In vitro rooting of cloned shoots in <i>Pinus pinaster</i>

Rancillac, M.; Faye, M.; David, Alain

doi:10.1111/j.1399-3054.1982.tb04905.x

Cited by 25 publications

(18 citation statements)

References 10 publications

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“…(INRA, Laboratoire d'Ammelioration des Arbres Forestiers, Bordeaux, France) were used. Pine needles were produced by in vitro culture of cloned shoots ( 17).…”

Section: Biological Materialsmentioning

confidence: 99%

Artefactual Origins of Cyclic AMP in Higher Plant Tissues

Spiteri¹,

Viratelle²,

Raymond³

et al. 1989

Plant Physiol.

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A highly sensitive radioimmunoassay has been used to determine the levels of adenosine 3',5'-cyclic monophosphate (cAMP) in five higher plants (Lactuca sativa, Helianthus annuus, Oryza sativa, Pinus pinaster, Nicotiana tabacum). Particular attention was paid to the three main sources of errors in the charactenzation of cAMP in plants: presence of interfering substances in plant tissues; possible artefactual formation of cAMP from endogenous ATP during extraction, purification, and assay; and microbial origin of cAMP. In all the tested tissues, the cAMP level was below the detection limit of 0.5 picomole per gram fresh weight, a value much lower than those reported for similar materials of the same species in many previous studies. This result is not in favor of cAMP-dependent regulations in higher plants.et al. (7,8) to check the presence of cAMP in different plants chosen among gymnosperms, monocotyledons, and dicotyledons. Great care was taken to ensure the elimination of interfering compounds and the absence of de novo synthesis of cAMP during the assay, together with maximal recovery of endogenous cAMP. In all tissues, the cAMP level was* below the detection limit ofthe assay: the discrepancy between these results and published ones on similar materials is discussed in the light of potential sources of errors. MATERIALS AND METHODS Biological MaterialCyclic AMP is present in animal tissues and in many microorganisms such as bacteria, fungi, and green algae. Its occurrence in higher plants and its role in the regulation of their metabolism are still a matter of debate. The main evidence supporting the existence of the cAMP' system in plant tissues is based on cAMP estimations, but a large diversity exists among reported determinations of cAMP in higher plants: for example, in the case of tobacco callus, published values range from 900 (15) to less than 0.5 pmol. g-' (2). On the other hand, there is only scant evidence for enzymes involved in cAMP metabolism (9). Most data in this field can be found in reviews (9) Lettuce (Lactuca sativa L., cv Val d'Orge) was obtained from Societe Clause (France). Lettuce seeds were sterilized by soaking for 20 min in commercial sodium hypochlorite (150 g ofchlorine per L). After extensive washing with sterile water, seeds were either immediately frozen in liquid nitrogen or germinated for 2 h at 20C.Sunflower (Helianthus annuus L., cv Rodeo) was obtained from Cetiom (France). Different materials were used: immature seeds were removed from plant heads 7 and 14 d after self-pollination in a growth cabinet and nondesiccated mature seeds after 28 d; these seeds, as well as mature dry seeds, were soaked for 15 min in one-fourth diluted commercial sodium hypochlorite, dehusked, and further sterilized in one-tenth hypochlorite dilution; young seedlings were obtained 3 and 8 d after the beginning of germination under sterile conditions. Rice (Oryza sativa L., var Cigalon) was obtained from Station d'Amelioration des Plantes (INRA Montpellier, France). Dehusked dry seeds wer...

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“…(INRA, Laboratoire d'Ammelioration des Arbres Forestiers, Bordeaux, France) were used. Pine needles were produced by in vitro culture of cloned shoots ( 17).…”

Section: Biological Materialsmentioning

confidence: 99%

Artefactual Origins of Cyclic AMP in Higher Plant Tissues

Spiteri¹,

Viratelle²,

Raymond³

et al. 1989

Plant Physiol.

