In vitro Reconstitution of the Immune Response of Thymus-deprived Mice to Sheep Red Blood Cells

Munro, Alan; Hunter, Philippa

doi:10.1038/225277a0

Cited by 53 publications

(31 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It can also be seen that the factor nonselectively activated B lymphocytes from B 10.A(4R), B 10, and B 10.D2 mice and therefore can be considered nonspecific. Discussion A number of studies have demonstrated the ability of allogeneic lymphocytes to activate humoral antibody synthesis in vitro (9,11,14,15). Earlier workers found that the primary immune SRBC response of cells from either adult thymectomized, irradiated, bone-marrow protected mice (14) or neonatally thymectomized mice (7) could be restored by the addition of allogeneic spleen cells to the in vitro culture.…”

Section: Specificity Of An Allogeneic Effect Factormentioning

confidence: 99%

“…Discussion A number of studies have demonstrated the ability of allogeneic lymphocytes to activate humoral antibody synthesis in vitro (9,11,14,15). Earlier workers found that the primary immune SRBC response of cells from either adult thymectomized, irradiated, bone-marrow protected mice (14) or neonatally thymectomized mice (7) could be restored by the addition of allogeneic spleen cells to the in vitro culture. Lefkovits and coworkers extended these observations by showing, first, a 0-sensitive allogeneic T cell was required for eliciting the anti-SRBC response (16) and second, using limiting dilution analysis, specific alloantigen-reactive T cells mediated this response (17).…”

Section: Specificity Of An Allogeneic Effect Factormentioning

confidence: 99%

See 1 more Smart Citation

Continuously proliferating allospecific T cells. I. Specificity of cooperation with allogeneic B cells in the humoral antibody response to sheep erythrocytes.

Waterfield¹,

Dennert²,

Swain³

et al. 1979

The Journal of Experimental Medicine

View full text Add to dashboard Cite

Allospecific mouse T cells, positively selected in one-way mixed lymphocyte culture were maintained for 3 yr in tissue culture by sequential restimulation. Such proliferating T cells were tested for their ability to induce a positive allogeneic effect: activating B cells in an in vitro primary humoral response to sheep erythrocytes. It was found that such T lymphocytes could function as helper cells. Helper activity was shown to be specific in that the B cells activated had to share major histocompatibility complex (H-2) antigens with the strain used for selection of the cell line. Intra H-2 mapping showed that antigens coded in the IAk subregion played an important role in the induction of the positive allogeneic effect. Supernatant factors could substitute for the allogeneic T cells in activation of the in vitro humoral response. However, such supernates exhibited no strain specificity. Therefore, the specificity seen in the positive allogeneic effect is presumably a consequence of the alloantigenic recognition receptors intrinsic to the T cells, and not to any biologically restricting properties of the allogeneic effect factor itself.

show abstract

Section: Specificity Of An Allogeneic Effect Factormentioning

confidence: 99%

Section: Specificity Of An Allogeneic Effect Factormentioning

confidence: 99%

Continuously proliferating allospecific T cells. I. Specificity of cooperation with allogeneic B cells in the humoral antibody response to sheep erythrocytes.

Waterfield¹,

Dennert²,

Swain³

et al. 1979

The Journal of Experimental Medicine

View full text Add to dashboard Cite

show abstract

“…Thus, in certain conditions it would not be surprising to find sufficient shedding, either spontaneously or as a result of nonspecific stimulation in the culture medium, of a histocompatibility gene product normally present on the T-cell surface that might be capable of enhancing and/or reconstituting responses of other B cells. Furthermore, this hypothesis explains the capacity to observe "bystander" effects in mixed culture in vitro since in all such studies demonstrating this phenomenon, the responding bystander B-cell populations have been syngeneic or isologous with the effector T-cell population (3,15,(38)(39)(40).…”

