In vitro regeneration, somatic hybridization and genetic transformation studies: an appraisal on biotechnological interventions in grasses

Giri, Charu Chandra; Praveena, M.

doi:10.1007/s11240-014-0653-7

Cited by 22 publications

(8 citation statements)

References 153 publications

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“…Genetic engineering is a promising technology, which allows for the improvement of the nutritional quality of staple crops by transferring desirable, heritable traits between unrelated plant species (De Steur, Demont, Gellynck, & Stein, 2017). It can be used to overcome the limitation of narrow genetic variation or the presence of hybridization barriers (Giri & Praveena, 2015). For instance, transgenic maize plants with reduced levels of phytate, and transgenic rice with increased numbers of aleurone cell layers, have been genetically engineered (J.…”

Section: 12) Genetic Biofortificationmentioning

confidence: 99%

New Insights to Zinc Biofortification of Wheat: Opportunities to Fine-tune Zinc Uptake, Remobilization and Grain Loading

Kamaral¹,

Neate²,

Gunasinghe³

et al. 2020

Preprint

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Wheat contains low grain zinc (Zn) due to its genetics and the physiochemical properties of the soil in which it is grown. Consequently, where wheat forms a major part of the human diet, bioavailable Zn is below dietary requirements. Understanding the regulation of genes responsible for cellular Zn-transport, particularly those responsible for the control of the biosynthesis pathway of nicotianamine, provides an opportunity to increase Zn loading into the grain. Decreasing the levels of phytic acid, an inhibitor of Zn absorption in humans, provides another opportunity to increase the bioavailability of grain Zn. Synchrotron X-ray fluorescence microscopy clearly demonstrated that the crease region of the wheat grain is a major bottleneck to Zn loading in the endosperm. Higher expression of Zn transporter families, particularly metal tolerance proteins and yellow stripe like transporter families in the aleurone layer are also likely to play a major role in determining grain Zn content. Finally, anatomical barriers in the vascular region at the base of the wheat grain are a major limitation to Zn loading. Modification of any of these traits through traditional plant breeding or gene editing provides an opportunity to increase the Zn concentration in wheat grain.

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Section: 12) Genetic Biofortificationmentioning

confidence: 99%

New Insights to Zinc Biofortification of Wheat: Opportunities to Fine-tune Zinc Uptake, Remobilization and Grain Loading

Kamaral¹,

Neate²,

Gunasinghe³

et al. 2020

Preprint

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“…Protocols to transform (embryogenic) calli using Agrobacterium, particle bombardment, and electroporation have been published and successfully used in perennial ryegrass (reviewed in [103]), although problematically low regeneration efficiencies are often reported. Additionally, it is challenging to perform the selfings needed in order to fix a transgene for further evaluation in this SI species.…”

Section: Transformation and Mutationmentioning

confidence: 99%

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

2016

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Abstract:The importance of haploid and doubled haploid (DH) techniques for basic and applied research, as well as to improve the speed of genetic gain when applied in breeding programs, cannot be overstated. They have become routine tools in several major crop species, such as maize (Zea mays L.), wheat (Triticum aestivum L.), and barley (Hordeum vulgare L.). DH techniques in perennial ryegrass (Lolium perenne L.), an important forage species, have advanced to a sufficiently successful and promising stage to merit an exploration of what their further developments may bring. The exploitation of both in vitro and in vivo haploid and DH methods to (1) purge deleterious alleles from germplasm intended for breeding; (2) develop mapping populations for genetic and genomic studies; (3) simplify haplotype mapping; (4) fix transgenes and mutations for functional gene validation and molecular breeding; and (5) hybrid cultivar development are discussed. Even with the comparatively modest budgets of those active in forage crop improvement, haploid and DH techniques can be developed into powerful tools to achieve the acceleration of the speed of genetic gain needed to meet future agricultural demands.

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“…There is increasing demand for turfgrass species with improved drought and salt tolerance due to limited water resources and increasing salinity soil worldwide and irrigation using saline water ( Pessarakli, 2018 ). Transgenics has provided an effective tool for turfgrass improvement ( Guo et al, 2003 ; Giri and Praveena, 2015 ; Luo et al, 2017 ). For example, broad abiotic stress tolerance is enhanced by overexpressing rice SUMO E3 ligase (OsSIZ1) or a cyanobacterial flavodoxin in transgenic creeping bentgrass ( Agrostis palustris L.) ( Li Z. et al, 2013 ; Li et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a NF-YC Gene Results in Enhanced Drought and Salt Tolerance in Transgenic Seashore Paspalum

Shi

Guo

2018

Front. Plant Sci.

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Seashore paspalum (Paspalum vaginatum O. Swartz) is an important warm-season turfgrass species. In this study we generated transgenic seashore paspalum overexpressing CdtNF-YC1, a nuclear factor Y transcription factor from hybrid bermudagrass (Cynodon dactylon × Cynodon transvaalensis). DNA blot hybridization and qRT-PCR analysis showed that CdtNF-YC1 was integrated into the genomes of transgenic seashore paspalum plants and expressed. Reduced relative water content (RWC) and survival rate and increased ion leakage were observed in both wild type (WT) and transgenic plants after drought stress, while transgenic plants had higher levels of RWC and survival rate and lower ion leakage than the WT. Maximal photochemical efficiency of photosystem II (Fv/Fm), chlorophyll concentration and survival rate were decreased after salt stress, while higher levels were maintained in transgenic plants than in WT. In addition, an increased Na+ content and decreased or unaltered K+ in leaves and roots were observed after salt treatment, while lower level of Na+ and higher levels of K+ and K+/ Na+ ratio were maintained in transgenic plants than in WT. The results indicated that overexpressing CdtNF-YC1 resulted in enhanced drought and salt tolerance in transgenic plants. Transcript levels of stress responsive genes including PvLEA3, PvP5CS1, PvABI2, and PvDREB1B were induced in response to drought and salt stress, and higher levels were observed in transgenic seashore paspalum than in WT. The results suggest that the enhanced drought and salt tolerance in transgenic seashore paspalum is associated with induction of a series of stress responsive genes as a result of overexpression of CdtNF-YC1.

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In vitro regeneration, somatic hybridization and genetic transformation studies: an appraisal on biotechnological interventions in grasses

Cited by 22 publications

References 153 publications

New Insights to Zinc Biofortification of Wheat: Opportunities to Fine-tune Zinc Uptake, Remobilization and Grain Loading

New Insights to Zinc Biofortification of Wheat: Opportunities to Fine-tune Zinc Uptake, Remobilization and Grain Loading

Haploid and Doubled Haploid Techniques in Perennial Ryegrass (Lolium perenne L.) to Advance Research and Breeding

Overexpression of a NF-YC Gene Results in Enhanced Drought and Salt Tolerance in Transgenic Seashore Paspalum

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