In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil)

Pattnaik, Swapnajit; Chand, Pradeep K.

doi:10.1007/s002990050133

Cited by 34 publications

(39 citation statements)

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“…Contrary to an earlier report (Ismail et al 2011), the lowest regeneration frequency with the lowest number of shoots per explant (1.2±0.37) was obtained in MS medium supplemented with 2.5 μM TDZ using nodal segments in the present study (Table 2). Reduction in the number of shoots generated from each explant at TDZ concentration higher than optimal level has also been reported for several plant species like Adhatoda beddomei (Sudha and Seeni 1994); Ocimum americanum (Pattnaik and Chand 1996) and Cassia angustifolia (Siddique and Anis 2007).…”

Section: Effect Of Thidiazuronsupporting

confidence: 55%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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A comparative performance of two explants types (CN and Nodal) for their efficiency to induce multiple shoot regeneration in Clitoria ternatea has been carried out. Thidiazuron (TDZ) in different concentrations (0.05-2.5 μM) was used as a supplement to the Murashige and Skoog's (MS) basal media. Explant type apart, two factors viz. concentration and exposure duration to TDZ played an important role in affecting multiple shoot regeneration. Cotyledonary node explants produced the best results at 0.1 μM TDZ, while in nodal explants the highest rate of shoot formation was achieved on MS medium supplemented with 1.0 μM TDZ. In both the explants, shoot multiplication increased when the regenerated shoots were subcultured on hormone free MS medium after 4 weeks of exposure to TDZ. Among the two, cotyledonary node explants produced considerably higher number of shoots at a comparatively lower concentration of TDZ than nodal explants. The regenerated shoots rooted best on MS medium containing 1.0 μM indole-3-butyric acid (IBA) and were successfully established in pots containing garden soil with 88 % survival rate. All the regenerated plants showed normal morphology and growth characteristics.

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Section: Effect Of Thidiazuronsupporting

confidence: 55%

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

Mukhtar

Ahmad²,

Khan³

et al. 2012

Physiol Mol Biol Plants

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“…Maximum number of roots (11.99 ± 0.99) were produced in 1.5 mg/l IBA and mean root length was found to be 7.60 ± 1.07 cm (Table 2). Similar results were also reported in Ocimum amaricannum (Pathnaik and Chand 1996), Hybanthus enneaspermus (Natarajan et al 1999), Tylophora asthmatica (Usha et al 2002). Twenty microshoots were used for each treatment and data (Mean ± Sd) collected after four -five weeks of culture.…”

Section: Resultssupporting

confidence: 78%

In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

Raja

Arockiasamy

2009

Plant Tissue Cult. & Biotech.

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A simple micropropagation method from nodal and shoot tip explants is reported here for Mentha viridis, an economically important medicinal plant. High frequency of microshoots was obtained from these two explants on MS supplemented with various concentrations of BAP (1.0 -4.0 mg/l) and Kn (1.0 -4.0 mg/l). Maximum number of shoots was obtained from nodal explants in the medium containing 3.0 mg/l BAP. The root induction was carried out by using IBA and IAA (0.5 -2.0 mg/l). Among these, high frequency of root proliferation was achieved in the medium containing 1.5 mg/l of IBA. The rooted plantlets were hardened and transferred to the field. The survival rate was 90 -95% after 25 days.

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“…Initially the roots were produced as a cluster from the basal cut end and gradually develop into thick and healthy roots. Similar results were obtained in S. dulcis (Rashid et al 2009) and Ocimum sanctum (Pattnaik and Chand 1996;Shahzad and Siddiqui 2000). The microshoots obtained from BA (0.5 mM) develop normal and healthy roots while the microshoots excised from Kn (1.0 mM) develop inflorescence on the rooting medium and roots were originated in clusters which did not attain sufficient length ( Figure 1D, E).…”

Section: In Vitro Rootingsupporting

confidence: 77%

Development of a regeneration system via nodal segment culture inVeronica anagallis-aquaticaL. – an amphibious medicinal plant

Shahzad

Parveen

Fatema

2011

Journal of Plant Interactions

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The present study describes an effective, reproducible and rapid regeneration protocol for in vitro shoot development of the medicinally important, semi-aquatic plant Veronica anagallis-aquatica L. through mature nodal segments on Murashige and Skoog (MS) medium. The most effective medium for shoot regeneration was MS medium fortified with 0.5 mM 6-benzyladenine (BA), on which the maximum number (43.791.85) of shoots per explant having a shoot length of 5.090.25 cm were produced. The addition of auxins along with optimal concentration (0.5 mM) of BA did not further improve the regeneration capacity of the explants; instead, it resulted in an adverse effect. Nodal segments cultured in MS liquid medium without agar provided a better and early response for efficient shoot regeneration, and simultaneous rooting as compared to solidified medium. The optimal liquid medium comprised of MS'BA (0.5 mM) on which a maximum 20.791.76 shoots per explant were produced and grew healthier. Both half strength MS and MS basal medium supplemented with anaphthaleneacetic acid (NAA) responded equally for root induction. Rooted shoots were successfully transferred to field conditions with 80% survival. Histological studies revealed the de novo origin of shoots which would make this protocol suitable for transformation techniques.

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In vitro propagation of the medicinal herbs Ocimum americanum L. syn. O. canum Sims. (hoary basil) and Ocimum sanctum L. (holy basil)

Cited by 34 publications

References 0 publications

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

RETRACTED ARTICLE: Influencing micropropagation in Clitoria ternatea L. through the manipulation of TDZ levels and use of different explant types

In vitro Propagation of Mentha viridis L. from Nodal and Shoot tip Explants

Development of a regeneration system via nodal segment culture inVeronica anagallis-aquaticaL. – an amphibious medicinal plant

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