In Vitro Seed and Clonal Propagation of the Mediterranean Aromatic and Medicinal Plant Teucrium capitatum

Papafotiou, Μ.; Martini, Α.Ν.

doi:10.21273/hortsci.51.4.403

Cited by 15 publications

(27 citation statements)

References 52 publications

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“…Microshoots rooted at equally high percentages at IBA concentrations from 0.0 to 4.0 mg • L -1 , in agreement with reports of L. thiniense (Lled o et al, 1996) and L. cavanillesii (Amo-Marco and Ibañez, 1998) but in contrast to our initial results that showed higher rooting percentages in rooting media supplemented with IBA compared with the hormone-free medium (Martini and Papafotiou, 2016). However, this discordance in our results could be attributed to the improvement of rooting capacity of microshoots as subcultures progressed, which has been reported for another Mediterranean xerophyte Teucrium capitatum L. (Papafotiou and Martini, 2016) as well as for various fruit trees (Al-Maarri et al, 1994;Hou et al, 2010;Noiton et al, 1992). The increase in the IBA concentration in the medium led to an analogous increase in callus formation at the base of microshoots, as observed in P. zeylanica (Sahoo and Debata, 1998;Selvakumar et al, 2001).…”

Section: Discussionsupporting

confidence: 44%

In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte Limoniastrum monopetalum

Martini¹,

Papafotiou²

2020

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Limoniastrum monopetalum is an evergreen perennial shrub native to Mediterranean coastal sands and salt marshes. It has adapted to a variety of environmental stresses and is used in traditional medicine and as an ornamental plant. In the present study, an efficient micropropagation protocol for this species was developed to facilitate the production of selected genotypes and promote its wider use. Research has focused on the effects of various cytokinin types [benzyladenine (BA), zeatin, 6-furfurylaminopurine (kinetin) or 6-γ-γ-dimethylallilopurine (2iP)] and concentrations (0.0–4.0 mg·L−1) and various NaCl concentrations (0.0–20 g·L−1) during all stages of in vitro culture. For in vitro establishment, Murashige and Skoog (MS) medium supplemented with 0.5 mg·L−1 BA and 0.0 or 5.0 g·L−1 NaCl was most appropriate (100% explant response, 3–4 shoots per explant, 2 cm shoot length). The best results for shoot multiplication (100% response, 9 shoots per explant, 0.8–1.0 cm shoot length) were obtained with low (0.5 mg·L−1) BA or relatively high (2.0 mg·L−1) kinetin concentrations in the medium; however, 0.5 mg·L−1 kinetin should be preferred in the case of production of multiple rooted microshoots during one stage. The addition of NaCl at relatively low concentrations (2.5 or 5.0 g·L−1) in a medium supplemented with 0.5 mg·L−1 BA doubled shoot multiplication but did not improve shoot elongation (100% explant response, 16 shoots per explant, 0.8 cm shoot length). For in vitro rooting, half-strength MS medium supplemented with 1.0 mg·L−1 IBA was most appropriate (97% rooting, 9.4 roots per microshoot, 1.2 cm root length). Regarding the effects of NaCl on in vitro rooting, microshoots were relatively tolerant to NaCl concentrations up to 10.0 g·L−1. The effects of NaCl depend on the micropropagation stage; they are synergistic during shoot multiplication and tolerant during rooting. However, explants responded satisfactorily in its absence, indicating that NaCl was not necessary as a medium component. Ex vitro acclimatization and establishment of plantlets was 100% successful in a mixture of peat:perlite 1:1 or 2:1 (v/v).

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Section: Discussionsupporting

confidence: 44%

In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte Limoniastrum monopetalum

Martini¹,

Papafotiou²

2020

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“…Microshoots rooted at 95% on half-strength Hf MS medium (Table 6, Figure 1J). Although, auxins are known to play an important role in root induction through their effect on first cell divisions that lead to root formation (Farooq et al, 2008), microshoots of a number of Mediterranean xerophytes, as A. barba-jovis, Ballota acetabulosa, C. nepeta, L. monopetalum (Papafotiou and Martini, 2016;Vlachou et al, 2016aVlachou et al, , 2017aVlachou et al, , 2017b, have been found to root on full-or half-strength MS medium without an auxin. Half-strength MS medium is quite often used successfully, as in the present study, since rather low nutrient concentrations positively affects rooting (Murashige, 1979;Saha et al, 2011;Vlachou et al, 2017aVlachou et al, , 2017bZhang et al, 2017).…”

Section: In Vitro Rooting and Ex Vitro Acclimatizationmentioning

confidence: 99%

“…The use of seedling-origin explants for in vitro cultures initiation usually results in high proliferation rate in Mediterranean species (Papafotiou et al, 2013;Papafotiou and Martini, 2016). Further, propagation by seed enhances genetic diversity that is desirable when native plants are used for natural landscape restoration, and contributes to the selection of genotypes with high pharmaceutical value (Sarasan et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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“…Micropropagation could facilitate the introduction of suitable clones to the pharmaceutical industry as well as breeding programs. The use of seedlings as stock plant material for micropropagation could lead to a high proliferation rate like in other Mediterranean native species (Papafotiou and Martini, 2016;Papafotiou et al, 2013). Further, the use of seedlings for either in vitro or ex vitro propagation could enhance the higher genetic diversity, which is desirable when native plants are reintroduced in the landscape, or contribute to the selection of particular genotypes of high medicinal value (Sarasan et al, 2011).…”

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confidence: 99%

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

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Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

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In Vitro Seed and Clonal Propagation of the Mediterranean Aromatic and Medicinal Plant Teucrium capitatum

Cited by 15 publications

References 52 publications

In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte Limoniastrum monopetalum

In Vitro Propagation and NaCl Tolerance of the Multipurpose Medicinal Halophyte Limoniastrum monopetalum

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

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