In vitro propagation of the medicinal plant Plumbago zeylanica L. Through nodal explants

Abstract

A protocol for rapid in vitro propagation using nodal explants obtained from 2-yr-old, field-grown medicinal plants of Plumbago zeylanica L. belonging to the family Plumbaginaceae is described. High frequency bud break and fast development of shoots were induced on Murashige and Skoog's basal medium supplemented with 27.2 mM adenine sulfate +2.46 mM indole-3-butyric acid (IBA). Induction of rooting was achieved by transferring the shoots to the same basal medium containing 4.92 mM IBA. Using our protocol from one twig of P. zeylanica (eight responsive nodes per explant shoot) within a period of 5 mo., eight plantlets could be raised. After a hardening period of 4 wk, there was a 90% transplantation success in the field compared to the 60±65% survival of plantlets recorded in the experiments of previous workers. The plantlets derived through in vitro propagation mimic the growth and morphological characteristics of the donor plants.

In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

“…Sahoo and Debata (1998) reported that a rapid and highly effective method for plant micropropagation from vegetative shoot buds was established for this medicinal plant Plumbago zeylanica L. ; multiple shoots were proliferated from nodal explants culture on MS basal medium supplemented with 0.25-1.0 mg/l Kn or 0.25-1.0 mg/l BAP. A similar phenomenon was observed in Plumbago zeylanica L. by other researchers (Gbadamosi and Egunyomi, 2010;Mallikadevi et al, 2008;Selvakumar et al, 2001;Rout et al, 1999;Verma et al, 2002).…”

In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

“…1d) when cultured individually on root induction medium consisted of halfstrength MS medium with 0.5 mg/l IAA (Table II). Use of auxins singly or in combination for rooting was also reported by different authors (Gbadamosi and Egunyomi, 2010;Mallikadevi et al, 2008;Hassan and Khatun, 2010;Selvakumar et al, 2001;Rout et al, 1999;Verma et al, 2002).…”

<i>In vitro</i> propagation of <i>Aegle marmelos</i> (L.) corr., a medicinal plant through axillary bud multiplication

“…In general, micropropagation through axillary shoot proliferation has proven to be a handy tool [11] An appreciable level of success has been achieved by many investigators in the case of many tropical medicinal plants through micropropagation as in case of Hemidesmus indicus ([12], Cleistanthus collinus [13], Gmelina arborea [14], Plumbago zeylanica [15] and Holarrhena antidysenterica [16]. Although there are techniques have been reported for in vitro propagation of Aegle marmelos from in vitro grown seedling parts, i.e.…”