In vitro screening for antiplasmodial activity of isoquinoline alkaloids from Brazilian plant species

Fischer, Dominique Corinne Hermine; Gualda, Nilton Carlos de Amorim; Bachiega, Danielle; Carvalho, Cyntia Salerno; Lupo, Fernanda Nogueira Gonçalves; Bonotto, Sonia Valéria; Alves, Mariana de Oliveira; Yogi, Álvaro; Santi, Sílvia Maria Di; Avila, Priscilla Elisangela; Kirchgatter, Karin; Moreno, Paulo Roberto Hrihorowitsch

doi:10.1016/j.actatropica.2004.08.009

Cited by 52 publications

(41 citation statements)

References 29 publications

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“…In our previous studies, in vitro antimalarial (Fischer et al, 2004), anti-leishmanial and trypanocidal (Tempone et al, 2005) activities were found for the crude ethanol extract and for the total alkaloids.…”

Section: Introductionmentioning

confidence: 99%

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

Siqueira

Oliani

Sartoratto³

et al. 2011

Rev. bras. farmacogn.

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Abstract:The essential oil of the leaves from Annona coriacea Mart., Annonaceae, was extracted by hydrodistillation in a Clevenger apparatus and analyzed by GC/MS and GC/FID. The oil yield was 0.05% m/m. Sixty compounds were identified, in a complex mixture of sesquiterpenes (76.7%), monoterpenes (20.0%) and other constituents (3.3%). Bicyclogermacrene was its major compound (39.8%) followed by other sesquiterpenes. Most of the monoterpenes were in low concentration (<1%). Only β-pinene and pseudolimonene presented the highest level of 1.6%. The volatile oil presented anti-leishmanial and trypanocidal activity against promastigotes of four species of Leishmania and trypomastigotes of Trypanosoma cruzi, showing to be more active against Leishmania (L.) chagasi (IC50 39.93 µg/mL) (95% CI 28.00-56.95 µg/mL).

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Section: Introductionmentioning

confidence: 99%

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

Siqueira

Oliani

Sartoratto³

et al. 2011

Rev. bras. farmacogn.

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“…Isoquinoline-containing natural products are widely used in biology and medicine because of their potent activities in a large number of biological assays (4,7,13,21). Saframycins represent a class of isoquinoline alkaloids exhibiting antibiotic and antitumor activities (1).…”

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confidence: 99%

Characterization of SafC, a Catechol 4- O -Methyltransferase Involved in Saframycin Biosynthesis

Nelson¹,

Lee

Sims³

et al. 2007

Appl Environ Microbiol

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Members of the saframycin/safracin/ecteinascidin family of peptide natural products are potent antitumor agents currently under clinical development. Saframycin MX1, from Myxococcus xanthus, is synthesized by a nonribosomal peptide synthetase, SafAB, and an O-methyltransferase, SafC, although other proteins are likely involved in the pathway. SafC was overexpressed in Escherichia coli, purified to homogeneity, and assayed for its ability to methylate a variety of substrates. SafC was able to catalyze the O-methylation of catechol derivatives but not phenols. Among the substrates tested, the best substrate for SafC was L-dihydroxyphenylalanine (L-dopa), which was methylated specifically in the 4-O position (k cat /K m ‫؍‬ 5.5 ؋ 10 3 M ؊1 s ؊1 ). SafC displayed less activity on other catechol derivatives, including catechol, dopamine, and caffeic acid. The more labile L-5-methyldopa was an extremely poor substrate for SafC (k cat /K m ‫؍‬ ϳ2.8 ؋ 10 ؊5 M ؊1 s ؊1 ). L-Dopa thioester derivatives were also much less reactive than L-dopa. These results indicate that SafC-catalyzed 4-O-methylation of L-dopa occurs prior to 5-C-methylation, suggesting that 4-O-methylation is likely the first committed step in the biosynthesis of saframycin MX1. SafC has biotechnological potential as a methyltransferase with unique regioselectivity.

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“…Alkaloids and flavonoids have already been isolated from the aerial parts of D. furfuracea and identified (Carollo et al 2006a). Furthermore, many biological activities of this plant species were reported, such as toxicity in mice embryo (Toledo et al 2006), trypanocidal, antiplasmodial, and antiprotozoal properties (Carollo et al 2006a, Fischer et al 2004, Mesquita et al 2007). …”

Section: Introductionmentioning

confidence: 99%

Assessment of Duguetia furfuracea genotoxic and cytotoxic activity in bacteria and mice

Silva¹,

Vieira²,

Santos³

et al. 2012

An. Acad. Bras. Ciênc.

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Duguetia furfuracea (St. Hil.) Benth & Hook f. (1862), popularly known as "sofre-do-rim-quem-quer" and "araticum-seco", is a shrub of the Annonaceae family that occurs in several regions of Brazil. In folk medicine the infusion of its leaves and twigs is used to treat rheumatism and renal colic, whereas the seed powder is mixed with water to treat pediculosis. Previous studies have described biological activities of this plant with cytotoxic, antitumoral, trypanocidal, leishmanicidal, antiplasmodial and antiprotozoal effects. In the present work, genotoxicity and cytotoxicity of Duguetia furfuracea lyophilized leaf extract were evaluated using the prophage λ induction test (SOS-Inductest) and mouse bone marrow micronucleus test. Our results showed that Duguetia furfuracea lyophilized leaf extract did not present an increase either in the induction of prophage λ (P>0.05) using the SOS-inductest or in the micronucleated polychromatic erythrocytes (P>0.05) using the micronucleus test, suggesting absence of genotoxicity in both tests. On the other hand, a significant decrease in the number of bacteria, (P<0.05), as well as a significant decrease in the polychromatic erythrocytes and normochromatic erythrocytes ratio, (P<0.05), were observed, showing the cytotoxic action of Duguetia furfuracea lyophilized leaf extract. Thus, Duguetia furfuracea did not present the genotoxic action, but showed a cytotoxic effect in both assays utilized in the present work.

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In vitro screening for antiplasmodial activity of isoquinoline alkaloids from Brazilian plant species

Cited by 52 publications

References 29 publications

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

Chemical constituents of the volatile oil from leaves of Annona coriacea and in vitro antiprotozoal activity

Characterization of SafC, a Catechol 4- O -Methyltransferase Involved in Saframycin Biosynthesis

Assessment of Duguetia furfuracea genotoxic and cytotoxic activity in bacteria and mice

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