In vitro Screening of Chinese Medicinal Toothpastes: Their Effects on Growth and Plaque Formation of Mutans Streptococci

Abstract

Many plant extracts or derivatives have been incorporated into commercial toothpastes to treat oral diseases related to caries or periodontal diseases in China. However, no information is available concerning their in vitro effects on oral bacteria. Thirty-one Chinese medicinal toothpastes were selected for this study Their ability to inhibit growth, in vitro plaque formation and glucosyltransferase (GTF) activity of Streptococcus sobrinus B13 and Streptococcus mutans 3209 were examined. Eighty-seven percent of the tested toothpastes inhibited the growth of the mutans streptococci, with zones ranging from 0.8 to 2.5 cm. At 10 mg/ml, 74% of the toothpastes inhibited in vitro plaque formation by S. mutans. Among these, 60% completely suppressed water-insoluble glucan synthesis from sucrose by GTF. Based on data obtained from our study, the incorporation of natural plant products or their derivatives into dentifrices seems a feasible means of promoting oral health and controlling dental diseases.

Antimicrobial Efficacy of Ten Commercially Available Herbal Dentifrices against Specific Oral Microflora – In Vitro Study

“…However, relatively few published articles in the literature have compared the effect of herbal based dentifrices on plaque associated bacteria [13][14][15][16][17]. Hence, the present study was carried out to determine and compare the antimicrobial efficacy of ten commercially available herbal dentifrices against specific strains of oral microflora using a standard diffusion method at full strength and 1:1 dilution at 24 h.…”

“…Probably, the active ingredients of the herbal dentifrices penetrate the biofilm and prevent plaque accumulation, thereby potentially preventing the colonization of the oral bacteria on the tooth surfaces [23]. However, very few studies [4,13,15] have evaluated the microbial efficacy of commercially available herbal dentifrices against oral microflora. Therefore, this study was aimed to determine the zones of inhibition formed by ten herbal dentifrices against specific oral microflora using the standard diffusion method at Fs and 1:1 dilution at 24 h.…”

Effects of <i>Apis mellifera</i> Propolis on the Activities of Streptococcal Glucosyltransferases in Solution and Adsorbed onto Saliva–Coated Hydroxyapatite

“…Many of them have pharmacological effects, such as antimicrobial, anti-inflammatory and cytostatic properties, among others [Wu-Yuan et al, 1990].…”

Estudo do efeito in vitro de extratos do rizoma de Dorstenia asaroides Hook. sobre fatores cariogênicos de Streptococcus mutans

“…Este fato tem levado pesquisadores de diversas áreas a buscar alternativas em produtos naturais empregados na medicina popular de diversas regiões do mundo (WU-YUAN et al, 1990;OOSHIMA et al, 2000;LEITÃO et al, 2004;LEITÃO et al, 2005). …”

“…Entre as propriedades farmacológicas citadas tem-se a antimicrobiana, antiinflamatória e citostáticas, entre outras (WU-YUAN et al, 1990;OOSHIMA et al, 2000;ITO et al, 2003).…”