In vitro replication and expression of hepatitis B virus from chronically infected primary chimpanzee hepatocytes

Jacob, James R.; Eichberg, Jorg W.; Lanford, Robert E.

doi:10.1002/hep.1840100605

Cited by 19 publications

(11 citation statements)

References 29 publications

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“…The culture system used in this work was based on the one described previously for baboon and chimpanzee primary hepatocyte cultures Jacob et al, 1989). Slight modifications have been introduced, however, to take into account the specificity of human hepatocytes.…”

Section: Cell Culture Conditionsmentioning

confidence: 99%

In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus.

Fournier

Sureau

Coste

et al. 1998

Journal of General Virology

120

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In vitro infection of adult normal human hepatocytes in primary culture has been performed for investigating the replication cycle of hepatitis C virus (HCV) in differentiated cells. Hepatocytes were prepared from liver tissue resected from donors who tested negative for HCV, and inoculation was performed 3 days after plating with 33 HCV serum samples of different virus load and genotype. The presence of intracellular HCV RNA, detected by a strand-specific rTth RT-PCR assay, was used as evidence of infection. A kinetics analysis of HCV replication revealed that intracellular negative-strand RNA appeared at day 1 post-infection with a maximum level at days 3 and 5, followed by a decrease until

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Section: Cell Culture Conditionsmentioning

confidence: 99%

In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus.

Fournier

Sureau

Coste

et al. 1998

Journal of General Virology

120

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“…Hepatocytes were allowed to attach for 3 h in Williams medium E supplemented with 5% fetal bovine serum, at which time the cultures were washed and changed to a serum-free medium supplemented with growth factors and hormones (10). The cultivation and characterization of baboon and chimpanzee hepatocytes in serum-free medium have been described (7,8,10). Secretion of apolipoproteins Al and E by the hepatocyte cultures was monitored by Western immunoblot as stringent markers of differentiation.…”

Section: Methodsmentioning

confidence: 99%

“…In the studies described here, we set out to cultivate CHV in primary cultures of marmoset hepatocytes (10) because hepatocytes are one of the targets of CHV infection in vivo * Corresponding author. and in vitro cultures of these cells are known to be permissive for the replication of other hepatotropic viruses (7,8). Our initial goal was to demonstrate replication of CHV in cell culture by using CH-specific antisera from convalescent animals (17).…”

mentioning

confidence: 99%

Isolation of an arenavirus from a marmoset with callitrichid hepatitis and its serologic association with disease

Stephensen

Jacob

Montali

et al. 1991

J Virol

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Callitrichid hepatitis (CH) is an acute, often fatal viral infection of New World primates from the family Callitrichidae. The etiologic agent of CH is unknown. We report here the isolation of an arenavirus from a common marmoset (Callithrir jacchus) with CH by using in vitro cultures of marmoset hepatocytes and Vero-E6 cells. Enveloped virions 67 to 133 nm in diameter with ribosomelike internal structures were seen in infected cultures. Immunofluorescence and Western immunoblot analysis using CH-specific antisera (principally from animals exposed to CH during zoo outbreaks) revealed three antigens in cells infected with this CH-associated virus (CHV). These antigens had the same electrophoretic mobilities on sodium dodecyl sulfate-polyacrylamide gels as did the nucleocapsid, GP2, and GPC proteins of lymphocytic choriomeningitis virus (LCMV). Monoclonal antibodies specific for these arenavirus proteins also reacted with the three CHV antigens. Conversely, the CH-specific antisera reacted with the nucleocapsid, GP2, and GPC proteins of LCMV. CHV thus appears to be a close antigenic relative of LCMV. The serologic association of CHV with several CH outbreaks implicate it as the etiologic agent of this disease.

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“…These animals were free of any HDV markers including HDV RNA and anti-HDAg antibodies in the serum and had no history of HDV infection. The procedures utilized for the isolation and culture of primary hepatocytes in a serumfree medium formulation have been described previously (15).…”

mentioning

confidence: 99%

Role of the large hepatitis B virus envelope protein in infectivity of the hepatitis delta virion

Sureau

Guerra

Lanford

1993

J Virol

132

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The hepatitis delta virus (HDV) is coated with large (L), middle (M), and small (S) envelope proteins encoded by coinfecting hepatitis B virus (HBV). To study the role of the HBV envelope proteins in the assembly and infectivity of HDV, we produced three types of recombinant particles in Huh7 cells by transfection with HBV DNA and HDV cDNA: (i) particles with an envelope containing the S HBV envelope protein only, (ii) particles with an envelope containing S and M proteins, and (iii) particles with an envelope containing S, M, and L proteins. Although the resulting S-, SM-, and SML-HDV particles contained both hepatitis delta antigen and HDV RNA, only particles coated with all three envelope proteins (SML) showed evidence of infectivity in an in vitro culture system susceptible to HDV infection. We concluded that the L HBV envelope protein, and more specifically the pre-Sl domain, is important for infectivity of HDV particles and that the M protein, which has been reported to bear a site for binding to polymerized albumin in the pre-S2 domain, is not sufficient for infectivity. Our data also show that the helper HBV is not required for initiation of HDV infection. The mechanism by which the L protein may affect HDV infectivity is discussed herein.

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In vitro replication and expression of hepatitis B virus from chronically infected primary chimpanzee hepatocytes

Cited by 19 publications

References 29 publications

In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus.

In vitro infection of adult normal human hepatocytes in primary culture by hepatitis C virus.

Isolation of an arenavirus from a marmoset with callitrichid hepatitis and its serologic association with disease

Role of the large hepatitis B virus envelope protein in infectivity of the hepatitis delta virion

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