In vitro screening for antioxidant and antimicrobial properties of three lebanese medicinal plants crude extracts

Alhage, Josiane; Elbitar, Hoda

doi:10.4103/pr.pr_171_18

Cited by 12 publications

(3 citation statements)

References 2 publications

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“…To the best of our knowledge, there is no scientific report on the effects of A. setosa on Salmonella in the literature. However, contrary to the present study's results, weak to moderate antioxidant potential and no significant antimicrobial for A. setosa have been reported (34,35). On the other hand, the chemical composition of the methanolic extract of Alcea setosa from Jordan showed 290 compounds, among which flavonoids (flavones) were diversified (34).…”

Section: The H Sabdariffa Calyx Extract Efficacy Againstcontrasting

confidence: 99%

Antimicrobial Effects of Medicinal Plant Species on Salmonella typhimurium Strains Isolated from Poultry Feces Samples

Dehghani,

Saeidi

2023

Jentashapir J Cell Mol Biol

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Background: It has been proven that plant extracts show great promise in fighting pathogenic microorganisms. This study aimed to evaluate the resistance of 20 strains of Salmonella typhimurium extracted from poultry feces against conventional antibiotics and the antibacterial activity of 10 medicinal plant extracts, including Hibiscus sabdariffa L., Capparis spinosa L., Azadirachta indica A. Juss., Eryngium planum L., Rumex acetosa L., Calotropis procera (Aiton) Dryand, Psidium guajava L., Malva sylvestris L., Urtica dioica L., and Alcea setosa Alef., against the extracted strains. Methods: The susceptibility of S. typhimurium strains against tested antibiotics was determined using disk diffusion, and the antibacterial activity of medicinal plant extracts was evaluated using well diffusion and broth microdilution assays. Results: The extracted S. typhimurium strains showed high resistance to cephalosporin (100%) and gentamicin (40%); however, all plant extracts examined in this study were influential in inhibiting the growth of the tested strains. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of tested plant extracts ranged from 6.25 to 25 mg/mL and 12.5 to 50 mg/mL, respectively. The most effective plant extracts in inhibiting bacterial growth in the agar well diffusion method were P. guajava, H. sabdariffa, and A. setosa; nevertheless, the most potent bactericidal activity was recorded for M. sylvestris and A. setosa in the broth microdilution method. The examined strains showed 80% and 85% sensitivity to the MBC of alcoholic extracts of M. sylvestris and A. setosa (50 mg/mL), respectively, which is worthy of further exploration by scientists. Conclusions: The results of this study represent the high potency of M. sylvestris and A. setosa extracts as appropriate medicinal and/or food supplements to replace ineffective antibiotics in bird breeding.

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Section: The H Sabdariffa Calyx Extract Efficacy Againstcontrasting

confidence: 99%

Antimicrobial Effects of Medicinal Plant Species on Salmonella typhimurium Strains Isolated from Poultry Feces Samples

Dehghani,

Saeidi

2023

Jentashapir J Cell Mol Biol

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“…The methanolic root extracts of A. luteus and A. microcarpus showed antimicrobial potential against methicillin-resistant S. aureus (MRSA), with MIC values of 650 to 1250 and 1250 to 2500 µg/mL, respectively [ 7 ]. Screening A. microcarpus tuber methanolic extract using an agar well diffusion assay revealed moderate activity against S. aureus, with an inhibition diameter zone of 14 mm [ 26 ]. Furthermore, the 80% hydromethanolic whole plant extract of A. tenuifolius was also found to be significantly active against S. aureus , E. coli , P. aeruginosa and K. pneumonia, with inhibition diameter zones of 16, 29, 18 and 18 mm, respectively, determined using the disc diffusion method [ 11 , 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…revealed moderate activity against S. aureus, with an inhibition diameter zone of 14 mm [26]. Furthermore, the 80% hydromethanolic whole plant extract of A. tenuifolius was also found to be significantly active against S. aureus, E. coli, P. aeruginosa and K. pneumonia, with inhibition diameter zones of 16, 29, 18 and 18 mm, respectively, determined using the disc diffusion method [11,13].…”

Section: Phytochemical Screening and Antimicrobial Activitymentioning

confidence: 99%

Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers

et al. 2023

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Root tubers of Asphodelus bento-rainhae subsp. bento-rainhae (AbR), a vulnerable endemic species, and Asphodelus macrocarpus subsp. macrocarpus (AmR) have traditionally been used in Portugal to treat inflammatory and infectious skin disorders. The present study aims to evaluate the in vitro antimicrobial activity of crude 70% and 96% hydroethanolic extracts of both medicinal plants, specifically against multidrug-resistant skin-related pathogens, to identify the involved marker secondary metabolites and also to assess the pre-clinical toxicity of these medicinal plant extracts. Bioguided fractionation of the 70% hydroethanolic extracts of both species using solvents of increasing polarity, namely diethyl ether (DEE: AbR-1, AmR-1), ethyl acetate (AbR-2, AmR-2) and aqueous (AbR-3, AmR-3) fractions, enabled the identification of the DEE fractions as the most active against all the tested Gram-positive microorganisms (MIC: 16 to 1000 µg/mL). Furthermore, phytochemical analyses using TLC and LC-UV/DAD-ESI/MS techniques revealed the presence of anthracene derivatives as the main constituents of DEE fractions, and five known compounds, namely 7′-(chrysophanol-4-yl)-chrysophanol-10’-C-beta-D-xylopyranosyl-anthrone (p), 10,7′-bichrysophanol (q), chrysophanol (r), 10-(chrysophanol-7′-yl)-10-hydroxychrysophanol-9-anthrone (s) and asphodelin (t), were identified as the main marker compounds. All these compounds showed high antimicrobial activity, particularly against Staphylococcus epidermidis (MIC: 3.2 to 100 µg/mL). Importantly, no cytotoxicity against HepG2 and HaCaT cells (up to 125 µg/mL) for crude extracts of both species and genotoxicity (up to 5000 µg/mL, with and without metabolic activation) for AbR 96% hydroethanolic extract was detected using the MTT and Ames tests, respectively. Overall, the obtained results contribute to the concrete validation of the use of these medicinal plants as potential sources of antimicrobial agents in the treatment of skin diseases.

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Health Potential of Zhourat Middle East Herbal Tea

Serhan,

Beyrouthy,

Menhem

et al. 2024

Reference Series in Phytochemistry

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In vitro screening for antioxidant and antimicrobial properties of three lebanese medicinal plants crude extracts

Cited by 12 publications

References 2 publications

Antimicrobial Effects of Medicinal Plant Species on Salmonella typhimurium Strains Isolated from Poultry Feces Samples

Antimicrobial Effects of Medicinal Plant Species on Salmonella typhimurium Strains Isolated from Poultry Feces Samples

Bioguided Identification of Active Antimicrobial Compounds from Asphodelus bento-rainhae and Asphodelus macrocarpus Root Tubers

Health Potential of Zhourat Middle East Herbal Tea

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