In vitro response of Indica rice microspores subjected to cold stress: a cytological and histological perspective

Mayakaduwa, D. M. R. G.; Silva, T. D.

doi:10.1007/s11627-021-10177-1

Cited by 5 publications

(20 citation statements)

References 33 publications

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“…However, some bottlenecks mitigate the widespread utilization of in vitro anther culture in rice breeding, especially in indica genotypes. Recently, several research groups have focused on the further improvement of AC in rice [6][7][8][9][10][11][12][13][14][15]. The first AC-derived green plantlet production was published in 1968 by Niizeki and Oono [16]; since then, significant improvements have been achieved by different research groups to improve the efficiency of AC [17][18][19][20].…”

Section: Introductionmentioning

confidence: 99%

“…Several factors such as genotype, growing conditions of donor plants, developmental stage of microspores, pre-treatments, and compositions of the induction and plant regeneration media influence the efficiency of in vitro AC. Furthermore, genotype dependency, low induction rate, plant regeneration rate and albinism limit the high frequency of doubled haploid (DH) plant production in rice, especially in indica genotypes [6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…Among Oryza species and subspecies, the efficiency of AC is significantly influenced by the genotype particularly among the indica genotypes, as lines and varieties are more recalcitrant in AC than in Oryza sativa ssp. japonica genotypes [6,13,[21][22][23][24][25][26][27][28][29]. The necrosis of anthers leading to cell death, low frequency of callus induction, low plant regeneration efficiency and the high proportion of albinos are the most critical limiting factors for AC of indica genotypes [6,8,14,15,19,21].…”

Section: Introductionmentioning

confidence: 99%

“…influence the efficiency of AC. In the indica rice, the most frequently applied basal medium was the N 6 medium [13,[30][31][32], but some other media (Ali-1, CIM) were also used for the induction of in vitro androgenesis in AC [28,29,33]. In the induction of androgenesis in rice, maltose is the most frequently applied carbohydrate source due to its positive effect on increasing callus induction and green plantlet regeneration [12,13,20,21].…”

Section: Introductionmentioning

confidence: 99%

“…In the indica rice, the most frequently applied basal medium was the N 6 medium [13,[30][31][32], but some other media (Ali-1, CIM) were also used for the induction of in vitro androgenesis in AC [28,29,33]. In the induction of androgenesis in rice, maltose is the most frequently applied carbohydrate source due to its positive effect on increasing callus induction and green plantlet regeneration [12,13,20,21]. Exogenous growth regulators, such as 1-naphtylacetic acid (NAA), 6-benzylaminopurin (BAP), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin, zeatin and their combinations, were applied for androgenesis induction of indica rice genotypes [13,23,[28][29][30]32,33].…”

Section: Introductionmentioning

confidence: 99%

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Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Lantos

Jancsó

Székely

et al. 2023

Plants

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Anther culture is an efficient biotechnological tool in modern plant breeding programs to produce new varieties and parental lines in hybrid seed productions. However, some bottlenecks—low induction rate, genotype dependency, albinism—restrict the widespread utilization of in vitro anther culture in rice breeding, especially in Oryza sativa ssp. indica (indica) genotypes, while an improved efficient protocol can shorten the process of breeding. Three different induction media (N6NDK, N6NDZ, Ali-1) and four plant regeneration media (mMSNBK1, MSNBK3, MSNBKZ1, MSNBKZ2) were tested with five indica rice genotypes to increase the efficiency of in vitro androgenesis (number of calli and regenerated green plantlets). The production of calli was more efficient on the N6NDK medium with an average 88.26 calli/100 anthers and N6NDZ medium with an average of 103.88 calli/100 anthers as compared to Ali-1 with an average of 6.96 calli/100 anthers. The production of green plantlets was greater when calli was produced on N6NDK medium (2.15 green plantlets/100 anthers) compared when produced on to N6NDZ medium (1.18 green plantlets/100 anthers). Highest green plantlets production (4.7 green plantlets/100 anthers) was achieved when mMSNBK1 plant regeneration medium was used on calli produced utilizing N6NDK induction medium. In the best overall treatment (N6NDK induction medium and mMSNBK1 plant regeneration medium), four tested genotypes produced green plantlets. However, the genotype influenced the efficiency, and the green plantlets production ranged from 0.4 green plantlets/100 anthers to 8.4 green plantlets/100 anthers. The ploidy level of 106 acclimatized indica rice plantlets were characterized with flow cytometric analyses to calculate the percentage of spontaneous chromosome doubling. Altogether, 48 haploid-, 55 diploid-, 2 tetraploid- and 1 mixoploid plantlets were identified among the regenerant plantlets, and the spontaneous chromosome doubling percentage was 51.89%. Utilization of DH plants have been integrated as a routine method in the Hungarian rice breeding program. The tetraploid lines can be explored for their potential to offer new scopes for rice research and breeding directions in the future.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Lantos

