In vitro selection of a photoresponsive RNA aptamer to hemin

Liu, Mingzhe; Jinmei, Hiroshi; Abe, Hiroshi; Ito, Yoshihiro

doi:10.1016/j.bmcl.2010.02.109

Cited by 30 publications

(39 citation statements)

References 30 publications

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“…Azobenzene was also introduced through attachment to the amino group of adenine, and the base pairing was maintained so that in vitro selection involving azobenzene groups became possible (Structure 4 in Figure 3). [22] Mori et al introduced the azobenzene residue into the 5-position of cytosine through an ethynyl group for photoregulation of DNA or RNA hybridization. [23] 4'-Carboxy-4-dimethylaminoazobenzene (Dabcyl) has been widely used as a universal quencher in a molecular beacon, which is a fluorogenic hairpin oligonucleotide probe.…”

Section: Modes Of Nucleic Acid Modification By Azobenzene Derivativesmentioning

confidence: 99%

“…Several photoresponsive aptamers were obtained by in vitro selection or direct molecular design. [22,62] Azobenzenemodified DNA was used to control thrombin activity by changing the structure of the thrombin aptamer. [62a] In the trans form, the azobenzene-modified part hybridizes with part of the aptamer so that it cannot form the correct structure to bind to thrombin.…”

Section: Photoresponsive Nanostructures or Nanodevicesmentioning

confidence: 99%

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Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Li¹,

Wang²,

Liang³

2014

Chemistry An Asian Journal

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Azobenzene has been widely used as a photoregulator due to its reversible photoisomerization, large structural change between E and Z isomers, high photoisomerization yield, and high chemical stability. On the other hand, some azobenzene derivatives can be used as universal quenchers for many fluorophores. Nucleic acid is a good candidate to be modified because it is not only the template of gene expression but also widely used for building well-organized nanostructures and nanodevices. Because the size and polarity distribution of the azobenzene molecule is similar to a nucleobase pair, the introduction of azobenzene into nucleic acids has been shown to be an ingenious molecular design for constructing light-switching biosystems or light-driven nanomachines. Here we review recent advances in azobenzene-modified nucleic acids and their applications for artificial regulation of gene expression and enzymatic reactions, construction of photoresponsive nanostructures and nanodevices, molecular beacons, as well as obtaining structural information using the introduced azobenzene as an internal probe. In particular, nucleic acids bearing multiple azobenzenes can be used as a novel artificial nanomaterial with merits of high sequence specificity, regular duplex structure, and high photoregulation efficiency. The combination of functional groups with biomolecules may further advance the development of chemical biotechnology and biomolecular engineering.

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Section: Modes Of Nucleic Acid Modification By Azobenzene Derivativesmentioning

confidence: 99%

Section: Photoresponsive Nanostructures or Nanodevicesmentioning

confidence: 99%

Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Li¹,

Wang²,

Liang³

2014

Chemistry An Asian Journal

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“…Dieses Aptamer bildet in seiner aktiven Form ein G-Quadruplex und inhibiert die Thrombin-vermittelte Koagulation durch Binden an dessen Exosite 1. [338] Mit der Isolierung einer Hämin-bindenden Aptamersequenz wiesen sie nach, dass Verbindung 29 ein geeignetes Substrat für die T7-RNA-Polymerase darstellt und die resultierende RNA auch als Templat für reverse Transkription dienen kann. [336] In trans hybridisierte die komplementäre Domäne mit der Aptamersequenz, wodurch die G-Quadruplex-Struktur und somit die Funktion des Aptamers zerstçrt wurde.…”

Section: Photoschaltbare Oligonukleotide Und Nukleinsäurenunclassified

Lichtgesteuerte Werkzeuge

Brieke

Rohrbach

Gottschalk³

et al. 2012

Angewandte Chemie

249

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Die zeitliche und räumliche Kontrolle über chemische und biologische Prozesse spielt eine Schlüsselrolle im Leben, wo das Ganze oft viel mehr ist als die Summe seiner Einzelteile. Trivialerweise bilden die Moleküle einer Zelle noch lange kein lebendiges System aus, wenn sie lediglich willkürlich angeordnet werden. Wenn wir diese Zusammenhänge und die Probleme, die durch Fehlfunktionen entstehen, verstehen wollen, benötigen wir Werkzeuge, mit denen wir komplexe Experimente für diese Fragestellungen entwickeln können. Externe Triggersignale, mit denen wir präzise bestimmen können, wo, wann und in welchem Ausmaß ein Prozess gestartet oder gestoppt wird, sind dafür äußerst wertvoll. Licht ist solch ein ideales Triggersignal: Es ist hoch selektiv und bei richtiger Anwendung unschädlich. Es kann mit gut etablierten Techniken erzeugt und manipuliert werden, und viele Ansätze existieren, um Licht in lebenden Systemen anzuwenden – von Zellen bis zu höheren Organismen. Dieser Aufsatz wird sich mit den aktuellen Entwicklungen der letzten sechs Jahre befassen und die zugrundeliegenden Technologien sowie ihre Anwendungen diskutieren.

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“…Although some bi-functional peptides incorporating recognition and signal amplification functions have been reported for analytical applications, 5,6 some additional operations such as cleavage of peptide or release of probe processes were required. [20][21][22] However, peptide aptamers showing peroxidase activity have not been previously reported. Peroxidases are widely used as biocatalysts for bioreactors and biosensors 7 or for conjugation with antibodies.…”

Section: Introductionmentioning

confidence: 99%

A dual functional peptide carrying in vitro selected catalytic and binding activities

et al. 2015

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When minimal functional sequences are used, it is possible to integrate multiple functions on a single peptide chain, like a "single stroke drawing". Here a dual functional peptide was designed by combining in vitro selected catalytic and binding activities. For catalytic activity, we performed in vitro selection for a peptide aptamer binding to hemin by using ribosome display and isolated a peptide that had peroxidase activity in the presence of hemin. By combining the selected catalytic peptide with a peptide antigen, which can be recognized by an antibody, an enzyme-antibody conjugate-like peptide was obtained. This study demonstrates a successful strategy to create dual functionalized peptide chains for use in immunoassays.

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In vitro selection of a photoresponsive RNA aptamer to hemin

Cited by 30 publications

References 30 publications

Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Lichtgesteuerte Werkzeuge

A dual functional peptide carrying in vitro selected catalytic and binding activities

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