In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights

Leite, Cristiana Torres; Ferreira, Darley Aparecido Tavares; Vieira, Ariane Tonetto; Fontes, Milene Miranda Praça; Ferreira, Adésio; Carvalho, Carlos Roberto

doi:10.1007/s11240-018-01536-9

Cited by 4 publications

(9 citation statements)

References 33 publications

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“…Thus, the expression corresponds to plant regeneration frequency. These results are in accordance with the previous describing that, OsLEC1 expression was found to be increased in varieties with a high rate of plant regeneration in this study, these results are in accordance with the previous describing that, OsLEC1 gene expressed during somatic embryogenesis maturation in Passiflora species (Leite et al, 2019). Other investigations discovered that the LEC1 gene is a critical regulator of somatic embryogenesis in Arabidopsis thaliana and plays a critical role in somatic embryogenesis (Gaj et al, 2005).…”

Section: Discussionsupporting

confidence: 94%

Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development

NABILAH¹,

Handoyo²,

KIM³

et al. 2022

Biocell

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Somatic embryogenesis is an asexual reproduction process that occurs in many plant species, including rice. This process contains several totipotency markers such as Somatic Embryogenesis Receptor-like Kinase (SERK), Leafy Cotyledon1 (LEC1) and WUSCHEL-Related Homeobox4 (WOX4) and also a helpful model for embryo development and clones and transformations. Here, we report the gene expression during somatic embryo development correlates with regeneration frequency in 14 Javanica rice (pigmented and non-pigmented) using modified N6 media supplemented with Kinetin (2.0 mg/L) and NAA (1.0 mg/L). Although there have been advances in understanding the genetic basis of somatic embryogenesis in other varieties, rice is still unexplored, especially during somatic embryo development. Moreover, for the formation of callus induction from immature embryos, 2,4-D (2.0 mg/L, 3.0 mg/L) was used. This study analysed the gene expression of OsSERK, OsWOX4 and OsLEC1 genes through RT-PCR analysis. Higher expression of the OsLEC1 gene indicates that their function may correlate in the in vitro with the high response of rice after transfer to regeneration media. This study found that rice varieties of pigmented rice (MS Pendek and Gogoniti II) and non-pigmented rice (Pandan Ungu) showed high regeneration frequency, showing higher OsLEC1 expression than other varieties because OsLEC1 promotes the maturation of somatic embryos in plant regeneration on day 14. However, the contrast with Genjah nganjuk may be effective because of other regulatory genes. RT-PCR analysis showed OsSERK had less expression level than OsLEC1 and OsWOX4 in the varieties, which correlate with the percentage of plant regeneration, but not for Gogoniti II. In conclusion, the higher percentage of plant regeneration correlates with the higher expression level of OsLEC1 at day 14 of media regeneration of rice.

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Section: Discussionsupporting

confidence: 94%

Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development

NABILAH¹,

Handoyo²,

KIM³

et al. 2022

Biocell

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“…Producing clones is a most censorious factors due to somaclonal variation that arises as a direct consequences of plant cell and tissues cultured in vitro. In recent years, flow cytometry has been widely used for the estimation of DNA and ploidy levels of in vitro plants and it appears to be an efficient and reliable method for characterization in the regenerated plants (Obae and West, 2010, Vujović et al, 2012, Faisal et al, 2014, Faisal et al, 2018, Leite et al, 2019). In this study, the microprogramed plants derived from the axillary buds were verified by flow cytometry and no differences in ploidy were recorded, as it was clearly evident in the histogram generated by flow cytometric analysis of the nuclei isolated from both the tissue culture as well as ex vitro grown plants (Fig.…”

Section: Resultsmentioning

confidence: 99%

Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. ‘White Butterfly’

Faisal

Alatar

Qahtan

2020

Saudi Journal of Biological Sciences

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There are more than nine thousand cultivars of Hibiscus rosa-sinensis L., with a series of flowers with shapes, colors and new cultivars continues as generated through both traditional and modern breeding techniques. In this study, advanced biotech methods of in vitro culture have been used to identify a technique for the efficient mass multiplication of H. rosa-sinensis ‘White Butterfly’, using phenyl urea, N-(2-Chloro-4-pyridyl)-N′-phenylurea (4-CPPU). For the first time, the effects of 4-CPPU for stimulating axillary shoot proliferation and multiple shoot regenerations from nodal explants were evaluated, and the optimal nutrient media deduced. From the diverse concentrations as 0.1, 0.5, 2.5, 5.0 & 10.0 µM of 4-CPPU, the highest frequency of shoots was recorded at 2.5 µM supplied in Murashige and Skoog (MS, pH-5.8) medium. After eight-weeks of culture, on an average of 6.7 shoot were obtained on this media with shoot heights of 4.2 cm from each explant. With the involvement of 0.5 µM-IBA (indole-3-butyric acid) in MS medium the regenerated shoots were rooted and followed by successful acclimation to ex vitro conditions. The ploidy consistency among the micro-plants was analyzed using flow cytometry and compared with ex vitro grown plants. No differences in the ploidy levels were observed among the 4-CPPU induced plants, when compared with the donor plants.

