In vitro protection of umbilical cord blood–derived primitive hematopoietic stem progenitor cell pool by mannose‐specific lectins via antioxidant mechanisms

Hinge, Ashwini; Limaye, Lalita; Surolia, Avadhesha; Kale, Vaijayanti

doi:10.1111/j.1537-2995.2010.02647.x

Cited by 7 publications

(7 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These in vitro effects have been described for other lectins belonging to tarin family, the GNA-related lectins, such as those found in banana, artocarpin, garlic, and dolichos. These lectins are able to promote the maintenance of human and murine cord blood C34 + cells, in vivo and in vitro [ 11 , 12 ]. Studies have demonstrated the interaction of these lectins with stromal and CD34 + cells in a carbohydrate-dependent manner and the subsequent reduction of reactive oxygen species (ROS) level as a putative molecular mechanism for the maintenance of CD34 + cells [ 11 , 12 ].…”

Section: Discussionmentioning

confidence: 99%

Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice

et al. 2018

View full text Add to dashboard Cite

Chemotherapeutic drugs, such as cyclophosphamide, cause severe immunosuppression and patients become susceptible to infections. Based on this, the immunomodulatory potential of tarin, a lectin from Colocasia esculenta, was evaluated in bone marrow cell cultures and in cyclophosphamide-immunosuppressed mice. Tarin promoted maintenance of hematopoietic progenitors and repopulation of Gr1 cells in vitro which was supported by in vivo results. In immunosuppressed mice, tarin increased bone marrow cell numbers and altered cell profile distribution by enhancing the frequency of Gr1+ progenitors, including Ly6-CintLy6-Glo, and anticipating their proliferation/differentiation in mature cells, especially Ly6-CloLy6-Ghi. Bone marrow cells harvested from tarin-treated immunosuppressed mice proliferated in response to GM-CSF or G-CSF in vitro and, the low numbers of bone marrow cells in the G0 phase, combined with a high number cells undergoing apoptosis confirmed that tarin promoted a faster and intense proliferation/differentiation, even in the presence of CY-induced toxicity. As a result, tarin minimized leukopenia in immunosuppressed mice promoting a faster recovery of peripheral leucocytes and protected erythroid bone marrow cells from CY-cytotoxicity in a dose-dependent manner. Data suggest that tarin could be considered a potential adjuvant to decrease leukopenia and possibly ameliorate anemia, if carefully evaluated in human cancer cell lineages and in clinical trials.

show abstract

Section: Discussionmentioning

confidence: 99%

Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice

et al. 2018

View full text Add to dashboard Cite

show abstract

“…37 Similarly, Hinge et al showed that the mannose-containing carbohydrate moieties present on the hematopoietic stem and progenitor cells were recognized by mannose-binding lectins and played an importantrole in the HSPC-protecting effect of these lectins. 38 The protective effect of mannose-binding lectins has also been demonstrated by Li et al, where the effect of a mannose-binding lectin, NTL, purified from Narcissus tazetta var. chinensis, prolonged the maintenance and expansion of cord blood CD34+ cells.…”

Section: Discussionmentioning

confidence: 74%

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Similarly, Hinge and coworkers, showed that the mannose-containing carbohydrate moieties present on the hematopoietic stem and progenitor cells were recognized by mannose-binding lectins and played an important role in the HSPC-protecting effect of these lectins (Hinge et al, 2010). The protective effect of mannose-binding lectins has also been demonstrated by Li and coworkers where the effect of a mannose-binding lectin, NTL, purified from Narcissus tazetta var.…”

Section: Discussionmentioning

confidence: 78%

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Jezierska-Woźniak

Lipiński

Grabarczyk

et al. 2017

Preprint

View full text Add to dashboard Cite

The objective of our study was to develop novel techniques for investigations of cell motility, and to assess whether the electric field of the therapeutic spinal cord stimulation system used in vivo contributes to the migration of human mesenchymal stem cells (hMSCs) in vitro.We have investigated electrotaxis of bone marrow-derived MSCs using pulsed electric field (PEF) in range 16-80 mV/mm and frequency 130 Hz and 240 Hz. The PEF-related dynamics of the cell surface glycosylation was evaluated using six plant lectins.PEF at physiological levels (10mV/mm; 130 Hz) did not influence cellular motility in vitro, what may correspond to the maintenance of the transplanted cells at the lesion site in vivo. Increase of the PEF intensity and frequency above physiological levels resulted in the increase in the cellular migration rate in vitro. PEF elevated above physiological intensity and frequency (40-80 mV/mm; 240 Hz), but not at physiological levels, resulted in changes of the cell surface glycosylation.We find the described approach as convenient for investigations and for the in vitro modeling of the cellular systems intended for the regenerative cell transplantations in vivo. Probing cell surface glycomes may provide valuable biomarkers to assess competence of transplanted cells.

show abstract

In vitro protection of umbilical cord blood–derived primitive hematopoietic stem progenitor cell pool by mannose‐specific lectins via antioxidant mechanisms

Abstract: The data show that both BL and GL protect the primitive HSPCs in vitro and may also serve as cost-effective HSPC enrichment tools.

Cited by 7 publications

References 40 publications

Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice

Tarin stimulates granulocyte growth in bone marrow cell cultures and minimizes immunosuppression by cyclo-phosphamide in mice

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Migration of human mesenchymal stem cells stimulated with pulsed electric field and the dynamics of the cell surface glycosylation

Contact Info

Product

Resources

About