In vitro rumen fermentation of soluble and non-soluble polymeric carbohydrates in relation to ruminal acidosis

Darwin, Darwin; Barnes, Anne; Cord‐Ruwisch, Ralf

doi:10.1007/s13213-017-1307-x

Cited by 14 publications

(18 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is established that lactic acid bacteria are effective starch degraders with the essential enzymes amylase and maltase [33][34][35], while many yeasts such as Saccharomyces are not effective starch degraders [36]. Interestingly the trend is reversed with starch as the feed.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly the trend is reversed with starch as the feed. It is established that lactic acid bacteria are effective starch degraders with the essential enzymes amylase and maltase [33][34][35], while many yeasts such as Saccharomyces are not effective starch degraders [36]. Some previous studies also found that many types of yeast are not able to degrade starch due to the fact that they are not able to produce extracellular amylase [37].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Ethanol and lactic acid production from sugar and starch wastes by anaerobic acidification

Darwin

Cord‐Ruwisch

Charles

2018

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Anaerobic conversion of carbohydrates can generate various end‐products. Besides physical parameters such as pH and temperature, the types of carbohydrate being fermented influences the fermentation pattern. Under uncontrolled pH, microbial mixed cultures from activated sludge and anaerobic digester sludge anaerobically produced ethanol from glucose while producing lactic acid from starch conversion. This trend was not only observed in batch trials. Also, continuous chemostat operation of anaerobic digester sludge resulted in the reproducible predominance of ethanol fermentation from glucose solution and lactic acid production from starch. Different feeding regimes and substrate availability (shock load versus continuous feeding) in glucose fermentation under non‐controlled pH did not affect the ethanol production as the major end product. Shifts in feed composition from glucose to starch and vice versa result in an immediate change of fermentation end products formation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Ethanol and lactic acid production from sugar and starch wastes by anaerobic acidification

Darwin

Cord‐Ruwisch

Charles

2018

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

show abstract

“…of productivity and yields of fermentation end-products [4]. This condition usually occurs in rumen fermenting carbohydrates [5,6]. Therefore, a practical and reliable method for monitoring glucose concentration in the fermentation broths is highly required.…”

Section: Introductionmentioning

confidence: 99%

Determination of Glucose Concentration in Anaerobic Acidification Cultures by Portable Glucose Monitoring System

Darwin

2019

Asian J. Chem.

View full text Add to dashboard Cite

In mixed microbial fermentation, sugar concentration should be monitored regularly in order to evaluate the effectiveness of fermentation process. Anaerobic acidification fermentation is the process involving microbes to convert the soluble carbohydrate (e.g. glucose) derived from the hydrolysis of insoluble carbohydrates (e.g. starch). The determination of glucose during the fermentation is essential in order to evaluate the mass balance of electron transfer from the oxidation of glucose to the fermentation end-products (e.g. organic acids and alcohols). The fast and practical measurements of glucose concentration in the fermentation broth are highly required to evaluate and ensure the stability of fermentation process. The results showed that once glucose as a soluble sugar is available in the fermentation broth, it was accurately measured by GlucoDr blood glucose biosensor. The standard curve of glucose solution showed the linear relationship between glucometer reading and glucose concentration in which the coefficient determination obtained was at about 0.99. This indicated that glucose analysis with using GlucoDr blood glucose biosensor was accurate and reproducible.

show abstract

“…However, very few studies have revealed that lactic acid accumulation during the acidogenesis phase is the primary reason for an upset digester. Recent studies have revealed that anaerobic acidogenesis of starch wastes could potentially produce lactic acid as the primary end-product (Darwin et al, 2018a;Darwin et al, 2018b), and thereby it could interfere with the process of anaerobic digestion. As shown previously, lactic acid build-up caused the pH to drop significantly compared to VFA build-up (Darwin et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

“…Recent studies have revealed that anaerobic acidogenesis of starch wastes could potentially produce lactic acid as the primary end-product (Darwin et al, 2018a;Darwin et al, 2018b), and thereby it could interfere with the process of anaerobic digestion. As shown previously, lactic acid build-up caused the pH to drop significantly compared to VFA build-up (Darwin et al, 2018b). This occurs because lactic acid has a lower pKa (3.85) compared to VFA (pKa 4.76-4.87) (O'Hanlon et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of lactic acid in anaerobic biological treatment process using a portable sensitive lactate biosensor

Darwin¹

2019

bta

View full text Add to dashboard Cite

The rapid and practical determination of lactic acid concentration during anaerobic digestion and in acidification samples is extremely important to ensure the digester is running properly and to avoid build-up of lactic acid. Thus, developing a simple and fast method for analyzing lactic acid in anaerobic digestion samples is important. The results of the present study revealed that lactic acid is an intermediate product of anaerobic digestion, which could be quickly measured using a portable lactate biosensor. We obtained linear standard curves from the relationship between various lactic acid concentrations of the standard solution and the lactate biosensor reading, which were tested at different temperatures (30, 45 and 55EC). A high value of coefficient determination,-0.98-0.99, was obtained from all standard curves. This suggests that the evaluated system was accurate, reliable, and reproducible.

show abstract

In vitro rumen fermentation of soluble and non-soluble polymeric carbohydrates in relation to ruminal acidosis

Cited by 14 publications

References 31 publications

Ethanol and lactic acid production from sugar and starch wastes by anaerobic acidification

Ethanol and lactic acid production from sugar and starch wastes by anaerobic acidification

Determination of Glucose Concentration in Anaerobic Acidification Cultures by Portable Glucose Monitoring System

Rapid determination of lactic acid in anaerobic biological treatment process using a portable sensitive lactate biosensor

Contact Info

Product

Resources

About