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“…It is generally considered that the rooting and acclimatization stages of microcuttings are the most critical steps in conifer micropropagation (Franclet et al, 1980;Rancillac et al, 1982;Mohammed and Vidaver, 1988 (Scaltsoyiannes, 1988). A large variation among clones was also observed by Aitken-Christie and Thorpe (1984) who worked on rooting of P radiata microcuttings and also by Kleinschmidt and Schmidt (1977) and Zobel and Talbert (1987) …”

Section: Rooting Of Shootsmentioning

confidence: 99%

“…Although progress in micropropagation of conifers through organogenesis from organ explants has been achieved, rooting of the micropropagated shoots (microcuttings) and the acclimatization of plantlets are still a problem (Jelaska, 1987;Mohammed and Vidaver, 1988;Stiff et al, 1989). According to many workers, further research on the influence of factors such as donor age, genotype, type of explant, microcutting quality, auxin treatment, root system and environmental conditions, on rooting and acclimatization is required (Franclet et al, 1980;Rancillac et al, 1982;Mohammed and Vidaver, 1988). …”

Section: Introductionmentioning

confidence: 99%

Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

et al. 1994

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Summary — Fascicle shoots proved to be an ideal plant material for micropropagation of the pine hybrid P brutia (Ten) x P halepensis (Mill). This could be possibly attributed to their morphological and physiological state. Induced fascicle shoots of 4-yr-old seedlings were used as explants. Their induction was achieved by spraying once with the new herbicide Arsenal (1 000 mg I -1 ). First, explants were elongated in vitro on LP medium and then transferred to the multiplication stage. Multiplication was accomplished by decapitating, quick-dipping in 0.22 mM BA and inoculating the explants on BIMI medium. On the induced first-generation shoots the same procedure was applied, in order to obtain second-generation shoots and then these were proceeded to the rooting and acclimatization stages. In particular, when microcuttings were pretreated with 2.46 μM IBA + 2.7 μM NAA + 0.65% agar w/v + 1.5% sucrose w/v for 7 d and then transferred to greenhouse conditions, a good root system was developed within an 8-12-week period. A large variation in rootability was noted between clones. The above method may be proved to be efficient for clones that exhibit high rooting ability.

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“…Other substrates, e.g. soilless mixtures of peat, perlite, vermiculite or sand, have posed problems with moisture control [121] and maintenance of good shoot health [114]. However, the quality of roots produced in agar is not always acceptable.…”

Section: In Vivo and In Vitro Rootingmentioning

confidence: 99%

“…Agar probably impedes gas exchange and inhibits the development of the vascular system in roots as well as the production of root hairs [132]. More permeable substrates such as peat:perlite or peat: vermiculite have been recommended [30,121] particularly for subsequent root growth. For rooting, nutrients are usually reduced to half the strength of that used for shoot production [20,34,36,84,104,105,112].…”

Section: In Vivo and In Vitro Rootingmentioning

confidence: 99%

Root production and plantlet development in tissue-cultured conifers

Mohammed¹,

Vidaver

1988

Plant Cell Tiss Organ Cult

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Rooting and acclimatization procedures for micropropagated conifers are reviewed, with emphasis on their effects on root quality and plantlet performance in the nursery and field. Major influences on root production include auxin concentration and mode of application, shoot quality, donor age, clone and temperature. The development of a fibrous, well-branched root system has been a problem that may be solved by using rooting substrates that are better-aerated than agar. Further development of the root system may be enhanced by early air-pruning and ectomycorrhizal associations. During acclimatization, high humidity is required for conifers. However, conifers have an advantage over non-coniferous plantlets with respect to water loss because of a better development of the needle cuticles prior to transfer to in vivo conditions. In greenhouse and field comparisons with seedlings, plantlets were similar in survival and growth rate, but root systems were less fibrous. Also, features of early maturation have been observed for plantlets, the cause of which is uncertain. Pertinent research with rooted cuttings and seedlings of conifers has been cited to gain a better understanding of the factors involved in root production and development.

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In vitro rooting of cloned shoots in Pinus pinaster

Cited by 25 publications

References 10 publications

Artefactual Origins of Cyclic AMP in Higher Plant Tissues

Artefactual Origins of Cyclic AMP in Higher Plant Tissues

Micropropagation of the pine hybrid Pinus brutia (Ten) × Pinus halepensis (Mill) by culturing fascicle shoots

Root production and plantlet development in tissue-cultured conifers

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