In vitro regeneration of plantlets from unpollinated ovary culture in sweet orange (Citrus sinensis L. Osbeck)

Abstract

Callogenesis and organogenesis of ovary of sweet orange (Citrus sinensis) cv. Blood red was carried on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of N 6 benzyl adenine (BA), 1-napthaleneacetic acid (NAA) and 2,4-D. 1 mg/l BA + 0.5 mg/l NAA on MS medium was the most effective in callus induction and proliferation. Maximum number of shoots (11) was recorded on the medium with 2 mg/l NAA + 3 mg/l BA. The best medium for root induction was MS together with 2.5 mg/l indole-3-acetic acid (IAA) + 2 mg/l indole-3-butyric acid (IBA), where maximum (16) plantlets were rooted. The regenerated plantlets were successfully acclimatized in jiffy pots containing sterilized soil mixture of sand, silt and clay in 1:1:1 ratio to study their response to in vivo conditions.

Callus Induction and Plant Regeneration in Punica Granatum L. ʻnanaʼ From Leaf Explants

“…In present study, we found that rooting on WPM medium containing low level of IBA was the best treatment for rooting of dwarf pomegranates. IBA was reported to have favored root induction in pomegranate (Naik, et al, 1998), sweet orange (Khalil, et al, 2011) and in Citrus limonia Osbeck (Jajoo, 2010, Omura, et al, 1987 reported optimum rooting of dwarf pomegranate was obtained in MS/2 medium supplemented with .01 to 0.1 µM NAA.This method of propagation from leaf explants and further shoot proliferation and plantlet formation in 4-month duration could be alternatively used instead of conventional methods of propagation of dwarf pomegranate.…”