In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree

Naik, Soumendra K.; Pattnaik, Swapnajit; Chand, Pradeep K.

doi:10.1016/s0304-4238(98)00218-0

Cited by 61 publications

(33 citation statements)

References 15 publications

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“…Although tissue cultures of edible pomegranate via shoot organogenesis, somatic embryogenesis, and enhanced auxiliary bud proliferation have been reported by Omura, et al (1987) ;Naik, et al (1999Naik, et al ( , 2000; Shao, et al (2003) and Terakami, et al (2007). In this study an efficient system for callus induction, regeneration, rooting and acclimation of dwarf pomegranate is reported.…”

Section: Introductionmentioning

confidence: 78%

Callus Induction and Plant Regeneration in Punica Granatum L. ʻnanaʼ From Leaf Explants

Bonyanpour¹,

Khosh-Khui²

2013

JCEA

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In this investigation, leaf explants of a local cultivar of dwarf pomegranate were placed on Murashige and Skoog (1962) (MS) medium supplemented with various concentrations of 6-benzyl adenin (BA) and naphthalene acetic acid (NAA) for callus induction. After 40 days, maximum callus induction was observed on a media containing 1 mg L -1 BA and 0.2 to 0.4 mg L -1 NAA. However, the highest callus growth was obtained on a medium containing 1 mg L -1 BA and 1 mg L -1 NAA. The highest number of shoots (7 shoots per explants) was obtained by transferring the calli to the media containing 5 mg L -1 BA with 0.1 mg L -1 NAA. Maximum shoot proliferation was observed when shoots were cultured on woody plant medium (WPM) supplemented with 5 mg L -1 kinetin (Kin). In this treatment, after 4 subcultures, 36 shoots were produced from one original explant. Among treatments used in rooting experiments, shoots cultured on WPM medium containing 0.2 mg L -1 indol butyric acid (IBA) had the maximum root percentage (100%) and good root growth (2.06 cm mean length and 2 roots in each explants). Rooted plantlets were cultured in a soil mixture containing vermiculite (60%), perlite (30%) and coco peat (10%) v/v. After 2 months, 80% of plants survived and transferred to the greenhouse.

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Section: Introductionmentioning

confidence: 78%

Callus Induction and Plant Regeneration in Punica Granatum L. ʻnanaʼ From Leaf Explants

Bonyanpour¹,

Khosh-Khui²

2013

JCEA

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“…Protocols for regeneration of P. granatum have been reported by a number of researchers (Naik et al 1999;Naik et al 2000;Shao et al 2003;Chauhan and Kanwar 2012); however, these protocols display several problems related to establishment of plant materials and in vitro responsiveness (Chauhan and Kanwar 2012). The most commonly used protocols for organogenesis have employed seedling derived plant materials (Chauhan and Kanwar 2012), but this approach is not suitable for elite cultivars.…”

Section: Introductionmentioning

confidence: 99%

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

ValizadehKaji

Ahmad

Tohidfar

2013

Physiol Mol Biol Plants

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“…Protocols for regeneration of P. granatum via shoot organogenesis, somatic embryogenesis, and enhanced axillary bud proliferation have been reported (Omura et al 1987;Murkute et al 2002;Jaidka and Mehra 1986;Nataraja and Neelambika 1996;Naik et al 1999;Naik et al 2000;Shao et al 2003;Terakami et al 2007); however, these protocols remain inefficient. In this study, an efficient system for shoot organogenesis and somatic embryogenesis from cotyledonary and zygotic embryos of P. granatum is reported.…”

Section: Introductionmentioning

confidence: 99%

Comparison of in vitro regeneration pathways in Punica granatum L.

Kanwar

Joseph

Deepika

2009

Plant Cell Tiss Organ Cult

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When cotyledonary explants, excised from in vitro germinated seedlings, of pomegranate (Punica granatum L.) were incubated on solid Murashige and Skoog (1962) medium supplemented with 21 lM naptheleneacetic acid (NAA) and 9 lM 6-benzyladenine (BA), 80% of explants developed callus. A high frequency of shoot organogensis was obtained when explants were incubated on MS medium supplemented with 8 lM BA, 6 lM NAA, and 6 lM giberrellic acid (GA 3 ). However, adding 24 lM silver nitrate (AgNO 3 ) to this medium markedly enhanced shoot regeneration frequency (63%) and mean number of shoots per explant (11.26) and length of shoots (2.22 cm). Highest frequency of in vitro rooting, mean number of roots/shoot (4.32), and mean root length (2.71 cm) were obtained when regenerated shoots were transferred to halfstrength MS medium supplemented with 0.02% activated charcoal. Well-rooted plantlets were acclimatized, and then transferred to soil medium. Moreover, when zygotic embryos of P. granatum, excised from seeds collected at 16 weeks following full bloom, were incubated on MS medium containing 30 g l -1 sucrose, 15% coconut water, 21 lM NAA, and 9 lM BA, they developed the highest frequency of embryogenic callus, clumps with globular embryos, and mean number of both globular and heartshaped embryos per callus clump. Subjecting zygotic embryo explants to six-week dark incubation period was essential for embryogenic callus induction, and these were subsequently transferred to 16 h photoperiod for further growth and development of somatic embryos. Germination of somatic embryos was observed when these were transferred to MS medium was supplemented with 60 g l -1 sucrose.

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In vitro propagation of pomegranate (Punica granatum L. cv. Ganesh) through axillary shoot proliferation from nodal segments of mature tree

Cited by 61 publications

References 15 publications

Callus Induction and Plant Regeneration in Punica Granatum L. ʻnanaʼ From Leaf Explants

Callus Induction and Plant Regeneration in Punica Granatum L. ʻnanaʼ From Leaf Explants

In vitro propagation of two Iranian commercial pomegranates (Punica granatum L.) cvs. ‘Malas Saveh’ and ‘Yusef Khani’

Comparison of in vitro regeneration pathways in Punica granatum L.

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