In vitro propagation of Sandersonia aurantiaca Hook using thidiazuron

Deroles, Simon C.; Seelye, J.F.; Javellana, John; Mullan, A. C.

doi:10.1007/s11240-010-9705-9

Cited by 13 publications

(6 citation statements)

References 10 publications

(13 reference statements)

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“…However, this extremely high shoot proliferation rate was achieved with germinating zygotic embryos, which were recombinant genotypes, and the attributes of a mature tree originating from such material are difficult to judge. The effective use of TDZ as the sole growth regulating compound for adventitious shoot regeneration was reported in several plant species such as Sandersonia aurantiaca (Deroles et al 2010), Phyllanthus amarus (Nitnaware et al 2011), Ochna integerrima (Ma et al 2011a) and also in Viburnum dentatum where its use was supplemented with dark treatments (Dai et al 2011). In our experiments we found TDZ to be an inconvenient plant growth regulator which frequently induced the formation of fasciated shoots.…”

Section: Discussionmentioning

confidence: 50%

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

Ďurkovič

Kaňuchová

Kačík

et al. 2011

Plant Cell Tiss Organ Cult

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Proliferating shoot cultures of black mulberry (Morus nigra), derived from axillary buds of two donor trees designated as Mn1 and Mn2, more than 80 years of age, were established in vitro. Subsequently, shoot-tips were used to induce both axillary and adventitious shoot regeneration following incubation on Murashige and Skoog medium containing 14 different treatments of various concentrations of plant growth regulators, including 6-benzyladenine (BA), thidiazuron (TDZ), and combinations of BA with indole-3-butyric acid (IBA) and TDZ with BA. The highest shoot proliferation of 5.3 shoots per explant for the Mn1 tree and 6.9 shoots per explant for the Mn2 tree were obtained when explants were incubated on a medium containing 0.5 mg l -1 BA and 0.1 mg l -1 IBA. The maximum frequency of adventitious rooting was similar for both genotypes. Changes in lignin and cellulose content, macromolecular properties of dioxane and Klason lignins, lignin monomer composition, and macromolecular properties of cellulose were determined in 1-year-old and 3 year-old micropropagated plants, as well as in the donor trees. Lignin and cellulose properties were significantly dependent on the genotype, the age and the mutual interaction of both these factors. The syringyl to guaiacyl weight ratio in lignin rose with the age of the micropropagated plants. Moreover, the tensile strength of wood in 1-year-old plants was supported by a high cellulose degree of polymerization. The highest polydispersity index of cellulose was detected in 3-year-old plants.

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Section: Discussionmentioning

confidence: 50%

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

Ďurkovič

Kaňuchová

Kačík

et al. 2011

Plant Cell Tiss Organ Cult

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“…Thidizazuron (TDZ), a new synthetic cytokinin-like PGR, has been reported to induce high-frequency somatic embryogenesis and organogenesis in dicots, such as Petunia hybrida (Thirukkumaran et al 2009), Phaseolus vulgaris (Kwapata et al 2010), and Phyllanthus amarus (Nitnaware et al 2011). It has also been reported recently that TDZ is effective in inducing somatic embryogenesis and organogenesis in monocots (Shan et al 2000;Gari and Rashid 2004;Panaia et al 2004;Ganeshan et al 2006;Cheruvathur et al 2010;Deroles et al 2010). Other factors in addition to embryogenic callus induction and regeneration also affect transformation frequency, including Agrobacterium strain, antibiotics used for controlling agrobacteria growth, and plant regeneration from resistant calli.…”

