In vitro Selection for Resistance against Charcoal Rot Disease of Soybean [Glycine max (L.) Merrill] Caused by Macrophomina phaseolina (Tassi) Goid

Abstract

Background: Soybean is an important oilseed crop. Charcoal rot caused by soil borne polyphagous fungus Macrophomina phaseolina in soybean causes about 80 percent yield loss. Methods: Callus and cell suspension cultures derived from immature and mature embryonic axis and cotyledons explants from disease susceptible soybean cultivars (cv JS335 and JS95-60) were treated with purified toxic culture filtrate generated by the fungus Macrophomina phaseolina fortified with MS culture medium. A continuous method of four cycles of selection was executed on toxic medium while during discontinuous method, a silence was given after the second and third cycle of selection using non-toxic medium. Result: The discontinuous method appeared to be superior as it permitted the calli to recover their regeneration capability. Continuous exposure to toxic culture filtrate resulted up to about 65-75 percent mortality. A total of four lines of JS335 and nine of JS95-60 were found resistant amongst an array of putative resistant/tolerant lines during S1 generation.

Optimization of Different Factors for Initiation of Somatic Embryogenesis in Suspension Cultures in Sandalwood (Santalum album L.)

“…Cell suspension culture may be used to select valuable mutant cell lines through in vitro selection [28][29][30][31] for useful secondary metabolites [32][33][34]. It could also be employed for genetic transformation.…”

Genetic Components and Diversity Analysis in Indian Mustard [Brassica juncea (Linn.) Czern & Coss] Based on Different Morpho-physiological Traits

“…The preference of genetically assorted parents for hybridization progrms is an imperative feature of any crop improvement program to accomplish enviable sergeants [46][47][48][49][50][51]. Since the identification of morphological data-based variation is important for effective evaluation and utilization of genotypes in a breeding program, screening is the only valid way to develop new genotypes for cultivation [46,[52][53][54]. It is demonstrated that the mustard genotypes used in this study have high genetic variability which makes them potential genotypes to foster new cultivars through future mustard breeding.…”

In Vitro Production of Somaclones with Decreased Erucic Acid Content in Indian Mustard [Brassica juncea (Linn.) Czern&Coss]

“…Nevertheless, except for a few, the contribution of in vitro selection in conjunction with somaclonal variation procedures in the breeding program in Brassica juncea is narrow [16], especially for erucic acid. Nonetheless, encouraging reports have been evidenced in wheat and barley [21], soybean [22][23][24][25], groundnut [26][27][28], onion [29], and Withania somnifera [30] with variable degrees of successes.…”