In vitro propagation ofAtractylodes lancea

Hiraoka, Noboru; Yamada, Nobuyuki; Kodama, Tomoko; Tsutsumi, Yutaka

doi:10.1007/bf02441005

Cited by 9 publications

(4 citation statements)

References 2 publications

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“…In vitro techniques are used in conservation of rare and endangered species (Fay, 1992). Many important Chinese medicinal herbs have been successfully propagated either by organogenesis (Erdei et al, 1981;Hiraoka et al, 1984;Hatano et al, 1986;Matsumoto et al, 1986;Hiraoka and Oyanagi, 1988;Nishioka, 1988;Tsay et al, 1989) or by somatic embryogenesis (Hiraoka et al, 1983;Kitamura et al, 1989;Tsay and Huang, 1998). To date, however, there has been no report of the in vitro propagation of L. wrightii.…”

Section: Introductionmentioning

confidence: 96%

In vitro propagation of limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

Huang¹,

Hsieh²,

Hsieh³

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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Rapid in vitro propagation of Limonium wrightii (Hance) Ktze. (Plumbaginaceae), an endangered medicinal plant, was achieved by culturing the shoot-tip (primary and lateral), leaf-and inflorescence-node explants. MS (Murashige and Skoog, 1962) medium supplemented with 8.87 mM N 6 -benzyladenine (BA) and 1.07 mM a-naphthaleneacetic acid (NAA) supported induction of adventitious shoots from the shoot-tip, inflorescence-node and middle and basal parts of leaf explants after 60 d of culture. Adventitious shoots were multiplied by subculturing on MS medium supplemented with BA (2.21-17.75 mM) in combination with NAA (1.07 mM). The percentage of explants forming shoots and the average number of adventitious shoot buds produced per explant were stimulated by increasing the strength (1/4Â, 1/2Â, 1Â, 2Â) of the MS medium. Shoots were rooted on MS basal medium with 4.92 mM indole-3-butyric acid. Plantlets with a morphologically normal appearance produced from adventitious shoots were transferred to soil and acclimated in the growth chamber for 1 mo.

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Section: Introductionmentioning

confidence: 96%

In vitro propagation of limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

Huang¹,

Hsieh²,

Hsieh³

et al. 2000

In Vitro Cell.Dev.Biol.-Plant

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“…Herein, we indicate that the β-eudesmol/hinesol content ratio in A. lancea rhizomes is highly dependent on genetic factors and suggest that clonal propagation is effective for stabilizing this ratio in A. lancea. A. lancea tissues have been propagated in vitro, particularly tip tissues have been cultured extensively [23]. In particular, Shoyama et al [24] indicated that 6-benzylaminopurine facilitates shoot propagation, and gibberellin stimulates root enlargement in A. lancea.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of heritability of β-eudesmol/hinesol content ratio in Atractylodes lancea De Candolle

et al. 2020

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Background: Atractylodes lancea De Candolle is a medicinal plant distributed in East Asia. Its rhizome has been used as an important crude drug in traditional Chinese and Japanese medicines for the treatment of numerous diseases and disorders. In recent years, the demand for mass production of the crude drug with a stable quality has increased. Its major active compounds are sesquiterpenoids, such as β-eudesmol and hinesol that have closely related chemical structures with each other. As the criteria for evaluating the quality of A. lancea, the β-eudesmol/hinesol content ratio is considered important. In A. lancea, the ratio could be considered to be influenced by genetic factors, geographical environment factors and these interactions. Few studies of a detail genetic analyses for β-eudesmol/hinesol content ratio have been reported. Therefore, we evaluated the heritability and genotype-environment interaction on the βeudesmol/hinesol content ratio in A. lancea using clonal lines propagated with division of rhizome. Results: The heritability of the β-eudesmol/hinesol content ratio in A. lancea was evaluated through the cultivation of clonal lines of A. lancea in both different years (2016, 2017) and locations (Hokkaido, Ibaraki). Correlations between βeudesmol and hinesol contents were identified in all clonal lines, with high correlation coefficients (r = 0.73-0.99). The broad-sense heritability of the β-eudesmol/hinesol content ratio was revealed to be high at 0.92. The effects of cultivation year were smaller than that of genotype, and few genotype-environment interactions were observed. In addition, the influence of cultivation location was also smaller than that of genotype, and the correlation between the two cultivation locations on the β-eudesmol/hinesol content ratio was high. The results suggested that the βeudesmol/hinesol content ratio in A. lancea is highly dependent on genetic factors. Conclusion: We demonstrate that the heritability of β-eudesmol/hinesol content ratio is high and that the effects of genetic factors were stronger than that of environmental factors such as cultivation location and year. Our findings suggested that selective breeding and clonal propagation are effective strategies for the production of A. lancea with stable qualities for use in the production of crude drugs.

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“…To date, tissue culture studies of A. macrocephala have mainly focused on the plant regeneration system, including clonal multiplication of Atractylodes lancea by shoot tip culture [10] and the effect of plant growth regulators on plant regeneration from the auxiliary bud of the Atractylodes species [1,11]. However, there are only a few studies published that treat the establishment of a hairy roots culture system using leaf explants of A. japonica [12].…”

Section: Introductionmentioning

confidence: 99%

Metabolic Discrimination between Adventitious Roots and Standard Medicinal Part of Atractylodes macrocephala Koidz. Using FT-IR Spectroscopy

Choi

Ahn

et al. 2023

Plants

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This study aims to examine the metabolic discrimination between in vitro grown adventitious roots and the standard medicinal parts of Atractylodes macrocephala. To achieve this goal, firstly, in vitro culture conditions of adventitious roots such as indole-3-butyric acid (IBA) concentrations, types of media, inorganic salt strength of culture medium, and elicitor types and concentrations were optimized. The optimal culture conditions for proliferation of adventitious roots was found to consist of Murashige and Skoog (MS) medium containing 5 mg L−1 IBA. Whole cell extracts from adventitious roots and the standard medicinal parts of A. macrocephala were subjected to Fourier transform infrared spectroscopy (FT-IR). Principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) from FT-IR spectral data showed that adventitious roots and standard medicinal parts were clearly distinguished in the PCA and PLS-DA score plot. Furthermore, the overall metabolite pattern from adventitious roots was changed depending on the dose-dependent manner of chemicals. These results suggest that FT-IR spectroscopy can be applied as an alternative tool for the screening of higher metabolic root lines and for discriminating metabolic similarity between in vitro grown adventitious roots and the standard medicinal parts. In addition, the adventitious roots proliferation system established in this study can be directly applied as an alternative means for the commercial production of A. macrocephala.

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In vitro propagation ofAtractylodes lancea

Cited by 9 publications

References 2 publications

In vitro propagation of limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

In vitro propagation of limonium wrightii (Hance) Ktze. (Plumbaginaceae), an ethnomedicinal plant, from shoot-tip, leaf- and inflorescence-node explants

Evaluation of heritability of β-eudesmol/hinesol content ratio in Atractylodes lancea De Candolle

Metabolic Discrimination between Adventitious Roots and Standard Medicinal Part of Atractylodes macrocephala Koidz. Using FT-IR Spectroscopy

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