In vitro regeneration of eight cultivars of Brassica oleracea var. capitata

Gerszberg, Aneta; Hnatuszko-Konka, Katarzyna; Kowalczyk, Tomasz

doi:10.1007/s11627-014-9648-7

Cited by 44 publications

(54 citation statements)

References 22 publications

(37 reference statements)

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“…The addition of NAA progressively increased the effectiveness of root formation, but seemed to cause alteration of morphology of both roots and whole shoots. Similar influence was reported by Gerszberg et al (2015) in B. oleracea var. capitata .…”

Section: Discussionsupporting

confidence: 90%

“…As it can be seen from the literature data, the prerequisite for genetic engineering—a plant regeneration system for in vitro propagation—has been established for many Brassica vegetables, e.g. reports by Gerszberg et al 2015; Kumar and Srivastava 2016; Munshi et al 2007; Pavlović et al 2010; Ravanfar et al 2014; Shahid et al 2010. Genetic transformation of the cole crops was achieved by direct DNA uptake and by the Rhizobium -mediated technique (Bhalla and Singh 2008; Chakrabarty et al 2002; Nugent et al 2006; Radchuk et al 2002).…”

Section: Introductionmentioning

confidence: 99%

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High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

et al. 2018

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Brassica oleracea var. botrytis, a very popular crop grown for its edible inflorescence, is bred only as a mutated annual cultivar and does not naturally occur in environment. Since cauliflower is still described as the most troublesome of all the B. oleracea vegetables regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic modifications. Here, we present a successful protocol for genetic transformation of cauliflower employing the process of agroinfection. The primary analysis of in vitro response of five cultivars allowed us to have chosen Pionier as the most promising cultivar; in consequence the Pionier was transformed via Rhizobium-mediated techniques in order to evaluate both, R. radiobacter (EHA 105, LBA 4404) and R. rhizogenes (ATCC 18534, A4) species. However, the latter system turned out to be more effective and, the A4 strain, in particular (72% transformation efficiency, 55% confirmed by GUS assay). That shows a promising technical advance especially when compared to the results of previous literature reports (e.g. 8.7% reported efficiency using R. rhizogenes). The transgenic cauliflower was obtained from hairy roots via organogenic callus induction. The potential transformants were analysed at the genomic and proteomic levels and their transgenic character was fully confirmed.Electronic supplementary materialThe online version of this article (10.1186/s13568-018-0656-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

et al. 2018

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“…These results are in agreement with data of Zhang and Bhalla (2004) on B. napus and with our previous study on B. oleracea var. capitata (Gerszberg et al, 2015a). Another study pointed out that the part of cotyledon used affects the morphogenesis process (Zhang et al, 2012).…”

Section: Culture Conditionsmentioning

confidence: 99%

“…The frequency of shoot regeneration was evaluated for cotyledons and hypocotyls that had been cultured for 30 days, as described in our previous work (Gerszberg et al, 2015a).…”

Section: Introductionmentioning

confidence: 99%

Efficient In Vitro Callus Induction and Plant Regeneration Protocol for Different Polish Tomato Cultivars

Gerszberg¹,

Hnatuszko-Konka²,

Kowalczyk³

et al. 2016

Not Bot Horti Agrobo

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The major goal of this research was to establish a stable regeneration system for tomato cultivars in order to lay the foundations for the future genetic transformation of the tomato. The regeneration ability of two kinds of explants (cotyledons and hypocotyl segments) was compared for three Polish cultivars of the tomato (Solanum lycopersicum). Explants were cultured on 10 different regeneration media (basal mediums MS or B5, and with a combination of 6-benzylaminopurine (BAP) and indole-3-acetic acid (IAA). It was found that the ability to regenerate was substantially dependent on the cultivars, as well as on the kind of explant. The best explants for inducing shoot regeneration were cotyledons, followed by hypocotyls. It was noticed that the best formulation of the medium for this regeneration from the two types of explants used in this study, is MS with 2 mg/L BA and 0.1 mg/L IAA. Tomato shoots were transferred to a ½ MS medium and ½ MS complemented with 0.1 mg/L IAA for rooting and all of them responded positively to the rooting medium.

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“…The regeneration of these cultivars, including that of Aari canola, could be further improved upon by optimizing the plant regeneration conditions. It has been observed that varying the media components affects the shoot formation efficiencies significantly [33,[35][36][37]42,43,[45][46][47]. The fact that huge variations have been observed among different explants, a generalized recommendation cannot be made.…”

Section: Discussionmentioning

confidence: 99%

Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica

Farooq

Nawaz

Mukhtar

et al. 2019

Plants

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In vitro regeneration is a pre-requisite for developing transgenic plants through tissue culture-based genetic engineering approaches. Huge variations among different genotypes of the genus Brassica necessitate the identification of a set of regeneration conditions for a genotype, which can be reliably used in transformation experiments. In this study, we evaluated the morphogenesis potential of four commercial cultivars (Faisal canola, Punjab canola, Aari canola, Nifa Gold) and one model, Westar, from four different explants namely cotyledons, hypocotyls, petioles and roots on three different Brassica regeneration protocols, BRP-I, -II and -III. The regeneration efficiency was observed in the range of 6-73%, 4-79.3%, 0-50.6%, and 0-42.6% from cotyledons, petioles, hypocotyls and roots, respectively, whereas, the regeneration response in terms of average shoots per explant was found to be 0.76-10.9, 0.2-3.2, 0-3.4 and 0-2.7 from these explants. Of the commercial varieties tested, almost all varieties showed poorer regeneration than Westar except Aari canola. In comparison to Westar, its regeneration frequency from cotyledons was up to 7.5-fold higher on BRP-I, while it produced up to 21.9-fold more shoots per explant. Our data show that the explant has strong influence on the regeneration response, ranging from 24% to 92%. While the growth of commercial cultivars was least affected by the regeneration conditions provided, the effect on Westar was twice that of the commercial cultivars. After determining the optimal explant type and regeneration conditions, we also determined the minimum kanamycin concentration levels required to selectively inhibit the growth of untransformed cells for these cultivars. Regenerated shoots of Aari canola could be successfully grown to maturity within 16-18 weeks, with no altered phenotype noted and normal seed yields obtained. Therefore, the commercial variety, Aari canola, could be a good candidate for future genetic transformation studies.

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In vitro regeneration of eight cultivars of Brassica oleracea var. capitata

Cited by 44 publications

References 22 publications

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes

Efficient In Vitro Callus Induction and Plant Regeneration Protocol for Different Polish Tomato Cultivars

Investigating the In Vitro Regeneration Potential of Commercial Cultivars of Brassica

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