Abstract:This study was conducted to establish a protocol for in vitro flowering of Centaurea cyanus and cell cultures of Centaurea montana. In four weeks, 50 to 60 adventitious shoots developed on leaf explants cultured in MS medium supplemented with 2.0 mg/L benzylaminopurine (BAP) and 0.1 mg/L indole-3acetic acid (IAA). In vitro flowering and seed set occurred in C. cyanus when the shoots were incubated on MS basal medium supplemented with B5 vitamins, 100 mg/L myo-inositol and 30 g/L sucrose for 4 weeks under 16 h … Show more
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