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“…Studies focusing on the chromosomes of the genus Passiflora have found different basic chromosome numbers: x= 6 (2n=12, 24 and 36) in the subgenus Decaloba, x= 9 (2n=18, 36 and 72), x=10 (2n=20) in the subgenus Passiflora, and x=12 (2n=24) in subgenera Astrophea Guerra 2003, Leite et al 2019), Deidamioides (Hansen et al 2006, Leite et al 2019 and Tetrapathea (Hansen et al 2006). The x=9, 10 and 12 are considered secondary basic numbers (Melo et al 2001(Melo et al , 2014.…”

mentioning

confidence: 99%

“…The x=9, 10 and 12 are considered secondary basic numbers (Melo et al 2001(Melo et al , 2014. In addition, intra-and interspecific variations in the chromosome number have been found, for example in P. suberosa L. (2n=2x=12, 2n=4x=24 and 2n=6x=36) or in P. misera Kunth (2n=2x=12 and 2n=6x=36, Melo et al 2001), P. foetida L. and P. sublanceolata (Killip) MacDougal (2n=2x=22, Santos et al 2012), and P. contracta Vitta (2n=8x= 48, Leite et al 2019). Therefore, it is indispensable to combine techniques that expand the karyotype description for comprehensive cytogenetic research of Passiflora.…”

mentioning

confidence: 99%

“…Porter-Utley (2014) reported that there are little variations for some descriptors among the three species P. megacoriacea, P. coriacea, and P. sexocellata. From this group, only the morphometric karyotype features of P. coriacea have already been described: it shows 2n=2x=12 chromosomes (Leite et al 2019, Sader et al 2019, with chromosome 2 being metacentric and the others submetacentric (Leite et al 2019). Considering this problem, it is necessary to incorporate more informative techniques that allow a broad mode of analysis for a better taxonomic delimitation between these species.…”

mentioning

confidence: 99%

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Cytogenetic Characterization of <i>Passiflora megacoriacea</i> K.Port.-Utl. Employing Image Cytometry

et al. 2019

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The Passiflora coriacea complex encompasses the species P. megacoriacea K.Port.-Utl., P. coriacea Juss and P. sexocellata Schltdl, and the morphological similarities among the species have posed challenges for taxonomic and phylogenetic studies in the past. Image cytometry (ICM) has been used to improve the karyotype characterization in some plant species and has allowed the identification of small variations in the DNA content between homomorphic chromosomes. The aim of the present study was to determine the chromosome number, characterize the karyotype and measure the chromosomal DNA content in P. megacoriacea using classical cytogenetic and ICM approaches. We observed a chromosome number of 2n=12, with the chromosomes 1-3 and 5 classified as submetacentric and chromosomes 4 and 6 as metacentric in all metaphase plates. The nuclear DNA content (1C= 0.51 pg) was measured by flow cytometry and then distributed proportionally to mean integrated optical density values calculated by ICM from Feulgen stained chromosomes. So, the mean 1C chromosomal DNA content varied of 0.129 pg for chromosome 1 to 0.054 pg for chromosome 6. We can thus establish that the ensemble analysis of qualitative and quantitative parameters allowed us the first karyotype characterization of P. megacoriacea, providing data to a better taxonomic delimitation among the species of the P. coriacea Juss complex.

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In vitro responses in Passiflora species with different chromosome numbers, ploidy levels and nuclear 2C values: revisiting and providing new insights

Cited by 4 publications

References 33 publications

Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development

Expression analysis of OsSERK, OsLEC1 and OsWOX4 genes in rice (Oryza sativa L.) callus during somatic embryo development

Effects of 4-CPPU on in vitro multiplication and sustainable generation of Hibiscus rosa-sinensis L. ‘White Butterfly’

Cytogenetic Characterization of <i>Passiflora megacoriacea</i> K.Port.-Utl. Employing Image Cytometry

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