Jancsó

Székely

et al. 2023

Plants

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In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino

Dang

Gao

Zhang

et al. 2022

Sci Rep

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Dioscorea nipponica Makino is an optimal candidate to develop the diosgenin industry in North China. Due to its increasing demand in the medicine industry, it is urgent to apply new biotechnological tools to foster breeds with desirable traits and enhanced secondary metabolite production. The production of useful metabolites by the in vitro cultured rhizomes can be explored successfully for utilization by various food and drug industries. In this study, we reported callus formation and plantlet regeneration of the medicinal plant D. nipponica. Explants of leaves, stem segments and rhizomes of aseptic seedlings were cultured on Murashige and Skoog (MS) medium containing various combinations of auxin and cytokinin to find the optimal PGRs of each type of explant for callus induction and shoot regeneration of D. nipponica. The paraffin section technique was also used to observe of the morphogenesis of callus and adventitious bud. Explants of seeds and rhizomes formed calli at high frequency in all lines we examined. However, the explant of leaves rarely formed callus. Three kinds of callus were detected during the induction phase. Here, we describe three types of callus (Callus I–III) with different structure characteristics. Greenish in color and a nodule-like protrusion surface (Callus type III) were arranged more closely of cells with less interstitial substance, cell differentiation ability stronger than other callus types. The optimum combination was the maximum shoot differentiation frequency of 90% in callus derived from seeds cultured on MS medium with 2.0 mg L−16-BA + 0.2 mg L−1NAA. The shoot differentiation frequency (88.57%) of rhizome-induced callus was obtained by the combination of MS medium supplemented with 3.0 mg L−16-BA + 2.0 mg L−1NAA. 1/2 MS medium plus 0.5 mg L−1NAA resulted in a higher root regeneration frequency of 86.70%. In vitro propagated plantlets with healthy roots were domesticated and transplanted into small plastic pots containing sterile soil rite under greenhouse conditions with 80% survivability. Bud differentiation is mostly of exogenous origin, mostly occurring on the near callus surface. Therefore, it may be surmised that in vitro morphogenesis of D. nipponica is mainly caused by indirect organogenesis (adventitious bud).

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Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rice (Oryza sativa L.) Breeding

Lantos

Jancsó

Székely

et al. 2022

Plants

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Doubled haploid (DH) plant production, such as anther culture (AC), is an effective tool used in modern rice breeding programs. The improved efficient protocols applied can shorten the process of breeding. The effect of combinations of plant growth regulators (2.5 mg/L NAA, 1 mg/L 2,4-D and 0.5 mg/L kinetin; 2 mg/L 2,4-D and 0.5 mg/L BAP) in the induction medium were compared in AC for five rice breeding materials and combinations. Induction of calli ranged from 264.6 ± 67.07 to 468.8 ± 123.2 calli/100 anthers in AC of rice genotypes. Two basal media (MS and N6) and two combinations of growth regulators (1 mg/L NAA, 1 mg/L BAP and 1 mg/L kinetin; 1.5 mg/L BAP, 0.5 mg/L NAA and 0.5 mg/L kinetin) were used as regeneration media. The in vitro green plant production was the highest with the application of the N6NDK induction medium (NAA, 2,4-D and kinetin) and the MS-based regeneration medium (1 mg/L NAA, 1 mg/BAP and 1 mg/L kinetin) in anther culture of the ‘1009’ genotype (95.2 green plantlets/100 anthers). The mean of five genotypes was 24.48 green plantlets/100 anthers for the best treatment. Flow cytometric analyses conducted identified the microspore origin of the haploid calli produced in AC, while the uniformity of spontaneous DH plants was checked in the DH1 and DH2 generations. Spontaneous chromosome doubling ranged from 38.1% to 57.9% (mean 42.1%), depending on the breeding source. The generated and selected DH lines were tested in micro- and small-plot field experiments to identify promising lines for a pedigree breeding program. The improved AC method was integrated in a Hungarian temperate rice pedigree breeding program.

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In vitro response of Indica rice microspores subjected to cold stress: a cytological and histological perspective

Cited by 5 publications

References 33 publications

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

Development of In Vitro Anther Culture for Doubled Haploid Plant Production in Indica Rice (Oryza sativa L.) Genotypes

In vitro regeneration and its histological characteristics of Dioscorea nipponica Makino

Improvement of Anther Culture to integrate Doubled Haploid Technology in Temperate Rice (Oryza sativa L.) Breeding

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