In vitro response of macrophage polarization to a keratin biomaterial

Fearing, Bailey V.; Dyke, Mark Van

doi:10.1016/j.actbio.2014.04.003

Cited by 92 publications

(68 citation statements)

References 65 publications

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“…However, Mφ on cl_CM interestingly tended to polarize toward the M1phenotype and showed higher mRNA levels of IL-1 and IL-6 and CD86 cytokine expression, while an unclear polarization profile was observed in ncl_CM. This is consistent with previous reports that Mφ cultured on the collagen coating surface-expressed mostly M1 surface markers (CD86) but also some M2 markers and produced high levels of proinflammatory cytokines(Fearing & Van Dyke, 2014). Furthermore, cross-linking of collagen is associated with Mφ polarization profiles, as indicated by the residual chemical cross-links in the material(Delgado, Bayon, Pandit, & Zeugolis, 2015).…”

supporting

confidence: 93%

In vitro observation of macrophage polarization and gingival fibroblast behavior on three‐dimensional xenogeneic collagen matrixes

Fujioka‐Kobayashi

Ülgür

Katagiri

et al. 2020

J Biomedical Materials Res

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Collagen biomaterials are widely used for soft tissue augmentation. Cross‐linking techniques for collagen matrix (CM) achieve mechanical and volumetric stability; nevertheless, cross‐linking may compromise biocompatibility. The aim of the present study was to investigate two different three‐dimensional (3D) porcine‐derived CMs, noncross‐linked (ncl)_CM and cross‐linked (cl)_CM, for their effects on macrophages (Mφ) and gingival fibroblasts. The effects of the CMs on the cell viability, proliferation, and polarization of Mφ derived from human monocyte THP‐1 cells were assessed. The effects of paracrine factors from Mφ cultured on the CMs were further studied in human gingival fibroblasts (HGF‐1 cells). The spongy layer of ncl_CM was partially resorbed after 1 day of culture. cl_CM maintained increased numbers of viable cells when compared with ncl_CM on day 3 for both THP‐1 and HGF‐1 cells. Higher mRNA levels of M1 markers, including IL‐1 and IL‐6, were found in Mφ cultured on cl_CM, while no significant differences were observed in M2 marker expression levels, including Arg1 and CD206, for cells cultured on both CMs when compared with those of the control. Furthermore, the conditioned medium collected from Mφ cultured on both CMs decreased cell viability. Nevertheless, neither of the CM‐conditioned media influenced the mRNA levels of TGF‐β, COL1a2, and PDGF‐A in HGF‐1 cells when compared with the control media. A comparison showed that cl_CM tended to result in more viable cells than ncl_CM, while cl_CM polarized Mφ toward an M1 phenotype, which was confirmed by the observation of increased mRNA levels of pro‐inflammatory cytokines.

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supporting

confidence: 93%

In vitro observation of macrophage polarization and gingival fibroblast behavior on three‐dimensional xenogeneic collagen matrixes

Fujioka‐Kobayashi

Ülgür

Katagiri

et al. 2020

J Biomedical Materials Res

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“…Other groups have shown that macrophages exposed to keratin proteins produced by crude extraction processes, preferentially differentiate to the M2 anti-inflammatory and reparative phenotype. 41 Though it is tempting to make the argument that the increases of macrophages in ciprofloxacin-loaded keratose hydrogels are due to an increase in M2 macrophages based on this study, there were no increases in macrophages seen in the keratose only-treated wounds. This suggests that the macrophage increase in the ciprofloxacin-loaded keratose hydrogels is unrelated to the keratin used in this study, and it is likely due to the presence of ciprofloxacin in these wounds.…”

Section: Discussionmentioning

confidence: 63%

Ciprofloxacin-Loaded Keratin Hydrogels PreventPseudomonas aeruginosaInfection and Support Healing in a Porcine Full-Thickness Excisional Wound

Roy

Tomblyn

Burmeister

et al. 2015

Advances in Wound Care

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Objective: Cutaneous wound infection can lead to impaired healing, multiple surgical procedures, and increased hospitalization time. We tested the effectiveness of keratin-based hydrogels (termed ''keratose'') loaded with ciprofloxacin to inhibit infection and support healing when topically administered to porcine excision wounds infected with Pseudomonas aeruginosa. Approach: Using a porcine excisional wound model, 10 mm full-thickness wounds were inoculated with 10 6 colony-forming units of P. aeruginosa and treated on days 1 and 3 postinoculation with ciprofloxacin-loaded keratose hydrogels. Bacteria enumeration and wound healing were assessed on days 3, 7, and 11 postinjury. Results: Ciprofloxacin-loaded keratose hydrogels reduced the amount of P. aeruginosa in the wound bed by 99.9% compared with untreated wounds on days 3, 7, and 11 postinjury. Ciprofloxacin-loaded keratose hydrogels displayed decreased wound contraction and reepithelialization at day 7 postinjury. By day 11, wounds treated with ciprofloxacin-keratose hydrogels contained collagen-rich granulation tissue and myofibroblasts. Wounds treated with ciprofloxacin-loaded keratose hydrogels exhibited a transient increase in macrophages in the wound bed at day 7 postinjury that subsided by day 11. Innovation: Current therapies for wound infection include systemic antibiotics, which could lead to antibiotic resistance, and topical antimicrobial treatments, which require multiple applications and can delay healing. Here, we show that ciprofloxacin-loaded keratose hydrogels inhibit cutaneous wound infection without interfering with key aspects of the healing process including granulation tissue deposition and remodeling. Conclusions: Ciprofloxacin-loaded keratose hydrogels have the potential to serve as a point-of-injury antibiotic therapy that prevents infection and supports healing following cutaneous injury.

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“…The two distinct states of Mφs elicit divergent effects: the M1 phenotype cells upregulate activities related to tissue destruction via secretion of numerous proinflammatory cytokines, such as tumor necrosis factor alpha (TNFα), interleukin-1β (IL-1β), interleukin-12 (IL-12) and interleukin-6 (IL-6); whereas cells with an M2 subtype support tissue remodeling by releasing large amounts of anti-inflammatory cytokines, such as interleukin-10 (IL-10) and transforming growth factor beta (TGFβ) [18, 19]. Therefore, Mφs can promote both positive and negative outcomes depending on context-dependent polarization profiles, and transition from an M1 phenotype to an M2 phenotype is a key strategy to control and promote tissue regeneration [20, 21]. Although concerted efforts have been placed into using biomaterials to coax Mφ polarization, investigations into interactions between MSCs and Mφs also substantiate the fact that functional MSCs can drive protective M2 polarization [22–27].…”

Section: Introductionmentioning

confidence: 99%

Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture

Yin

et al. 2017

Stem Cell Res Ther

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In vitro response of macrophage polarization to a keratin biomaterial

Cited by 92 publications

References 65 publications

In vitro observation of macrophage polarization and gingival fibroblast behavior on three‐dimensional xenogeneic collagen matrixes

In vitro observation of macrophage polarization and gingival fibroblast behavior on three‐dimensional xenogeneic collagen matrixes

Ciprofloxacin-Loaded Keratin Hydrogels PreventPseudomonas aeruginosaInfection and Support Healing in a Porcine Full-Thickness Excisional Wound

Influences of age-related changes in mesenchymal stem cells on macrophages during in-vitro culture

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