In vitro reproduction of rare and endemic species of rowan tree

Máchová, P.; Malá, Jana; Cvrčková, H.; Dostál, J.; Buriánek, V.

doi:10.17221/46/2013-jfs

Cited by 6 publications

(6 citation statements)

References 8 publications

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“…In vitro rooting of microcuttings has been reported for many Sorbus species and their infrageneric hybrids: S. domestica [ 34 , 37 ], S. aucuparia [ 43 ], S. torminalis [ 30 ], S. commixta [ 58 ], × Malosorbus florentina (Zuccagni) Browicz [ 41 ] and also seven rare and endemic Sorbus species [ 42 ]. By contrast, ex vitro rooting has been reported only from an experiment with S. torminalis , where root induction took place in vitro and expression occurred under greenhouse conditions [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

“…The use of various auxins for in vitro root formation in Sorbus species has been reported as follows: Rhizogenesis was induced with IBA in S. commixta and in S. × abscondita [ 58 , 79 ]; in S. domestica , a high percentage of rooting was achieved with both IBA and NAA. A combination of IBA with NAA proved effective in S. torminalis and S. aucuparia [ 32 , 43 ], and a combination of IBA with IAA was found to have an effect in infrageneric hybrid × Malosorbus florentina [ 41 ] and NAA has been used with success in seven rare endemic species [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

“…rhodanthera and S . sudetica [ 42 ]. These species are important primarily from an ecological point of view, as they increase the biodiversity of woodland ecosystems.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

Šedivá

Velebil

Zahradník

2023

Plants

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Members of the genus Sorbus are the only endemic tree species that occur in Czechia. They are important components of endangered plant communities. Their natural regeneration is usually problematic because of their mode of reproduction and because they can survive in rare populations with small numbers of individuals. The aim of this study was to develop a successful micropropagation protocol for selected Sorbus species, of which two are endemic (S. gemella and S. omissa) and two are hybrid (S. × abscondita and S. × kitaibeliana). We found significant differences in shoot induction and rooting ability between the Sorbus species under study. With the exception of S. × abscondita, N6-benzyladenine had a significantly greater effect on shoot regeneration, both in terms of shoot number and total shoot length, than meta-topolin. Root induction was key to the successful micropropagation of the Sorbus species studied. Our results show that four Sorbus species can be successfully rooted under ex vitro conditions, without a rooting powder treatment in a steamed peat-perlite substrate. Auxin-untreated microcuttings of S. gemella, S. × kitaibeliana and S. omissa, but not S. × abscondita, rooted better than ones treated with indole-3-butyric acid. This is the first time a micropropagation protocol for S. omissa, S. × abscondita and S. × kitaibeliana has been published.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

Šedivá

Velebil

Zahradník

2023

Plants

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“…It provides the large-scale production of disease-free seedlings within a short time and with limited space. In the Czech Republic, biotechnology approaches for in vitro conservation of different woody plant species are well established and widely used (e.g., see [12][13][14][15][16][17]). During the past years, many studies have been carried out using in vitro cultures of apples (for review, see [18]).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Observation of In Vitro-Derived Malus Sylvestris (L.) Mill., the Path from the Bud to the Tree

Komárková,

Cvrčková,

Dostál

et al. 2023

Apple Cultivation - Recent Advances

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The European wild apple (Malus sylvestris L.), a wild contributor to the domesticated apple, belongs to the endangered species in the Czech Republic. Thus, an efficient protocol was developed for in vitro plantlet regeneration using the post-dormant buds. The highest shoot induction was obtained on MS medium supplemented with 0.5 mg.l−1 BAP, 5 mg.l−1 GA and 0.1 mg.l−1 IBA. Shoot multiplication and elongation took place on the same medium with 0.2 mg.l−1 BAP and 0.1 mg.l−1 IBA. Indole-3-butyric acid at 0.5 mg.l−1 was most effective for rooting. The micropropagated plantlets were successfully acclimatized in greenhouse conditions and were transplanted into soil in forest. Finally, qualitative and quantitative parameters of tissue culture-derived plants were evaluated. Monitoring of in vitro plantings on experimental trials suggests that micropropagated wild apple trees retain the growth characteristics of generative individuals.

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“…Chalupa 1979;Malá et al 2012Malá et al , 2013Malá et al , 2014Máchová et al 2013). Micropropagation can produce high-quality material for standard planting as well as for establishing clonal archives and seed orchards, repatriation, storage in a tissue culture bank, monitoring of genetic variability, and so forth.…”

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confidence: 99%

Comparison of growth development of micropropagated and generatively reproduced wild cherry (Prunus avium (L.) L.) on the Polná demonstration plot (Czech Republic) up to the age of 15 years

Dostál¹,

Novotný²,

Čáp³

2016

J. For. Sci.

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Four clonal progenies of wild cherry obtained using an in vitro technique and control progeny of generative origin were tested on the Polná research plot in southeastern Bohemia. Growth characteristics of cherry trees were determined each year during the period 2000-2010. At the age of 15 years, the progenies reach mean heights of 4-9 m and diameter at breast height (DBH) of 5-10.5 cm. The determined differences in height and DBH between progenies are statistically significant. Cherry trees of generative origin and progeny of clone No. 14 grow markedly slower in comparison with clones Nos 24, 26 and 28. The values of growth characteristics for verified clones are comparable with similar findings from abroad. The in vitro plantlets of wild cherry achieve similar growth characteristics as generative seedlings.

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In vitro reproduction of rare and endemic species of rowan tree

Abstract: ABSTRACT:The preservation and reproduction of gene resources of rare and endemic rowan species were the main aims of this study. Rowan species represent important woody trees from the aspect of forest biodiversity.

Cited by 6 publications

References 8 publications

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

Micropropagation as a Tool for the Conservation of Autochthonous Sorbus Species of Czechia

Long-Term Observation of In Vitro-Derived Malus Sylvestris (L.) Mill., the Path from the Bud to the Tree

Comparison of growth development of micropropagated and generatively reproduced wild cherry (Prunus avium (L.) L.) on the Polná demonstration plot (Czech Republic) up to the age of 15 years

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