In vitro reactivation of latent equid herpesvirus-1 from CD5+/CD8+ leukocytes indirectly by IL-2 or chorionic gonadotrophin.

Smith, Daniel J.; Iqbal, Javed; Purewal, A.S.; Hamblin, Anne S.; Edington, N.

doi:10.1099/0022-1317-79-12-2997

Cited by 41 publications

(40 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Latent EHV-1 and EHV-4 infections in horse have been shown to reactivate from lymphoid tissues following the administration of corticosteroids (Edington et al, 1985;Edington et al, 1994a, b;Browning et al, 1988;Welch et al, 1992) and also by IL-2 or chorionic gonadotrophin stimulation of leukocytes (Smith et al, 1998). Borchers et al (1999) also have been able to detect latency associated transcripts of EHV-1 and EHV-4 by PCR from trigeminal ganglia of the latently infected horses.…”

Section: Discussionmentioning

confidence: 87%

See 1 more Smart Citation

Equid herpesvirus 1 is neurotropic in mice, but latency from which infectious virus can be reactivated does not occur

Iqbal

Edington

2002

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

Equid herpesvirus 1 (EHV-1) is the most common cause of virus-induced abortion in horses. After primary infection the virus becomes latent predominantly in the respiratory tract lymph nodes and the genome can also be detected in the peripheral nervous system. The role of mouse as a feasible model for the establishment of latency and reactivation of EHV-1 was investigated. Intracerebral and intranasal infections of 3-and 17-day-old mice were made and virus replication was confirmed by virus isolation and detected by indirect immunofluorescence (IIF) in brain. For reactivation studies, the mice were killed 8 weeks post infection and tissues were collected for cocultivation. In mice from both age groups, infectious virus was not detected by cocultivation. Following attempts to reactivate virus in vivo with corticosteroids, the viral antigen was detected at low levels by IIF and the expression of the gB gene by reverse transcription polymerase chain reaction (RT-PCR) in brain, trigeminal ganglia, olfactory lobe, lung and spleen. Virus was also detected by IIF following incubation of tissue explants in the growth medium containing pokeweed mitogen (PWM). These results show the limitations of the mouse model for investigating EHV-1 latency and highlights the issue of 'ineffective reactivation' of virus.

show abstract

Section: Discussionmentioning

confidence: 87%

“…Cocultivation was also carried out in the presence of IL-2 (20 U/ml) or pokeweed mitogen (PWM 100 µg/ml), which were known to reactivate EHV-1 from equine leukocytes (Smith et al, 1998).…”

Section: Latency and Reactivationmentioning

confidence: 99%

Equid herpesvirus 1 is neurotropic in mice, but latency from which infectious virus can be reactivated does not occur

Iqbal

Edington

2002

Acta Veterinaria Hungarica

View full text Add to dashboard Cite

show abstract

“…dexamethasone, cyclosporine A). Other studies report the presence of LAT mainly in lymphoid cells, with reactivation of EHV-1 by co-cultivation of PBMC with susceptible cells and only an occasional detection of LAT in the trigeminal ganglion [34,42].…”

Section: Latencymentioning

confidence: 99%

“…The CD5+ CD8+ T lymphocytes have been defined as the predominant site of EHV-1 latency [34]. Reactivation of latent EHV-1 has been observed after treatment with corticosteroids or stressful events (e.g.…”

Section: Latencymentioning

confidence: 99%

See 1 more Smart Citation

Equine Herpes Virus-1: Virus, Immunity and Vaccines

Paillot¹,

Case²,

Ross³

et al. 2008

TOVSJ

View full text Add to dashboard Cite

Equine Herpes virus-1 (EHV-1) is one of the most common respiratory pathogens of the horse. EHV-1 induces several clinical signs of disease ranging in severity, from mild respiratory distress to abortion in pregnant mares, neonatal foal death and neuropathogenic disorders. This review details some aspects of EHV-1 biology, its life cycle and pathogenicity in the natural host. Protective immunity stimulated by natural EHV-1 infection, which is short lived and depends of both humoral and cellular immune responses, will also be treated here. Vaccination remains today one of the best options to fight EHV-1 infection and several different strategies of vaccination that have been investigated and developed over the past decades will be presented in this report.

show abstract