“…Acetate to propionate ratio was decreased by addition of either PEH or PEM compared to the control. This is consistent with a study (20) that showed that using P. kurroa aqueous extract increased propionate production and decreased acetate to propionate ratio. In contrast to our result, in another study (18) it was reported that acetate to propionate ratio was increased by addition of 50 and 100 g of gallic acid and tannic acid per kg DM compared to the control.…”
Section: Discussionsupporting
confidence: 82%
“…In agreement with the present study, it has been reported that addition of tannic acid to alfalfa hay had no influence on total VFAs (18). In contrast, in another study (20) it was reported that total VFAs were increased by addition of Plumbag zeylanica and Terminalia bellerica aqueous extracts. The increase in propionate concentration with the addition of either PEH15 or PEM30 could be Figure.…”
Section: Discussionsupporting
confidence: 70%
“…Other authors have observed similar decreases in the butyrate proportion using 50 and 100 g/kg tannic acid and quebracho tannin (18). In contrast, other researchers have detected increases in the butyrate proportion by Zingiber officinale aqueous and Moringa oleifera aqueous methanol extracts (2 mg/mL) (20). Acetate to propionate ratio was decreased by addition of either PEH or PEM compared to the control.…”
Section: Discussionmentioning
confidence: 70%
“…In contrast, other research (19) noted that addition of different extracts of Leucaena leucocephala and Salix babylonica extracts, (0.6, 1.2, 1.8 mL extract/g DM) containing a low TP concentration (<5% of DM) and saponin increased gas volume GP 24 , TDS, and MP versus the control. Another study (20) indicated that using extracts of Moringa oleifera aqueous methanol and Picrorhiza kurroa aqueous decreased apparent DM degradability and GP 24 but had no effect on MP. It is likely that the variation between extract effects could be due to the chemical nature of their active compounds, their activities, and their concentration (2).…”
“…Acetate to propionate ratio was decreased by addition of either PEH or PEM compared to the control. This is consistent with a study (20) that showed that using P. kurroa aqueous extract increased propionate production and decreased acetate to propionate ratio. In contrast to our result, in another study (18) it was reported that acetate to propionate ratio was increased by addition of 50 and 100 g of gallic acid and tannic acid per kg DM compared to the control.…”
Section: Discussionsupporting
confidence: 82%
“…In agreement with the present study, it has been reported that addition of tannic acid to alfalfa hay had no influence on total VFAs (18). In contrast, in another study (20) it was reported that total VFAs were increased by addition of Plumbag zeylanica and Terminalia bellerica aqueous extracts. The increase in propionate concentration with the addition of either PEH15 or PEM30 could be Figure.…”
Section: Discussionsupporting
confidence: 70%
“…Other authors have observed similar decreases in the butyrate proportion using 50 and 100 g/kg tannic acid and quebracho tannin (18). In contrast, other researchers have detected increases in the butyrate proportion by Zingiber officinale aqueous and Moringa oleifera aqueous methanol extracts (2 mg/mL) (20). Acetate to propionate ratio was decreased by addition of either PEH or PEM compared to the control.…”
Section: Discussionmentioning
confidence: 70%
“…In contrast, other research (19) noted that addition of different extracts of Leucaena leucocephala and Salix babylonica extracts, (0.6, 1.2, 1.8 mL extract/g DM) containing a low TP concentration (<5% of DM) and saponin increased gas volume GP 24 , TDS, and MP versus the control. Another study (20) indicated that using extracts of Moringa oleifera aqueous methanol and Picrorhiza kurroa aqueous decreased apparent DM degradability and GP 24 but had no effect on MP. It is likely that the variation between extract effects could be due to the chemical nature of their active compounds, their activities, and their concentration (2).…”
“…When foliage of shrubby species combines, high organic matter (OM) digestibility and low CH 4 production are potentially able to reduce the enteric CH 4 and increase ruminant productivity. The knowledge of the CH4 levels produced by the two native shrubby species should allow producers to adapt their production systems in order to reduce the emissions of this compound; for this reason, decreasing losses of CH4 have become a research priority (Alexander et al, 2008). The main sources of metabolisable protein are undegraded protein and microbial protein reaching the duodenum, being the microbial protein the most important part.…”
In order to evaluate the nutritional value of Parkinsonia texana and Acacia amentacea, two leguminosae species of the Tamaulipan scrubland, Northeastern Mexico, two experiments were carried out: the first tested the effects of season and browse species on chemical composition as nutritional variable to small ruminants; the second tested the effect of the addition of polyethylene glycol (PEG) on fermentation parameters. Foliage samples were collected from three sites. Data of chemical composition were analysed using analysis of variance for a bi-factorial arrangement, whereas the effect of PEG was analysed by a strip plot design. Results of chemical composition were affected by interacting factors season*species as individually they were significantly different (P<0.001). Addition of PEG affected (P<0.001) fermentation parameters. Significantly higher values of neutral detergent fibre (42%), condensed tannins (19%), purines (9 mol), partitioning factor (PF) (6.1) and gross energy losses (GEL=6.7%) were found in A. amentacea, while P. texana gave higher crude protein (18%), in vitro true organic matter digestibility (82%), metabolisable energy (ME) [2.1 Mcal/kg dry matter (DM)], A (183 mL), c (0.07/h) and L (0.86 h). Addition of PEG increased ME, and affected (P<0.001) fermentation parameters A and c, while purines and PF decreased. Results indicate that chemical composition and fermentation parameters vary according to seasons and species. PEG addition increases the fermentation parameters, which indicates that PEG counteracts the detrimental effects of secondary components of samples. Data suggest that using both species combined could supply necessary nutritional requirements to small ruminants in the Tamaulipan scrubland.
We developed an analytical method for screening vasoconstriction inhibitors from traditional Chinese medicines (TCMs) by combining vascular smooth muscle/cell membrane chromatography (VSM/CMC) with liquid chromatography-tandem mass spectrometry (LC-MS/MS). Primary cultured VSM cells from rat thoracic aortas were used for preparation of the stationary phase of the VSM/CMC column. Retention fractions from the VSM/CMC column were collected and then analyzed by LC-MS/MS under the optimized conditions offline. The suitability and reliability of the VSM/CMC-offline-LC-MS/MS method was assessed using nitrendipine and nifedipine as positive controls, and this method was then applied to screen vasodilator components from the extracts of Fructus Schisandrae Chinensis (FSC) and Fructus Schisandrae Sphenantherae (FSS). The major components from both species retained by VSM/CMC were identified as deoxyschizandrin (DSD) and schisantherin A (STA) by LC-MS/MS. Competition experiments indicated that DSD and nifedipine bound competitively to membrane receptors, while DSD and STA had partly overlapping binding sites on VSM-cell membranes. In vitro pharmacological trials confirmed that STA and DSD could dose-dependently relax the rat thoracic aortas pre-contracted by KCl. Our VSM/CMC-offline-LC-MS/MS method can be applied for screening vasoconstriction inhibitors from TCMs collected from FSC and FSS, and may be useful in the development of vasodilators from natural products.
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