In vitro selection and analysis of RNA aptamer recognize arginine-rich motif (ARM) model peptide on a QCM

Fukusho, Shinobu; Furusawa, Hiroyuki; Okahata, Yoshio

doi:10.1093/nass/44.1.187

Cited by 8 publications

(3 citation statements)

References 5 publications

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“…The SELEX technique has been used to isolate high affinity aptamers for a wide range of targets, including small organic molecules (13,14), peptides (15,16), soluble proteins such as growth factors, neuropeptides, (17)(18)(19)(20)(21)(22), cell surface epitopes (23), proteins exposed in their membrane environment (24), red blood cell membranes (25), and whole organisms such as parasites (26,27) and anthrax spores (28). Aptamers with possible future therapeutic importance include aptamers that target pathogens such as the hepatitis C virus (29), the HIV virus (30,31), and African and American trypanosomes (26,27).…”

Section: The Selex Techniquementioning

confidence: 99%

RNA and DNA aptamers in cytomics analysis

2004

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BackgroundThe systematic evolution of ligands by exponential enrichment (SELEX) technique is a combinatorial library approach in which DNA or RNA molecules (aptamers) are selected by their ability to bind their protein targets with high affinity and specificity, comparable to that of monoclonal antibodies. In contrast to antibodies conventionally selected in animals, aptamers are generated by an in vitro selection process, and can be directed against almost every target, including antigens like toxins or nonimmunogenic targets, against which conventional antibodies cannot be raised.MethodsAptamers are ideal candidates for cytomics, as they can be attached to fluorescent reporters or nanoparticles in order to study biological function by fluorescence microscopy, by flow cytometry, or to quantify the concentration of their target in biological fluids or cells using ELISA, RIA, and Western blot assays.ResultsWe demonstrate the in vitro selection of anti‐kinin B1 receptor aptamers that could be used to determine B1 receptor expression during inflammation processes. These aptamers specifically recognize their target in a Northern‐Western blot assay, and bind to their target protein whenever they are exposed in the membrane.ConclusionsCurrently, aptamers are linked to fluorescent reporters. We discuss here the present status and future directions concerning the use of the SELEX technique in cytomics. © 2004 Wiley‐Liss, Inc.

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Section: The Selex Techniquementioning

confidence: 99%

RNA and DNA aptamers in cytomics analysis

2004

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“…Chlortetracycline (CTC), a broad spectrum antibiotic belongs to the family of tetracyclines (TCs) that possess broad antimicrobial activity against Gram-positive and Gram-negative bacteria, has been widely used as the drug of infectious diseases in the treatment and therapy of humans and as a feed additive in animal growth. − However, constant and extensive applications of TCs, including tetracycline (TC), chlortetracycline (CTC), doxycycline (DOX), oxytetracycline (OTC), and minocycline hydrochloride (MOC), have resulted in antibiotic residues in our food products, such as milk, − eggs, meat, and honey, which may cause serious side effects to some hypersensitive individuals . Among the above TCs, CTC is a semisynthetic tetracycline antibiotic derived from OTC, showing a relatively long half-life in animal tissues, which raised the concerns of CTC residue in food.…”

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Metal–Organic Framework Enhances Aggregation-Induced Fluorescence of Chlortetracycline and the Application for Detection

et al. 2019

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The development of an analytical method for selective and sensitive detection of chlortetracycline (CTC), an often overused broad spectrum antibiotic, is important and challenging in environmental and health monitoring. This paper reports a zinc based metal–organic framework of pyromellitic acid (Zn-BTEC), which has been found to greatly enhance the aggregation-induced emission (AIE) of chlortetracycline. The unique emission response of CTC on Zn-BTEC has been extensively examined and applied for the sensitive detection of CTC on the basis of fluorescence intensity of AIE, and a limit of detection (LOD) was estimated to be 28 nM. A rational mechanism has been proposed based on the porous structure of Zn-BTEC, and the CTC molecules would defuse into the rigid MOF structure and assemble or aggregate, leading to fluorescence enhancement of CTC. Interestingly, the Zn-BTEC materials could discriminate CTC from other TC antibiotics with high selectivity. We have further demonstrated that the Zn-BTEC materials are successfully applied for the sensitive and selective determination of CTC in real samples of fish and urine.

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“…Tetracyclines (TCs) are a group of broad-spectrum polyketide antibiotics, which have a naphthalene ring and three acidic groups: tricarbonyl, phenolic β-diketone, and dimethylamine (Figure ). , The TCs especially oxytetracycline (OTC), doxycycline (DOX), and tetracycline (TET) are extensively used as a veterinary antibiotic and an animal growth promoter. For this reason, they always accumulate in our food products, such as meat, , milk, and eggs, and cause serious side effects to human health. Among the above TCs, OTC is the most frequently used antibiotic because of its effective antimicrobial properties.…”

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Aptamer-Based Cantilever Array Sensors for Oxytetracycline Detection

et al. 2013

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We present a new method for specific detection of oxytetracycline (OTC) at nanomolar concentrations based on a microfabricated cantilever array. The sensing cantilevers in the array are functionalized with self-assembled monolayers (SAMs) of OTC-specific aptamer, which acts as a recognition molecule for OTC. While the reference cantilevers in the array are functionalized with 6-mercapto-1-hexanol SAMs to eliminate the influence of environmental disturbances. The cantilever sensor shows a good linear relationship between the deflection amplitude and the OTC concentration in the range of 1.0-100 nM. The detection limit of the cantilever array sensor is as low as 0.2 nM, which is comparable to some traditional methods. Other antibiotics such as doxycycline and tetracycline do not cause significant deflection of the cantilevers. It is demonstrated that the cantilever array sensors can be used as a powerful tool to detect drugs with high sensitivity and selectivity.

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In vitro selection and analysis of RNA aptamer recognize arginine-rich motif (ARM) model peptide on a QCM

Cited by 8 publications

References 5 publications

RNA and DNA aptamers in cytomics analysis

RNA and DNA aptamers in cytomics analysis

Metal–Organic Framework Enhances Aggregation-Induced Fluorescence of Chlortetracycline and the Application for Detection

Aptamer-Based Cantilever Array Sensors for Oxytetracycline Detection

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