In vitro screen of Lactobacillus plantarum as probiotic bacteria and their fermented characteristics in soymilk

Bao, Yan; Zhang, Yong; Li, Haiping; Liu, Yong; Wang, Shuiquan; Dong, Xin; Su, Fang; Yao, Guoqiang; Sun, Tingting; Zhang, Heping

doi:10.1007/s13213-011-0377-4

Cited by 53 publications

(31 citation statements)

References 31 publications

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“…L. plantarum P‐8, also named IMAU10120, was screened out from 102 L. plantarum strains isolated in our laboratory from traditional fermented foods 15. Before daily administration, L. plantarum P‐8 was reconstituted in sterile water, for 15 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Effect of Lactobacillus plantarum P‐8 on lipid metabolism in hyperlipidemic rat model

Bao

Wang

Zhang

et al. 2012

Euro J Lipid Sci & Tech

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Probiotics have been reported to play an important role in the prevention of metabolic disorders. We recently identified a novel probiotic strain Lactobacillus plantarum (L. plantarum) P-8. The objective of this study was to determine the effects of L. plantarum P-8 on lipid metabolism of rats fed with high fat diet. All experimental rats were divided into three groups: control group, model group, and L. plantarum P-8 group. Changes in serum lipid levels, hepatic lipid deposition, serum oxidative stress-related parameters, activities of liver function marker enzymes, organ indices, gut microflora, and fecal lipids were assessed. Compared with model group, L. plantarum P-8 exhibited hypolipidemic effects by lowering serum total cholesterol (TC), triglyceride (TG), and low-density lipoprotein cholesterol levels, accompanied with elevation of high-density lipoprotein cholesterol level. L. plantarum P-8 also exerted beneficial effects against high-fat diet-induced oxidative stress, curtailed the accumulation of liver lipids and protected healthy liver function. Moreover, L. plantarum P-8 was able to regulate intestinal bacteria and enhance the fecal excretion of TC, TG, and bile acid. These findings indicate that L. plantarum P-8 may represent a potential therapeutic agent for controlling hyperlipidemia.

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Section: Methodsmentioning

confidence: 99%

Effect of Lactobacillus plantarum P‐8 on lipid metabolism in hyperlipidemic rat model

Bao

Wang

Zhang

et al. 2012

Euro J Lipid Sci & Tech

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“…Clade I contained seven isolates from four STs (ST38, ST54, ST64 and ST67) of which six isolates came from pickles. Clade II contained isolates with the greatest diversity of ecological sources, although isolates in ST3 all originated from fermented dairy foods including reference isolate CGMCC6312 [ 25 ]. Clade III was composed of five STs, each from a different region, but all containing isolates from fermented dairy foods.…”

Section: Resultsmentioning

confidence: 99%

Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources

Liu

Zhang

et al. 2015

BMC Microbiol

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BackgroundLactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a novel MLST scheme employing eight housekeeping genes. These isolates had originated from different sources and geographic regions: 179 isolates were from our own culture collection and originated from China and Mongolia and seven isolates were type or reference isolates from other collections.ResultsThe results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons. There were 158 polymorphic sites detected in total, and the nucleotide diversity per site varied from 0.00401 in clpX to 0.03220 in groEL. The minimum spanning tree analyses suggested that the evolution of L. plantarum isolates have little relationship with ecological sources have similar nucleotide diversity. Phylogenetic trees and structure indicated that there were six lineages in the L. plantarum isolates used in our study. Split-decomposition and ClonalFrame analysis indicated that recombination had occurred throughout the population of L. plantarum, but it occurred at a low frequency in these eight loci.ConclusionWe deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure. The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made. This will further advance our understanding of the microbial ecology of this industrially important LAB.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-015-0584-4) contains supplementary material, which is available to authorized users.

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“…The L. plantarum P8 strain (IMAU10120) was used as the control in this experiment. It is a well-characterized strain that has high gastrointestinal transit survival and confers numerous desirable effects to the host ( Bao et al, 2012 ; Wang et al, 2014 ). Among the six selected OPPs-degrading strains, P9 matched most to the gastrointestinal tolerance property of L. plantarum P8, with a high survival in simulated gastric juice of pH 2.5 for 3 h (62.74 ± 0.82%), intestinal juice for 4 h (93.16 ± 7.85%) and 8 h (89.08 ± 4.94%).…”

Section: Discussionmentioning

confidence: 99%

“…The gastric transit tolerance was estimated based on the bacterial survival rate, which was calculated from the total viable counts prior to and 3 h after incubation in simulated gastric juice, as well as after 4 and 8 h of incubation in simulated intestinal juice. The probiotic L. plantarum P8 ( Bao et al, 2012 ; Wang et al, 2014 ) was used as a reference strain in this experiment. The experiment was performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

Screening for Lactobacillus plantarum Strains That Possess Organophosphorus Pesticide-Degrading Activity and Metabolomic Analysis of Phorate Degradation

et al. 2018

Front. Microbiol.

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This work performed a large scale assessment for organophosphorus pesticides (OPPs) degradation activity of 121 Lactobacillus (L.) plantarum strains. Six L. plantarum strains (P9, IMAU80110, IMAU40100, IMAU10585, IMAU10209, and IMAU80070) were found to possess high capacity of degrading three commonly used OPPs, namely dimethoate, phorate, and omethoate; and they were selected for more detailed characterization. Moreover, the three OPPs were mainly detected in the culture supernatants but not in the cell extracts, further confirming that the OPPs were degraded rather than absorbed by the cells. Among the six selected strains, P9 was most tolerant to gastrointestinal juices and bile. We thus used ultra-high performance liquid chromatography electron spray ionization coupled with time-of-flight mass spectrometry (UPLC/ESI-Q-TOF/MS) to generate the metabolomic profiles of the strain P9 growing in MRS medium with and without containing phorate. By using orthogonal partial least squares discriminant analysis, we identified some potential phorate-derived degradative products. This work has identified novel lactic acid bacteria resources for application in pesticide degradation. Our results also shed light on the phorate degradation mechanism by L. plantarum P9.

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In vitro screen of Lactobacillus plantarum as probiotic bacteria and their fermented characteristics in soymilk

Cited by 53 publications

References 31 publications

Effect of Lactobacillus plantarum P‐8 on lipid metabolism in hyperlipidemic rat model

Effect of Lactobacillus plantarum P‐8 on lipid metabolism in hyperlipidemic rat model

Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources

Screening for Lactobacillus plantarum Strains That Possess Organophosphorus Pesticide-Degrading Activity and Metabolomic Analysis of Phorate Degradation

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