In vitro release of silver ions and expression of osteogenic genes by MC3T3-E1 cell line cultured on nano-hydroxyapatite and silver/strontium-coated titanium plates

Lafzi, Ardeshir; Nejad, Azadeh Esmaeil; Rad, Maryam Rezai; Namdari, Mahshid; Sabetmoghaddam, Tohid

doi:10.1007/s10266-022-00747-z

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…In a similar study, Lee et al 29 found that there was no significant difference in cell proliferation between the control (Ti) and the HA-coated Ti surfaces via the LTHSC technique. Additionally, a study by Lafzi et al, 70 measured osteogenic gene expression in MC3T3-E1 cells on nano-HA-coated Ti substrates. Their results showed better expression on day 7 as compared to day 14, suggesting better gene expressions.…”

Section: Discussionmentioning

confidence: 99%

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

Swain,

Pradhan,

Bhuyan

et al. 2024

IJN

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There is an ongoing need for improved healing response and expedited osseointegration on the Ti implants in acetabular fracture sites. To achieve adequate bonding and mechanical stability between the implant surface and the acetabular fracture, a new coating technology must be developed to promote bone integration and prevent bacterial growth. Methods: A cylindrical Ti substrate mounted on a rotating specimen holder was used to implant Ca 2+ , P 2+ , and Sr 2+ ions at energies of 100 KeV, 75 KeV and 180 KeV, respectively, using a low-energy accelerator to synthesize strontium-substituted hydroxyapatite at varying conditions. Ag 2+ ions of energy 100 KeV were subsequently implanted on the as-formed surface at the near-surface region to provide anti-bacterial properties to the as-formed specimen. Results:The properties of the as-formed ion-implanted specimen were compared with the SrHA-Ag synthesized specimens by cathodic deposition and low-temperature high-speed collision technique. The adhesion strength of the ion-implanted specimen was 43 ± 2.3 MPa, which is well above the ASTM standard for Ca-P coating on Ti. Live/dead cell analysis showed higher osteoblast activity on the ion-implanted specimen than the other two. Ag in the SrHA implanted Ti by ion implantation process showed superior antibacterial activity. Discussion: In the ion implantation technique, nano-topography patterned surfaces are not concealed after implantation, and their efficacy in interacting with the osteoblasts is retained. Although all three studies examined the antibacterial effects of Ag 2+ ions and the ability to promote bone tissue formation by MC3T3-E1 cells on SrHA-Ag/Ti surfaces, ion implantation techniques demonstrated superior ability. The synthesized specimen can be used as an effective implant in acetabular fracture sites based on their mechanical and biological properties.

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Section: Discussionmentioning

confidence: 99%

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

Swain,

Pradhan,

Bhuyan

et al. 2024

IJN

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“…Strontium has gained attention because it is an element that supports cell proliferation without having toxic effects, and perhaps its most important aspect relies on the fact that it supports the formation of new bone tissue by slowing down osteoclast activity [30]. This important feature makes it a desirable doping element for HAp in the case of implantable medical devices for patients with osteoporosis or osteopenia [31][32][33]. Nowadays, new solutions and approaches for inducing a local antibacterial and/or bactericidal effect are preferred in order to prevent implant-associated infections, rather than the administration of oral or systemic antibiotics.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Ag- and Sr-Doped Hydroxyapatite Coatings for Medical Applications

et al. 2023

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Osseointegration plays the most important role in the success of an implant. One of the applications of hydroxyapatite (HAp) is as a coating for metallic implants due to its bioactive nature, which improves osteoconduction. The purpose of this research was to assess the in vitro behavior of HAp undoped and doped with Ag and/or Sr obtained by galvanostatic pulsed electrochemical deposition. The coatings were investigated in terms of chemical bonds, contact angle and surface free energy, electrochemical behavior, in vitro biomineralization in acellular media (SBF and PBS), and biocompatibility with preosteoblasts cells (MC3T3-E1 cell line). The obtained results highlighted the beneficial impact of Ag and/or Sr on the HAp. The FTIR spectra confirmed the presence of hydroxyapatite within all coatings, while in terms of wettability, the contact angle and surface free energy investigations showed that all surfaces were hydrophilic. The in vitro behavior of MC3T3-E1 indicated that the presence of Sr in the HAp coatings as a unique doping agent or in combination with Ag elicited improved cytocompatibility in terms of cell proliferation and osteogenic differentiation. Therefore, the composite HAp-based coatings showed promising potential for bone regeneration applications.

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Engineered functional doped hydroxyapatite coating on titanium implants for osseointegration

Li,

Zhang,

Liao

et al. 2023

Journal of Materials Research and Technology

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In vitro release of silver ions and expression of osteogenic genes by MC3T3-E1 cell line cultured on nano-hydroxyapatite and silver/strontium-coated titanium plates

Cited by 4 publications

References 38 publications

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

On the Ion Implantation Synthesis of Ag-Embedded Over Sr-Substituted Hydroxyapatite on a Nano-Topography Patterned Ti for Application in Acetabular Fracture Sites

In Vitro Evaluation of Ag- and Sr-Doped Hydroxyapatite Coatings for Medical Applications

Engineered functional doped hydroxyapatite coating on titanium implants for osseointegration

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