In vitro propagation of the medicinal plant Ziziphora tenuior L. and evaluation of its antioxidant activity

Abstract

Ziziphora tenuior L. (Lamiaceae) is an aromatic herb used for its medicinal values against fungi, bacteria. Micropropagation can be used for large-scale multiplication of essential oil producing plants thus avoiding an overexploitation of natural resources. This work aims to develop a reliable protocol for the in vitro propagation of Z. tenuior, and to compare the antioxidant activity between in vitro propagated and wild plants. The explants were sterilized and cultured on MS medium containing different concentrations of growth regulators naphthalene acetic acid (NAA) or indole-3-butyric acid (IBA) with 0.5 mg/L of kinetin (Kin) callus formation was 70.2% after 45 days of incubation in dark on medium supplemented with 1.5 mg/L of NAA. After one month of callus culture on medium supplemented with 2 mg/L BA the shoot number was 5.12 and for the multiplication stage. The shoot number was 4.21 and length was 6.17 cm on medium supplemented with 1 mg/L Kin + 0.1 mg/L NAA. DPPH• reagent was used to test the antioxidant activity. The aqueous and methanol extracts of in vitro plants which were treated with 1.5 and 1 mg/L of kin plus 0.1 mg/L of NAA showed a strong DPPH• scavenging activity where IC50 was 0.307 and 0.369 mg/ml, respectively, while the IC50 of aqueous and methanol extracts of wild plants was 0.516 and 9.229 mg/ml, respectively. Our results suggested that plant growth regulators and in vitro culture conditions increased the antioxidant activity.

Mass propagation of Plectranthus bourneae Gamble through indirect organogenesis from leaf and internode explants

“…Means followed by the same letters in each column are not significantly different (P = 0.05) using Duncan's multiple range test 1.0 mg/l in combination with 0.7 mg/l NAA responded highest shoot regeneration (89 %) with highest number of shoots (25.57) and shoot length (3.87) on leaf derived callus (Table 3). Similar results have also been observed in leaf explants of in Peperomia obtusifolia (Naggar and Osman 2014) and Ziziphora tenuior (Dakah et al 2014). Likewise, internodal explants cultured on MS medium supplemented with KN (1.0 mg/l) and NAA (0.7 mg/l) showed higher regeneration frequency (85 %) and highest number (16.42) of shoots and shoot length (2.94).…”

Micropropagation of Agave salmiana: Means to Production of Antioxidant and Bioactive Principles

“…The phytochemicals found in Agave include phenolic compounds, such as kaempferol and quercetin, in various glycosylated forms (Almaráz-Abarca et al, 2013; Barriada-Bernal et al, 2014), and saponins, as glycosides of hecogenin, diosgenin, chlorogenin, kammogenin, and gentrogenin (Yokosuka and Mimaki, 2007; Pérez et al, 2014). Previous studies have reported an increase in bioactivity of diverse micropropagated plants compared to wild-type (WT) plants (García-Pérez et al, 2012; Dakah et al, 2014), suggesting that the micropropagation of A. salmiana could result in this advantage.…”

Polyphenol profile and antioxidant activity of extracts from olive leaves

“…The differences in the reported results can be explained by genotypic characteristics of the cultivars and different age, nutritional supplement, the effect of growth regulators and culture conditions in vitro. This dependence has been observed by Zayova et al (2012) and Dakah et al (2014) studying the antioxidant ability of the in vitro and in vivo cultures of medicinal plants.…”