In vitro regeneration and Agrobacterium tumefaciens-mediated genetic transformation of Parkia timoriana (DC.) Merr.: a multipurpose tree legume

Thangjam, Robert; Sahoo, Lingaraj

doi:10.1007/s11738-011-0917-3

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…Low rooting ability is a limiting factor in the micropropagation of woody plant species (Dewir et al, 2016;Nemeth, 1986). Our results confirm previous findings on in vitro rooting of Parkia timoriana (Thangjam and Sahoo, 2012) and Syzyigium cordatum (Dewir et al, 2011) in which rooting was dependent on medium strength, and the type and concentration of auxins. In an attempt to increase the rate of rooting in C. erectus, different sucrose concentrations and application of activated charcoal were tested; none of the tested treatments increased the rate of rooting (data not presented).…”

Section: Resultssupporting

confidence: 91%

Micropropagation of Buttonwood Tree (Conocarpus erectus) through Axillary Shoot Proliferation

Dewir

Aldubai

El-Hendawy

et al. 2018

horts

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A method for micropropagation of Conocarpus erectus through axillary shoot proliferation is presented. Shoot tips were excised from adult donor tree and cultured for 4 weeks on Murashige and Skoog’s (MS) medium supplemented with 3 mg·L−1 gibberellic acid (GA3) to induce sprouting of shoots and formation of axillary shoots. Conocarpus erectus shoots were cultured for 6 weeks on MS medium supplemented with different concentrations and combinations of plant growth regulators (PGRs) and proliferation of the shoots was monitored. The type and concentration of cytokinins applied had a significant influence on shoot proliferation responses. Supplementation with 6-benzylaminopurine (BAP) increased the rate of shoot proliferation compared with other cytokinins. The use of BAP in combination with auxins such as indole-3-butyric acid (IBA) and naphthalene acetic acid (NAA) resulted in an increased number of shoots per explant compared with treatment with BAP alone. A combination of 2 mg·L−1 BAP and 0.5 mg·L−1 IBA produced the highest number of axillary shoots (7.8 shoots/explant). The best rooting medium was full-strength MS medium supplemented with 1 mg·L−1 IBA; this treatment yielded 80% rooting with an average of 3.5 roots per plantlet. All regenerated plantlets were successfully acclimatized to greenhouse conditions.

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Section: Resultssupporting

confidence: 91%

Micropropagation of Buttonwood Tree (Conocarpus erectus) through Axillary Shoot Proliferation

Dewir

Aldubai

El-Hendawy

et al. 2018

horts

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“…Establishment of in vitro propagation protocols is a prerequisite for genetic manipulation studies because in traditional breeding techniques—such as backcrossing and selfing—it is difficult to fix desirable alleles in a particular genetic background [ 13 ]. Successful transfer of genes of interest through genetic transformation technique has been well documented in many tree species [ 329 , 330 , 331 ]. Gorpenchenko et al [ 332 ] established a successful genetic transformation process in Panax ginseng through callus-based shoot organogenesis.…”

Section: Molecular Studies Of P Marsupiummentioning

confidence: 99%

“…Interestingly, Jube and Borthakur [ 333 ] proposed that the rate of transformation in woody species is meager because excised explants produce several harmful phenolic compounds. On a contrary note, there are many reports published on A. tumefaciens -mediated transformation in leguminous woody trees [ 330 , 331 ].…”

Section: Molecular Studies Of P Marsupiummentioning

confidence: 99%

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Ahmad

Anis

et al. 2022

Plants

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Trees are vital resources for economic, environmental, and industrial growth, supporting human life directly or indirectly through a wide variety of therapeutic compounds, commodities, and ecological services. Pterocarpus marsupium Roxb. (Fabaceae) is one of the most valuable multipurpose forest trees in India and Sri Lanka, as it is cultivated for quality wood as well as pharmaceutically bioactive compounds, especially from the stem bark and heartwood. However, propagation of the tree in natural conditions is difficult due to the low percentage of seed germination coupled with overexploitation of this species for its excellent multipurpose properties. This overexploitation has ultimately led to the inclusion of P. marsupium on the list of endangered plant species. However, recent developments in plant biotechnology may offer a solution to the overuse of such valuable species if such advances are accompanied by technology transfer in the developing world. Specifically, techniques in micropropagation, genetic manipulation, DNA barcoding, drug extraction, delivery, and targeting as well as standardization, are of substantial concern. To date, there are no comprehensive and detailed reviews of P. marsupium in terms of biotechnological research developments, specifically pharmacognosy, pharmacology, tissue culture, authentication of genuine species, and basic gene transfer studies. Thus, the present review attempts to present a comprehensive overview of the biotechnological studies centered on this species and some of the recent novel approaches for its genetic improvement.

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“…Its pods are consumed as vegetable and salad while branches and wood are used as firewood or as timber. The first report of successful in vitro regeneration and establishment in the field for transgenic P. timoriana came from Thangjam and Sahoo (2012). They used cotyledonary node explants for transformation experiments with A. tumefaciens strain EHA105 harbouring a binary vector pCAMBIA2301 which contains b-glucuronidase (GUS) with an intron in the coding region and neomycin phosphotransferase (nptII) gene, both driven by CaMV35S promoter.…”

Section: Miscellaneous Grain and Medicinal Legume Speciesmentioning

confidence: 99%

Gene Transfer in Legumes

Atif¹,

Patat-Ochatt²,

Švábová

et al. 2012

Progress in Botany

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In vitro regeneration and Agrobacterium tumefaciens-mediated genetic transformation of Parkia timoriana (DC.) Merr.: a multipurpose tree legume

Cited by 13 publications

References 20 publications

Micropropagation of Buttonwood Tree (Conocarpus erectus) through Axillary Shoot Proliferation

Micropropagation of Buttonwood Tree (Conocarpus erectus) through Axillary Shoot Proliferation

Biotechnological Advances in Pharmacognosy and In Vitro Manipulation of Pterocarpus marsupium Roxb.

Gene Transfer in Legumes

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