In Vitro Scolicidal Effects of &lt;i&gt;Salvadora persica&lt;/i&gt; Root Extract against Protoscolices of &lt;i&gt;Echinococcus granulosus&lt;/i&gt;

Abdel‐Baki, Abdel‐Azeem S.; Al-Malki, Esam S.; Mansour, Lamjed; Al-Quarishy, Saleh

doi:10.3347/kjp.2016.54.1.61

Cited by 36 publications

(14 citation statements)

References 25 publications

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“…Livestock hosts such as sheep, camels and goats, are the essential reservoirs of hydatidosis disease for humans (Daryani et al, 2007). In this context, and since hydatidosis is of public health significance, as well as of economic importance in the Kingdom of Saudi Arabia (Hussein et al, 2012, Abdel-Baki et al, 2016), the objective of the present work was to study the prevalence of hydatidosis infection in slaughtered Sawakny sheep in Riyadh city slaughter houses.…”

Section: Discussionmentioning

confidence: 99%

Assessment of prevalence of hydatidosis in slaughtered Sawakny sheep in Riyadh city, Saudi Arabia

Al-Malki

Al-Quarishy

Abdel‐Baki

2017

Saudi Journal of Biological Sciences

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Hydatidosis, or echincoccosis, is a serious medical and veterinary problem in many countries, particularly those with rural communities where there is a greater contact between dogs and domestic animals. Domestic livestock act as intermediate hosts which are the main reservoir for the disease in humans. It is therefore very important to estimate the prevalence of hydatid cysts in slaughtered animals since it can be transmitted to humans through dogs, which act as the final host for the disease. From this point of view, the present study was suggested to determine the prevalence of hydatidosis in Sawakny sheep slaughtered in Riyadh city, Saudi Arabia. During the course of the study 12,569 Sawakny sheep were inspected for hydatidosis infection. An overall prevalence of 1.06% was detected among the examined sheep, with the highest prevalence occurring in winter (1.38%) and lowest prevalence in summer (0.67%). Sheep aged 6-12 months had a higher rate of infection than older animals, and males were the predominant carriers of infection (97.7%) compared to females (2.3%). The liver was the most infected organ (79.1%), followed by the lungs (14.6%), while concurrent infections of both the liver and the lungs occurred in 6% of cases. The fertility and viability rates of hydatid cysts in the liver (70.1% and 85.1% respectively) were higher than that in any other organs. In conclusion, it is evident that fertile cysts in slaughtered sheep could have an important role in the continuation of hydatid cyst transmission to humans through dogs. Considerable effort should be devoted to controlling the transmission of cysts from abattoirs by the secure disposal of infected offal. In addition, plans are required for further epidemiological studies and control programs.

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Section: Discussionmentioning

confidence: 99%

Assessment of prevalence of hydatidosis in slaughtered Sawakny sheep in Riyadh city, Saudi Arabia

Al-Malki

Al-Quarishy

Abdel‐Baki

2017

Saudi Journal of Biological Sciences

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“…However McManus et al (2003) have argued that there is no ideal agent which is both effective and safe. It is extremely important, therefore, to identify an effective alternative protoscolicidal agent, especially to overcome the severe side-effects of the synthetic pharmaceuticals (Moazeni and Nazer (2011); Abdel-Baki et al, 2016). Since herbal extracts have been recognised as having the potential to be effective and safe alternative agents (Elissondo et al, 2008), the present study has sought to investigate the protoscolicidal effect of ethanolic extracts of turmeric and ginger for use against protoscolices in hydatid cysts.…”

Section: Discussionmentioning

confidence: 99%

In vitro effectiveness of Curcuma longa and Zingiber officinale extracts on Echinococcus protoscoleces

