In Vitro Regeneration of ICP 8863 Pigeon Pea (Cajanus cajan (L.) Millsp.) Variety using Leaf Petiole and Cotyledonary Node Explants and Assessment of their Genetic Stability by RAPD Analysis

Nalluri, Nirmala; Karri, Vasavi Rama

doi:10.17485/ijst/2019/v12i9/140847

Cited by 3 publications

(2 citation statements)

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“…In Treatment T 1 , where 2 mg/l BAP was applied, a noteworthy increase in shoot production was observed, averaging 7.3±0.88 shoots per replicate (Table 3). These findings align with earlier reports by Geetha et al (1998) [8] and Nalluri and Karri (2019) [9] . Nevertheless, the challenges associated with this treatment included the formation of multiple shoots and the yellowing/falling of leaves (Fig.…”

Section: Organogenesis 311 Effect Of Growth Regulator Concentrations ...supporting

confidence: 93%

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Exploring in vitro shoot regeneration studies in pigeon pea (Cajanus cajan) via organogenesis and somatic embryogenesis

Wankhade,

PK,

et al. 2024

Int. J. Adv. Biochem. Res.

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In this study, we explored the In vitro shoot regeneration potential of pigeon pea (Cajanus cajan) cv. BSMR 853 through two distinct pathways: organogenesis and somatic embryogenesis. The use of different plant growth regulators and culture conditions was investigated to optimize shoot regeneration efficiency. For organogenesis, embryos from 5-day-old germinated seeds were employed, and we observed that the highest shoot bud regeneration occurred on media supplemented with 2 mg/L 6benzylaminopurine (BAP). Subsequent elongation of shoots was achieved by reducing the BAP concentration. In contrast, somatic embryogenesis was initiated from embryo of 5-day-old germinated seeds. Here thidiazuron (TDZ) was used to instead of BAP as a growth regulator. The development of globular embryos was rapid, and the embryos were prolific with TDZ. These globular embryos were then matured using MS media supplemented with abscisic acid (ABA) and subsequently germinated on half-strength MS media supplemented with lower concentrations of BAP. Comparative analysis of the two regeneration pathways in pigeon pea cv. BSMR 853 revealed that despite somatic embryogenesis producing a greater number of embryos, organogenesis led to a greater success rate in establishing shoots. This study provides valuable insights into the regeneration processes of pigeon pea, offering a basis for further research and applications in crop improvement and biotechnology.

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Section: Organogenesis 311 Effect Of Growth Regulator Concentrations ...supporting

confidence: 93%

“…l). Similar results were obtained by Nalluri and Karri (2019) [9] for the ICPL 87 variety of pigeon pea using leaf petiole explants. However, the shoots produced in this treatment were slender.…”

Section: Organogenesis 311 Effect Of Growth Regulator Concentrations ...supporting

confidence: 89%

Exploring in vitro shoot regeneration studies in pigeon pea (Cajanus cajan) via organogenesis and somatic embryogenesis

Wankhade,

PK,

et al. 2024

Int. J. Adv. Biochem. Res.

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Over-expression of Trigonella foenum-graecum defensin (Tfgd2) and Raphanus sativus antifungal protein (RsAFP2) in transgenic pigeonpea confers resistance to the Helicoverpa armigera

Nalluri

Karri

2022

Plant Cell Tiss Organ Cult

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Pigeonpea is an important food legume crop cultivated in tropical and sub-tropical regions around the world wherein the Indian subcontinent accounting for over 90 % of global production. It is a rich source of protein and is an important component of a well-balanced diet for the majority of Indians. Its productivity is affected by many insect pests among which Helicoverpa. armigera is the most signi cant pest in producing severe yield loss. Non-availability of resistant genes in germplasm and constraints with traditional breeding induce the application of a genetic engineering approach to generate insect resistance in pigeonpea. Expression of plant defensins in various crops provided enhanced resistance towards a variety of pests and pathogens. In the current study, two defensins Trigonella foenum-graecum defensin 2 (Tfgd2) and Raphanus sativus antifungal protein 2 (RsAFP2) integrated by a linker peptide was transferred into pigeonpea as a fusion gene by Agrobacterium mediated transformation. Putative transgenic lines were con rmed through PCR and the promising lines were identi ed in the following generations based upon integration, expression and bioe ciency of the fusion gene.Leaf bioassay conducted against H. armigera larvae showed increased levels of insect resistance compared to the control, where six T2 plants were identi ed as superior lines showing less than 25 % of leaf damage.Our ndings illustrates that Tfgd2-RsAFP2 fusion protein is e cient in imparting protection against the insect pest and the transgenic lines developed in this study could be used for further pigeonpea improvement projects. Key MessageOver-expression of Tfgd2-RsAFP2 fusion gene conferred enhanced insect resistance against Helicoverpa armigera in transgenic pigeonpea plants.

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Genotype-Dependent in Vitro Regeneration Assessment from Decapitated Embryonal Axis and Stem-Node Explants among Selected Pigeonpea Varieties

Kashyap¹,

Sarangi²,

Yadav³

et al. 2020

Biosci., Biotech. Res. Asia

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The inherent regeneration ability among selected varieties of pigeonpea using decapitated embryonal axis and stem-node explants in the presence of different growth regulators were assessed for multiple shoot bud induction. Among three different hormones namely BAP, Kinetin and TDZ tested for in vitro regeneration at different concentration for decapitated embryonal axis explants, BAP was found to be comparatively better as evident from number of buds per explants. IPA-242 variety was found to be the best for direct organogenesis resulting in the formation of 10 buds when subjected to MS Medium supplemented with 2.0 mgL-1 of BAP. Under treatment with different concentration of TDZ, Pusa-9 revealed 10 shoot buds with 0.15 mgL-1 of TDZ. The overall response of these varieties at different concentration of kinetin was very poor. With stem-node explants under variable concentration of BAP, IPA-3088 performed best showing 17 buds per explants. Similarly Pusa-9 and IPA 3088 revealed maximum regeneration ability forming 10 and 8 buds under TDZ and kinetin respectively. NAA was found to be effective growth regulator for rooting of shoots regenerated both from decapitated embryonal axis and stem-node explants.

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In Vitro Regeneration of ICP 8863 Pigeon Pea (Cajanus cajan (L.) Millsp.) Variety using Leaf Petiole and Cotyledonary Node Explants and Assessment of their Genetic Stability by RAPD Analysis

Cited by 3 publications

References 1 publication

Exploring in vitro shoot regeneration studies in pigeon pea (Cajanus cajan) via organogenesis and somatic embryogenesis

Exploring in vitro shoot regeneration studies in pigeon pea (Cajanus cajan) via organogenesis and somatic embryogenesis

Over-expression of Trigonella foenum-graecum defensin (Tfgd2) and Raphanus sativus antifungal protein (RsAFP2) in transgenic pigeonpea confers resistance to the Helicoverpa armigera

Genotype-Dependent in Vitro Regeneration Assessment from Decapitated Embryonal Axis and Stem-Node Explants among Selected Pigeonpea Varieties

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