Section: Introductionmentioning

confidence: 99%

An efficient regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297

Zhao

Zheng

Ling

2011

Plant Cell Tiss Organ Cult

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A highly efficient tissue culture system and Agrobacterium-mediated transformation protocol for Chinese upland rice cultivar Handao297 has been established with mature embryos as explants. Up to 81.2% of mature embryos were induced to regenerate good-quality calli on NB medium (a medium combining N6 macronutrient components and B5 micronutrient and organic components) containing 3 mg/l 2,4-dichlorophenoxyacetic acid in 10 days. More than 80% of the calli were morphogenic within 1 week and regenerated green plantlets within 1 month on Murashige and Skoog medium supplemented with 0.5 mg/l 6-benzyladenine, 0.5 mg/l kinetin, 1 mg/l zeatin, 0.5 mg/l thidizazuron (TDZ), 0.5 mg/l naphthaleneacetic acid, 0.15 mg/l indoleacetic acid, and 0.15 mg/l indolebutyric acid. This tissue culture system was suitable for Agrobacterium-mediated transformation of upland rice Handao297. Furthermore, some important factors affecting transformation frequency were investigated with Agrobacterium strain AGL1 containing the plasmid pCAM-BIA1381. The addition of 30 mg/l hygromycin B followed by 60 mg/l hygromycin B to the selection induction medium facilitated the revival of calli from selection and reduced false positive calli. Hygromycin B at 10 mg/l was most effective in suppressing non-transgenic callus growth in the differentiation medium. The addition of TDZ to the differentiation medium promoted the morphogenesis of calli and facilitated the generation of adventitious shoots by five to tenfold in comparison to medium without TDZ.

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“…Estudios realizados con plantas de Dioscorea cayenensis y D. rotundada evidenciaron la correlación entre los altos niveles de sacarosa (8%) con diferentes niveles de ácido jasmónico (JA) para aumentar la inducción de microtúberos en condiciones in vitro, lo cual ratifica efecto potencializador de la sacarosa en presencia del regulador de crecimiento para mejorar los niveles de inducción (Olivier et al 2012;Dai et al 2014). El efecto inductor de reguladores de crecimiento en la formación de órganos de reserva y de propagación en condiciones in vitro en combinación o ausencia de sacarosa ha sido reportado en otras especies vegetales (Deroles et al 2010;Rayirath et al 2011;López 2015).…”

Section: Inducción De Microtúberosunclassified

Ácido abscísico y sacarosa afectan la formación in vitro de túberos en plantas de ñame (Dioscorea rotundata Poir.)

Padrón

Otero

2016

Temas Agrarios

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El túbero de ñame (Dioscorea rotundata Poir.) es una de las principales fuentes alimenticias de los pobladores de la Costa Atlántica Colombiana y la cuenca del Caribe, y su demanda ha aumentado en el mercado internacional en los últimos años; sin embargo, las técnicas convencionales de cultivo afectan la cantidad y calidad de la producción. Con el fin de ofrecer mecanismos de producción de material de siembra de ñame de buena calidad, se evaluó el efecto de tres concentraciones de sacarosa y cuatro niveles de ácido abscísico en la producción in vitro de microtúberos a partir de explantes nodales. Los tratamientos se distribuyeron con un diseño completamente al azar con ocho repeticiones por tratamiento. El análisis de varianza y la prueba de media de Duncan indicaron que tanto las variables del crecimiento de las plantas como el número de microtúberos mostraron diferencias significativas (Pr<0,05) como resultado de los niveles de ABA/sacarosa, una dosis de 60 g L-1 de sacarosa combinada con 3,0 mg L-1 de ABA indujo el mayor número de microtúberos, mientras que las plantas cultivadas en presencia de 90 g L-1 de sacarosa formaron mayor número de tallos, hojas y raíces.

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In vitro propagation of Sandersonia aurantiaca Hook using thidiazuron

Cited by 13 publications

References 10 publications

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

Genotype- and age-dependent patterns of lignin and cellulose in regenerants derived from 80-year-old trees of black mulberry (Morus nigra L.)

An efficient regeneration system and Agrobacterium-mediated transformation of Chinese upland rice cultivar Handao297

Ácido abscísico y sacarosa afectan la formación in vitro de túberos en plantas de ñame (Dioscorea rotundata Poir.)

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