in vivo, we have panned a random phage-displayed 20-mer peptide library on freshly isolated rat islets. We have isolated two 20-mer peptides that bind to islets ex vivo. One of these peptides preferentially homes to the islets of Langerhans in a normal rat with clear differentiation between the endocrine and exocrine cells of the pancreas. Furthermore, this peptide does not target ␤-cells in a type 2 diabetes animal model, suggesting that the peptide can discriminate between glucose-stimulated insulin secretion-functional and -dysfunctional ␤-cells. Diabetes 54: 2103-2108, 2005

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Section: ϫ5supporting

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Peptide-Mediated Targeting of the Islets of Langerhans

et al. 2005

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“…Phage display assists in identifying peptides that target a specifi c receptor, or certain cell types even if the receptors are unidentifi ed [ 49 ]. Till date, various types of receptors or cells, have been discovered such as integrin receptors [ 50 , 51 ], thrombin receptors [ 52 ], tumor cells [ 53 -55 ], cardiomyocytes [ 56 ], and pancreatic β cells [ 57 ]. Tumor-targeting peptides have been effectively used in delivery vehicles for targeting small molecule drugs, oligonucleotides, liposomes, imaging agents, and inorganic nanoparticles to tumors.…”

Section: Protein and Peptide Based Carrier Systemsmentioning

confidence: 99%

Prodrug Conjugate Strategies in Targeted Anticancer Drug Delivery Systems

Banerjee¹,

Todkar²,

Chate

et al. 2014

Advances in Delivery Science and Technology

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Multifunctional Polymeric Nanostructures for Therapy and Diagnosis

Contreras-García¹,

Bucio²

2013

Nanomaterials in Drug Delivery, Imaging, and Tissue Engineering

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By definition, nanomaterials are multifunctional due to the co-incorporation of both therapeutic and bioimaging agents. Among the therapeutic applications of the polymeric nanomaterials are found drug, protein or gene delivery, tissue engineering. Other advances in bioapplications include cell-labeling, cell membrane modeling, agent delivery and targeting. Additionally, imaging nanobiotechnology is applied to sensorization and diagnosis with biosensors and biomarkers for biodetection and bioimaging, respectively. In this chapter are described the different polymeric nanostructures applied to therapy and diagnosis such as polymeric-based core-shell colloid, proteins and peptides, drug conjugates and complexes with synthetic polymers, dendrimers, vesicles, micelles, carbon nanotubes, biopolymers, smart nano polymers, metal-polymer complexes, enzyme-responsive nanoparticles, and hybrid polymeric nanomaterials. The different types of polymers are fabricated using several approaches ranging from conventional chemical methods to more sophisticated processes such as surface modification using ionizing radiation to obtain layers free of pollutants without purification and isolation processes among other advantages.

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In vitro Selection of a Peptide with High Selectivity for Cardiomyocytes In vivo

Cited by 74 publications

References 31 publications

Peptide-Mediated Targeting of the Islets of Langerhans

Peptide-Mediated Targeting of the Islets of Langerhans

Prodrug Conjugate Strategies in Targeted Anticancer Drug Delivery Systems

Multifunctional Polymeric Nanostructures for Therapy and Diagnosis

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