In vitro reconstruction of human dermal equivalent enriched with endothelial cells

Tonello, C; Zavan, Barbara; Cortivo, Roberta; Brun, Paola; Panfilo, Susi; Abatangelo, Giovanni

doi:10.1016/s0142-9612(02)00450-7

Cited by 67 publications

(41 citation statements)

References 33 publications

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“…Finally, the dimensions of the fibroblast gel region, in combination with cell seeding density, determine the rate of secretion of growth factors and cytokines. We chose dimensions that enabled us to screen for the optimal seeding ratio between HUVECs and NHLFs within a range of 1:2-2:1 that is physiologically relevant and reported in other in vitro studies 16,17 (Results discussed below).…”

Section: Device Design and Fabricationmentioning

confidence: 99%

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Whisler

Chen

Kamm

2014

Tissue Engineering Part C: Methods

204

233

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The mechanical and biochemical microenvironment influences the morphological characteristics of microvascular networks (MVNs) formed by endothelial cells (ECs) undergoing the process of vasculogenesis. The objective of this study was to quantify the role of individual factors in determining key network parameters in an effort to construct a set of design principles for engineering vascular networks with prescribed morphologies. To achieve this goal, we developed a multiculture microfluidic platform enabling precise control over paracrine signaling, cell-seeding densities, and hydrogel mechanical properties. Human umbilical vein endothelial cells (HUVECs) were seeded in fibrin gels and cultured alongside human lung fibroblasts (HLFs). The engineered vessels formed in our device contained patent, perfusable lumens. Communication between the two cell types was found to be critical in avoiding network regression and maintaining stable morphology beyond 4 days. The number of branches, average branch length, percent vascularized area, and average vessel diameter were found to depend uniquely on several input parameters. Importantly, multiple inputs were found to control any given output network parameter. For example, the vessel diameter can be decreased either by applying angiogenic growth factors-vascular endothelial growth factor (VEGF) and sphingosine-1-phsophate (S1P)-or by increasing the fibrinogen concentration in the hydrogel. These findings introduce control into the design of MVNs with specified morphological properties for tissue-specific engineering applications.

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Section: Device Design and Fabricationmentioning

confidence: 99%

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Whisler

Chen

Kamm

2014

Tissue Engineering Part C: Methods

204

233

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“…In particular, HYAFF-11 ( a c o m p l e t e l y e s t e r i f i e d h y a l u r o n a n d e r i v a t i v e ) i s u s e d i n m a n y m e d i c a l applications for tissue repair, controlled drug release, nerve regeneration, wound dressing. It proved effective as a scaffold for skin and cartilage regeneration (Caravaggi et al, 2003;Grigolo et al, 2002;Tonello et al, 2003). It is available in several forms: films, gauzes, sponges, tubes and microsphere.…”

Section: Derivatized Ha Applicationsmentioning

confidence: 99%

Biotechnological Production and Application of Hyaluronan

Schiraldi¹,

Gatta²,

Rosa³

2010

Biopolymers

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“…The particles had a nano-structured porosity that was particularly suitable for absorbing bioactive molecules. HYAFF11 Ò , the benzyl ester of hyaluronic acid (Fidia advanced Biopolymer, Italy), is a biopolymer well known in tissue engineering applications such as in vitro reconstruction of skin, cartilage, and bone, and has been recently used for the in vivo regeneration of small arteries [12][13][14][15][16]. Microparticles, films or plugs prepared from hyaluronan esters have also been evaluated as a novel drug delivery system [17,18].…”

Section: Introductionmentioning

confidence: 99%

Hyaluronan based porous nano-particles enriched with growth factors for the treatment of ulcers: a placebo-controlled study

Zavan

Vindigni

Vezzù

et al. 2008

J Mater Sci: Mater Med

Self Cite

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The present study describes the production of hyaluronan based porous microparticles by a semi-continuous gas anti-solvent (GAS) precipitation process to be used as a growth factor delivery system for in vivo treatment of ulcers. Operative process conditions, such as pressure, nozzle diameter and HYAFF11 Ò solution concentrations, were adjusted to optimize particle production in terms of morphology and size. Scanning electron microscopy (SEM) and light scattering demonstrated that porous nano-structured particles with a size of 300 and 900 nm had a high specific surface suitable for absorption of growth factors from the aqueous environment within the polymeric matrix. Water acted as a plasticizer, enhancing growth factor absorption. Water contents within the HY-AFF11 Ò matrix were analyzed by differential scanning calorimetry (DSC). The absorption process was developed using fluorescence dyes and growth factors. Immunohistochemical analysis confirmed the high efficiency of absorption of growth factor and a mathematical model was generated to quantify and qualify the in vitro kinetics of growth factor release within the polymeric matrix. In vivo experiments were performed with the aim to optimize timed and focal release of PDGF to promote optimal tissue repair and regeneration of full-thickness wounds.

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In vitro reconstruction of human dermal equivalent enriched with endothelial cells

Cited by 67 publications

References 33 publications

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Control of Perfusable Microvascular Network Morphology Using a Multiculture Microfluidic System

Biotechnological Production and Application of Hyaluronan

Hyaluronan based porous nano-particles enriched with growth factors for the treatment of ulcers: a placebo-controlled study

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