Section: Discussionmentioning

confidence: 99%

“…These experiments are based on previous results of in vivo (36,37) and in vitro (38)(39)(40) experimentation in systems of T-B-cell cooperative interactions. One of the questions to which we have addressed our studies is whether an active moiety generated in vitro behaves in a virtually identical biological fashion as the phenomenon we termed the "allogeneic effect" operates in vivo (36,41).…”

Section: Discussionmentioning

confidence: 99%

Activation of T and B Lymphocytes in Vitro

Armerding

Katz

1974

The Journal of Experimental Medicine

211

View full text Add to dashboard Cite

The studies presented herein have focused on the biological and biochemical properties of a nonspecific mediator produced by populations of activated T lymphocytes during short-term in vitro reactions with foreign alloantigens. We have analyzed the activity of the unseparated and of chromatographically separated fractions of the supernatants from such cultures on the in vitro responses of mouse lymphocytes to soluble and macrophage-bound DNP-carrier conjugates and also to particulate heterologous erythrocytes. The results demonstrate that a highly active protein moiety, termed allogeneic effect factors (AEF), in the mol wt range of 30,000–40,000, is capable of acting directly on B lymphocytes, in the presence of antigen, probably to effect triggering and subsequent differentiation and proliferation to antibody-forming cells in vitro. The active molecule, although not manifesting specificity for antigen, does exhibit some strain-specific properties suggesting a possible relationship to cell surface antigens or other gene products coded in the major histocompatibility gene complex.

show abstract

“…Evidence obtained from such in vivo studies, was rather indirect and several studies had turned their attention to the use of an in vitro system to obtain more direct results on cell-cooperation. For this purpose, attempts were made to restore the in vitro response of immunologically impaired spleen cell cultures from mice, which had received either neonatal thymectomy or adult thymectomy and X-irradiation, by adding normal thymus cells or thymusderived cells [7,9,17].…”

mentioning

confidence: 99%

Cell‐Cooperation in Anti‐Sheep Red Blood Cell Antibody Response in Mouse Spleen Cell Cultures

Tadakuma

1972

Japanese Journal of Microbiology

View full text Add to dashboard Cite

Restoration of impaired antibody response to sheep red blood cells (SRBC) in spleen cell cultures from mice treated with heterologous antilymphocyte globulin (ALG) was studied by adding normal cells from various sources, to explore the problems of cell-cooperation in anti-SRBC antibody response and the target of ALG. When spleen cells from ALG-treated mice were separated into macrophage-rich and lymphoid cell-rich subpopulations, only the latter was found to be impaired in the ability for anti-SRBC antibody response. Addition of even a small number of normal allogeneic spleen cells sufficiently restored the impaired anti-SRBC antibody response of the spleen cells from ALG-treated mice. By use of allo-antisera, most hemolysin plaque-forming cells (PFC) generated in such cultures were proved to be derived from the cells of ALG-treated mice. Restoration was also achieved by adding thymus-derived cells, which were obtained from spleens of mice heavily irradiated and repopulated with syngeneic thymus cells, or lymphoid cells directly collected from thymuses. All results indicate that ALG selectively depletes the thymus-derived antigen reactive cells (ARC) in the spleen cell population, and that ARC supplied from normal spleen or thymus can interact with plaque-forming cell precursors (PFCP) that remain intact in the spleen cell population of ALG-treated mice. The results also suggest that a single ARC interacts with more than one PFCP and makes them develop into PFC.

show abstract

In vitro Reconstitution of the Immune Response of Thymus-deprived Mice to Sheep Red Blood Cells

Cited by 53 publications

References 12 publications

Continuously proliferating allospecific T cells. I. Specificity of cooperation with allogeneic B cells in the humoral antibody response to sheep erythrocytes.

Continuously proliferating allospecific T cells. I. Specificity of cooperation with allogeneic B cells in the humoral antibody response to sheep erythrocytes.

Activation of T and B Lymphocytes in Vitro

Cell‐Cooperation in Anti‐Sheep Red Blood Cell Antibody Response in Mouse Spleen Cell Cultures

Contact Info

Product

Resources

About