In Vitro Reactivity of Human Lymphocytes after Cryopreservation Using a Programmed Cooling Device

Bois, M. J. G. J.; Schellekens, P. T. A.; Wit, J. J. Fr. M. de; Eijsvoogel, V. P.

doi:10.1111/j.1365-3083.1976.tb03852.x

Cited by 43 publications

(8 citation statements)

References 5 publications

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“…1975) except that treatment with carbonyl iron was omitted. In the family studies, for reasons of convenience, all tests were performed on lymphocytes frozen-thawed according t o the method described by du Bois et al (1976). Cell viability was always better than 95% for fresh lymphocytes and 10% for frozenthawed lymphocytes.…”

Section: Cellsmentioning

confidence: 99%

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

et al. 1979

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Two hundred and eight pregnancy sera were tested for the presence of antibodies specific for lymphocyte sub‐populations by using the isolated B and T lymphocytes from the women's mating partners. This was done by the microlymphocytotoxicity and the indirect immuno‐fluorescence techniques. Five sera (2.5%) reacted exclusively with B lymphocytes and sixty‐three sera (30.2%) reacted with both B and T lymphocytes; none of the sera was specific for T cells. Several sera, reacting with both B and T lymphocytes, were absorbed with platelets and this procedure revealed nine additional antisera specific for B lymphocyte antigens. Specificity studies on a panel of forty‐eight HLA—ABCD typed individuals indicated that most antisera possibly defined new B‐cell antigens. Family studies established that the antigens defined by these antisera were coded for by genes in the Major Histocompatibility Complex.

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Section: Cellsmentioning

confidence: 99%

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

et al. 1979

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“…Sera were frozen and kept at -2 0 °C. Mononuclear cells were isolated from defibrinated blood by Ficoll-Isopaquc den sity gradient centrifugation and preserved in liquid nitrogen [4], The absolute numbers of total lymphocytes and T and B lympho cytes and monocytes were determined as described [5]. The percent ages of T lymphocyte subsets were analyzed by indirect immunoflu orescence using monoclonal antibodies of the OKT series (Ortho Pharmaceutical Laboratories, Beerse.…”

Section: Immunoreactivity In Vitromentioning

confidence: 99%

Clinical and Immunological Follow-Up of Patients with Severe Renal Disease in Wegener’s Granulomatosis

Wilmink

et al. 1985

Am J Nephrol

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Clinical and immunological data are reported of 12 patients suffering from Wegener’s granulomatosis and severe renal involvement. Although 9 patients recovered from their acute illness, at long-term follow-up a relapse occurred in 4 of these 9 patients. Therefore, lifelong follow-up in this group of patients seems to be mandatory. Extensive immunological investigations did not provide evidence for humoral mechanisms underlying the pathogenesis of this disease; T lymphocyte subsets in peripheral blood as well as functional reactivity of lymphocytes in vitro were also normal. However, none of the patients was able to mount a primary cellular immune response in vivo. On the other hand, kidney biopsy specimens obtained before the initiation of drug therapy revealed periglomerular and interstitial cellular infiltrations consisting predominantly of T lymphocytes with a ratio Leu 3a (OKT4)/Leu 2a (OKT8) of 5:1. This may indicate that a type IV (delayed-type) hypersensitivity reaction takes place in the kidney. These findings suggest that an abnormal cellular immunoreactivity plays a major role in the pathogenesis of Wegener’s granulomatosis.

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“…Cytotoxici ty was studied against solid tumor or adherent fibro blast target cells. Prolonged assay incubations were involved (18-40 h), and in some cases the thawed ef fector cells were preincubated overnight prior to the assays [DuBois et al, 1976;Naor and O'Toole, 1977;Bean et al, 1978]. When we performed 18-hour assays of ADCC and NK activity by cryopreserved PBM, recovery of cytotoxicity was similar to that observed in the 3-hour assays (data not shown).…”

Section: Discussionmentioning

confidence: 59%

Repetitive Testing and Storage of Human Effector Cells in Natural Killing and Antibody-Dependent Cell-Mediated Cytotoxicity

Hirsen¹,

Dubin²,

Malham³

1983

Int Arch Allergy Immunol

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Daily variation in natural killer (NK) and antibody-dependent cell-mediated cytotoxicity (ADCC) assays is problematic. When fresh peripheral blood mononuclear cells (PBM) from 4 donors were tested repeatedly, the ADCC and NK values tended to fall in the same rank order on each day tested, suggesting that repetitive assay of control donors could serve as an index for daily variation. Cryopreservation of PBM resulted in decreased and quite variable recovery of ADCC and NK. Cryopreservation, therefore, would not be an adequate control for daily variation. Storage of PBM at 4°C for 24 h resulted in better recovery of ADCC and NK activities than did cryopreservation.

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In Vitro Reactivity of Human Lymphocytes after Cryopreservation Using a Programmed Cooling Device

Cited by 43 publications

References 5 publications

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

Clinical and Immunological Follow-Up of Patients with Severe Renal Disease in Wegener’s Granulomatosis

Repetitive Testing and Storage of Human Effector Cells in Natural Killing and Antibody-Dependent Cell-Mediated Cytotoxicity

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In Vitro Reactivity of Human Lymphocytes after Cryopreservation Using a Programmed Cooling Device

Cited by 43 publications

References 5 publications

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

Detection of B‐Cell‐Specific Alloantibodies in Pregnancy Sera in the Lymphocytotoxicity and the Indirect Immunofluorescence Techniques

Clinical and Immunological Follow-Up of Patients with Severe Renal Disease in Wegener&rsquo;s Granulomatosis

Repetitive Testing and Storage of Human Effector Cells in Natural Killing and Antibody-Dependent Cell-Mediated Cytotoxicity

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Product

Resources

About

Clinical and Immunological Follow-Up of Patients with Severe Renal Disease in Wegener’s Granulomatosis