In vitro reconstructed normal human epidermis expresses differentiation-related proteoglycans

Haftek, Marek; Bitoux, Marie-Aude Le; Callejon, Sylvie; Denis, Alain; Pernet, Ingrid; Amsellem, Carole; Bertino, Béatrice

doi:10.1016/j.jdermsci.2008.03.007

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Future studies are needed to fully understand functions of the 18 CD44 variants in cell survival, cell proliferation or resistance to apoptosis in human epidermis. Their functions should be influenced not only by the peptide sequences but also by the nature, position and quantity of glycans decorating their external portions [ 44 , 83 – 85 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

et al. 2016

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CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.

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Section: Discussionmentioning

confidence: 99%

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

et al. 2016

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“…Their functions should be influenced not only by the peptide sequences but also by the nature, position and quantity of glycans decorating their external portions [44,[83][84][85].…”

Section: Discussionmentioning

confidence: 99%

Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

Teye

Numata

Krol

et al. 2016

Journal of Dermatological Science

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Syndecans and CD44 in Normal Human Keratinocyte Cultures: Modulation with Medium Composition and All-trans Retinoic Acid

Le¹,

Callejon²,

Rodriguez³

et al. 2009

TODJ

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Abstract:We investigated chosen epidermal proteoglycans in different culture models to explore their expression by keratinocytes at various states of differentiation. Fully defined conditions of our epidermal reconstructions allowed for evaluation of the proteoglycans in "normal" or "ageing"-like epidermal tissues in vitro. The "ageing"-like epidermis was characterized by the viable layers' atrophy, low proliferation rate and premature keratinisation. Expression of all studied proteoglycan genes could be detected with quantitative real-time PCR but the syndecan-4 protein wasn't observed in the reconstructed epidermis using immunohistochemistry. Treatment with all-trans retinoic acid resulted in epithelial acanthosis, combined with up-regulation of proteoglycans in the "ageing"-like culture conditions. In "normal" reconstructed epidermis, retinoids increased the syndecan-4 gene expression but down-regulated that of syndecan-1. The observed complex changes are compatible with the presumed but still poorly understood roles of various proteoglycans in epidermal differentiation. Our defined culture models are suitable for screening of pharmacological agents modulating epidermal proteoglycan expression.

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In vitro reconstructed normal human epidermis expresses differentiation-related proteoglycans

Cited by 3 publications

References 6 publications

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

Syndecans and CD44 in Normal Human Keratinocyte Cultures: Modulation with Medium Composition and All-trans Retinoic Acid

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