In vitro screening and in silico prediction of antifungal metabolites from rhizobacterium Achromobacter kerstersii JKP9

Karuppiah, Vijay; Devi, Thangarasu Suganya; Sree, Karthikeyan Kirupa; Elgorban, Abdallah M.; Kumar, Ponnuchamy; Govarthanan, Muthusamy; Thangavel, Kavitha

doi:10.1007/s00203-020-01982-0

Cited by 14 publications

(15 citation statements)

References 36 publications

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“…SUBG005 from phyla Actinobacteria and Agrobacterium larrymoorei from the Proteobacteria phyla were the subsequently most abundant bacteria. There have been reports of members of the genus Achromobacter employed as biocontrol agents against Fusarium oxysporum causing wilt in other plants [67,68]. The role of the microbes from this genus could be explored for their biocontrol potential against F. oxysporum.…”

Section: Discussionmentioning

confidence: 99%

Microbiome Analysis of the Rhizosphere from Wilt Infected Pomegranate Reveals Complex Adaptations in Fusarium—A Preliminary Study

Das

Ravinath

Usha³

et al. 2021

Agriculture

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Wilt disease affecting pomegranate crops results in rapid soil-nutrient depletion, reduced or complete loss in yield, and crop destruction. There are limited studies on the phytopathogen Fusarium oxysporum prevalence and associated genomic information with respect to Fusarium wilt in pomegranate. In this study, soil samples from the rhizosphere of different pomegranate plants showing early stage symptoms of wilt infection to an advanced stage were collected from an orchard situated in Karnataka, India. A whole metagenome sequencing approach was employed to gain insights into the adaptations of the causative pathogen F. oxysporum. Physicochemical results showed a drop in the pH levels, N, Fe, and Mn, and increase in electrical conductivity, B, Zn, Cl, Cu was observed in the early and intermediate stage samples. Comparative abundance analysis of the experimental samples ESI and ISI revealed an abundance of Proteobacteria phyla Achromobacter sp. 2789STDY5608625, Achromobacter sp. K91, and Achromobacter aegrifaciens and Eukaryota namely Aspergillus arachidicola, Aspergillus candidus, and Aspergillus campestris. Functional pathway predictions implied carbohydrate binding to be significant (p < 0.05) among the three experimental samples. Microbiological examination and whole microbiome analysis confirmed the prevalence of F. oxysporum in the soil samples. Variant analysis of F. oxysporum revealed multiple mutations in the 3IPD gene with high impact effects. 3-Isopropylmalate dehydratase and carbohydrate-active enzymes could be good targets for the development of antifungals that could aid in biocontrol of F. oxysporum. The present study demonstrates the capabilities of the whole metagenome sequencing approach for rapid identification of potential key players of wilt disease pathogenesis wherein the symptomatology is complex.

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Section: Discussionmentioning

confidence: 99%

Microbiome Analysis of the Rhizosphere from Wilt Infected Pomegranate Reveals Complex Adaptations in Fusarium—A Preliminary Study

Das

Ravinath

Usha³

et al. 2021

Agriculture

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“…Statistical validation was established by repeated inoculation thrice and measurement of colony to zone diameter aspect ratio to identify the potent (Cui et al, 2013). Plant‐growth‐promoting activities, such as phosphate solubilization, production of IAA, ammonia, HCN, lipases, proteases, amylases, cellulases and chitinases, were screened based on methods adopted by Vijay et al (2020). Potential rhizobacteria were identified based on 16S gene sequence homology and the assembled sequences were submitted to GenBank.…”

Section: Methodsmentioning

confidence: 99%

Development of siderophore-based rhizobacterial consortium for the mitigation of biotic and abiotic environmental stresses in tomatoes: Anin vitroandin plantaapproach

Karuppiah

Suganthy

Muralitharan

et al. 2022

Journal of Applied Microbiology

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Aim Tomato‐associated plant‐growth‐promoting rhizosphere bacteria were screened for effective antagonistic activity against the fungal vascular wilt pathogens; tolerance to heavy metals; and enhancing the bioavailability of iron for tomato plants through in vitro and in vivo approaches. Methods and Results Among the 121 rhizobacteria screened for siderophores, 25 isolates were observed to be siderophore producers and out of these, seven isolates chelate copper and iron thus exhibiting in vitro antagonism against the virulent strains of Fusarium oxysporum f. sp. lycopersici MTCC10270 (Fol), Fusarium equiseti MFol and Sarocladium sp. SWL isolated from infected tomatoes. Pseudomonas stutzeri KRP8 was identified to be the most potent strain among the siderophore producers and its siderophores were chemically characterized by mass spectra as metal bound and metal‐free forms. Upon bio‐inoculation of fortified bacterial consortium (siderozote) into the rhizosphere of vermiculite pot cultured tomatoes supplied with varying concentrations of iron and copper ions, we observed in planta growth improvements, antagonism, enhancement of bioavailability of iron and heavy metal tolerance using Inductively Coupled Plasma‐Optical Emission Spectrometry. Conclusion and Significance of the Study Our rhizobacterial consortium provides an opportunity for soil reclamation through an ecofriendly method for a heavy metal‐free agricultural landscape.

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“…[27,28] The large size and high boiling point of pyrrolopyrazines, compared to other compounds, make them less susceptible to volatilizing when subject to the temperatures used in the headspace (in Solid Phase Micro Extraction (SPME), for example), thus also making this class more prominent for liquid-liquid extraction (LLE). [26,[29][30][31][32][33][34] Approximately 34 articles describing pyrrolo-pyrazines from bacterial isolates were found in the scientific database (Table 1). However, not many described the purification and identification methods for nitrogenous compounds or their biological activity.…”

Section: Pyrrolo-pyrazinesmentioning

confidence: 99%

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

Valença

Barbosa

Souto

et al. 2021

Chemistry & Biodiversity

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Bacteria can produce nitrogenous compounds via both primary and secondary metabolic processes. Many bacterial volatile nitrogenous compounds produced during the secondary metabolism have been identified and reported for their antioxidant, antibacterial, antifungal, algicidal and antitumor activities. The production of these nitrogenous compounds depends on several factors, including the composition of culture media, growth conditions, and even the organic solvent used for their extraction, thus requiring their identification in specific conditions. In this review, we describe the volatile nitrogenous compounds produced by bacteria especially focusing on their antimicrobial activity. We concentrate on azo-compounds mainly pyrazines and pyrrolopyridines reported for their activity against several microorganisms. Whenever significant, extraction and identification methods of these compounds are also mentioned and discussed. To the best of our knowledge, this is first review describing volatile nitrogenous compounds from bacteria focusing on their biological activity.

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In vitro screening and in silico prediction of antifungal metabolites from rhizobacterium Achromobacter kerstersii JKP9

Cited by 14 publications

References 36 publications

Microbiome Analysis of the Rhizosphere from Wilt Infected Pomegranate Reveals Complex Adaptations in Fusarium—A Preliminary Study

Microbiome Analysis of the Rhizosphere from Wilt Infected Pomegranate Reveals Complex Adaptations in Fusarium—A Preliminary Study

Development of siderophore-based rhizobacterial consortium for the mitigation of biotic and abiotic environmental stresses in tomatoes: Anin vitroandin plantaapproach

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

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