Al-Malki

Al-Shaebi

Al-Quarishy

et al. 2017

Saudi Journal of Biological Sciences

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Hydatid disease is an important economic and human public health problem with a wide geographical distribution. Surgical excision remains the primary treatment and the only hope for complete cure of hydatosis. The most important complications arising from surgical excision, however, is recurrence, which is due to dissemination of protoscolices during the surgery. Pre-surgical inactivation of the contents of the hydatid cyst by injection of scolicidal agent into the cyst has been used as adjunct to surgery in order to overcome the risk of recurrence. In the present study, ethanolic extracts of turmeric () and ginger () were tested as scolicidal agent for protoscoleces. Protoscoleces were collected aseptically from sheep livers containing hydatid cysts. Three concentrations (10, 30 and 50 mg/ml) of each extract were investigated and viability of the protoscoleces was tested by 0.1% eosin staining. Ginger extract showed the strongest scolicidal effect (100%) after 20 min at a concentration of 30 mg/ml and 10 min at 50 mg/ml The maximum scolicidal effect of turmeric was 93.2% after 30 min at a concentration of 50 mg/ml. It is concluded that turmeric and ginger extracts have high scolicidal activity and could be used as effective scolicidal agents against protoscoleces.

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“…After treating for 4 days, PSCs were collected to evaluate the mortality via the eosin dye exclusion test, followed by monitoring the ultramicroscopic change by transmission electron microscopy (TEM, JEM-100CXII, Alignment, Japan) as previously described. 18 , 19 …”

Section: Methodsmentioning

confidence: 99%

<p>In vitro and in vivo Effects of Artesunate on <em>Echinococcus granulosus</em> Protoscoleces and Metacestodes</p>

Wen¹,

Lv²,

Zhao³

et al. 2020

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Background In this study, we aim to investigate the efficiency of artesunate (AS) on Echinococcus granulosus protoscoleces and metacestodes. Methods For the in vitro assay, the eosin dye exclusion test and transmission electron microscope (TEM) were utilized to evaluate the effects of AS against protoscoleces (PSCs) from Echinococcus granulosus . In addition, mortality, ultrastructure change, reactive oxygen species (ROS) content and DNA damage were measured in order to explore the anti-echinococcosis mechanism of AS. For the in vivo assay, CE-infected mice were divided into model group, albendazole (ABZ) group (200 mg/kg), low AS (AS-L) group (50 mg/kg), moderate AS (AS-M) group (100 mg/kg), and high AS (AS-H) group (200 mg/kg). Upon 6 weeks oral administration, wet weight of cysts and the ultrastructural changes of cystic wall were utilized to evaluate the effects of AS on metacestodes. In addition, the liver biochemical parameters, tumor necrosis factor-α (TNF-α), glutathione/glutathione oxidized (GSH/GSSG) ratio in serum, and H 2 O 2 , total superoxide dismutase (T-SOD) in cyst fluid were detected. Results Both in vivo and in vitro experiments showed that AS showed anti-parasitic effects on CE. The AS could elevate the ROS level in the PSCs, which then resulted in obvious DNA damages. AS could significantly improve the liver biochemical parameters in infected mice compared with the model group ( P < 0.05). Compared with the model group, AS-M and AS-H decrease the TNF-α content ( P < 0.05); AS-H group significantly decrease in the serum GSH/GSSG ratio ( P < 0.05). The content of H 2 O 2 in hydatid fluid treated by AS showed significant decrease compared with the model group ( P < 0.01), while the T-SOD level showed significant elevation compared with model group ( P < 0.01). Conclusion In this study, we confirmed that the effects of AS on Echinococcus granulosus protoscoleces and metacestodes may be related to the DNA damages induced by oxidative stress, which provided solid information for the research and development of drugs for cystic echinococcosis.

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In Vitro Scolicidal Effects of <i>Salvadora persica</i> Root Extract against Protoscolices of <i>Echinococcus granulosus</i>

Cited by 36 publications

References 25 publications

Assessment of prevalence of hydatidosis in slaughtered Sawakny sheep in Riyadh city, Saudi Arabia

Assessment of prevalence of hydatidosis in slaughtered Sawakny sheep in Riyadh city, Saudi Arabia

In vitro effectiveness of Curcuma longa and Zingiber officinale extracts on Echinococcus protoscoleces

<p>In vitro and in vivo Effects of Artesunate on <em>Echinococcus granulosus</em> Protoscoleces and Metacestodes</p>

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