In vitro repair of radiation-induced strand breaks in DNA

Mitzel-Landbeck, Luitgard; Schütz, Gisela; Hagen, U.

doi:10.1016/0005-2787(76)90156-8

Cited by 32 publications

(13 citation statements)

References 16 publications

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“…It will remove other 3′ residues damaged by radiation or oxidation, such as 3′ phosphoglycolate, 3′ phosphoaldehyde and urea N-glycosides (68, 106, 138, 183, 193). Exonuclease III also possesses endonuclease activity on apurinic/apyrimidinic DNA (265), an activity originally purified as “endonuclease II”.…”

Section: E Coli Exonucleases: Properties Structure and Functionmentioning

confidence: 99%

The DNA Exonucleases of Escherichia coli

Lovett

2011

EcoSal Plus

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DNA exonucleases, enzymes that hydrolyze phosphodiester bonds in DNA from a free end, play important cellular roles in DNA repair, genetic recombination and mutation avoidance in all organisms. This article reviews the structure, biochemistry and biological functions of the 17 exonucleases currently identified in the bacterium Escherichia coli. These include the exonucleases associated with DNA polymerases I (polA), II (polB) and III (dnaQ/mutD), Exonucleases I (xonA/sbcB), III (xthA), IV, VII (xseAB), IX (xni/xgdG) and X (exoX), the RecBCD, RecJ, and RecE exonucleases, SbcCD endo/exonuclease, the DNA exonuclease activities of RNase T (rnt) and Endonuclease IV (nfo) and TatD. These enzymes are diverse in terms of substrate specificity and biochemical properties and have specialized biological roles. Most of these enzymes fall into structural families with characteristic sequence motifs, and members of many of these families can be found in all domains of life.

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Section: E Coli Exonucleases: Properties Structure and Functionmentioning

confidence: 99%

The DNA Exonucleases of Escherichia coli

Lovett

2011

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“…For example, both H202 and y-rays form relatively large numbers ofDNA strand breaks (1,5). The breaks produced by ionizing radiation do not provide substrates in vitro for Escherichia coli DNA polymerase I or DNA ligase (6,7), and so apparently contain blocked 3' termini. The breaks produced by H202 have not been examined previously for this property.…”

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confidence: 99%

Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli.

Demple

Johnson

Fung

1986

Proc. Natl. Acad. Sci. U.S.A.

246

159

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“…In a previous communication (Mitzel-Landbeck et al, 1976) it was shown that a successfull repair of strand breaks with damaged end groups is possible only after their removal by exonucleases. In a previous communication (Mitzel-Landbeck et al, 1976) it was shown that a successfull repair of strand breaks with damaged end groups is possible only after their removal by exonucleases.…”

Section: Discussionmentioning

confidence: 99%

End group characterization in DNA of thymocytes after low doses of ionizing radiation

Coquerelle

Sexauer

1977

Radiat Environ Biophys

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Investigations into the configuration of the radiation induced strand breaks in the low dose range are presented. DNA sections containing the radiation induced strand breaks were separated from the undamaged sections in order to increase the concentration of lesions. The configuration of 3'terminals in damaged DNA from gamma-irradiated thymocytes was analysed studying the priming ability for the DNA polymerase I. The experiments show that soon after irradiation with a dose of 10 krad DNA strand breaks carry 3'OH end groups as well as damaged 3'terminals not susceptible to Dna polymerase I. The fraction of damaged terminals increases with dose. We conclude that also after low doses an exonucleolytic action is required in cells for removing damaged 3'terminals before repair DNA synthesis can take place.

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In vitro repair of radiation-induced strand breaks in DNA

Cited by 32 publications

References 16 publications

The DNA Exonucleases of Escherichia coli

The DNA Exonucleases of Escherichia coli

Exonuclease III and endonuclease IV remove 3' blocks from DNA synthesis primers in H2O2-damaged Escherichia coli.

End group characterization in DNA of thymocytes after low doses of ionizing radiation

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