In vitro propagation of Helianthemum marminorense

Serrano-Martínez, Francisco; Cano-Castillo, Miriam; Casas, José Luis

doi:10.1007/s13562-011-0102-1

Cited by 4 publications

(7 citation statements)

References 10 publications

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“…In view that the inclusion of growth regulators in the medium did not improve the morphogenic response of T. moroderi explants with respect to untreated controls, we decided to apply DPS to the regeneration of this species. We have previously described the usefulness of DPS for the in vitro development of different species, not related to Lamiaceae, such as Astragalus nitidiflorus (Leguminosae) (Cano-Castillo et al 2009) or Helianthemum marminorense (Cistaceae) (Serrano-Martínez et al 2012). Also in Thymus moroderi DPS produced better results regarding shoot proliferation and elongation than controls.…”

Section: Shoot Proliferationmentioning

confidence: 99%

“…In Spain, several species have been already preserved by means of these techniques (González-Benito et al 1999;González-Benito and Martín 2011), including endemism such as Astragalus nitidiflorus (Cano-Castillo et al 2009) or Helianthemum marminorense (Serrano-Martínez et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In the search of this objective, we have investigated the use of liquid supplements, or double-phase culture system (DPS), to enhance culture performance as described previously (Cano-Castillo et al 2009;Serrano-Martínez et al 2012). The essential oil content of micropropagated and acclimatized plants was analyzed and compared to that of wild plants in order to ensure that biosynthetic ability was not altered as a consequence of the regeneration protocol.…”

Section: Introductionmentioning

confidence: 99%

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In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Marco-Medina

Casas

2014

Plant Cell Tiss Organ Cult

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Thymus moroderi Pau ex Martínez (Lamiaceae) is a species endemic to South-eastern Spain valuable for its use in traditional medicine and in the production of a liqueur called cantueso. We describe a rapid and reliable in vitro propagation protocol for this species, starting from wild adult vegetative explants. Sterilization and establishment of in vitro culture were shown to be extremely difficult because of the high presence of contamination and the inability of non-contaminated explants to restore growth. In just two cases we were able to obtain non-contaminated explants with ability to grow: combining a fungicide dip with the presence of kanamycin in culture medium, and when mother plants were maintained for a week in a growth chamber with occasional fungicide spray. Once established in vitro, explants showed rapid and vigorous growth with spontaneous rooting. The presence of cytokinin alone or in combination with auxin gave worse results than untreated controls, thus revealing Thymus moroderi as a cytokinin-sensitive species. Better multiplication rates were however obtained when double phase system was included in the protocol. Fully acclimatized micropropagated plants were phenotypically indistinguishable from their wild relatives. Essential oils of micropropagated and wild plants were analyzed revealing a slightly different overall terpene profile. Micropropagated plants were abundant in monoterpene and sesquiterpene hydrocarbons but less in oxygenated sesquiterpenes with respect to wild plants. Major compounds were in both cases 1,8-cineole and camphor, although at different relative abundances. Despite these dissimilarities micropropagated plants produced a clearly recognizable and characteristic thyme odor.

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Section: Shoot Proliferationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Marco-Medina

Casas

2014

Plant Cell Tiss Organ Cult

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“…The shoot, node and apical parts of the plant were successfully used as an initial explant in tissue culture of Helianthemum sp. genera, as well as in many plants [24,25,26,27,28,29]. In the present study, depending on the development of explants, the media in which they were planted and the PGR combinations they contain, callus, shoot and root formations were observed.…”

Section: Resultsmentioning

confidence: 54%

“…Serrano-Martinez and coauthors in H. marminorense, Hamza and coauthors and Salih and coauthors in H. lippii L. var. sessiliforium, [26,27,29] used only MS and DKW basal media. In our study, an optimization method was applied for in vitro propagation of H. germanicopolitanum using three different basal media together with PGR combinations.…”

Section: Initial Shoot Lengthmentioning

confidence: 99%

In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm.

Kapdan

Sezgin

2021

Braz. arch. biol. technol.

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In this study, in vitro propagation and acclimatization of Helianthemum germanicopolitanum Bornm. plant, a local endemic in Çankırı Province (Turkey) with arid and semi-arid lands, and an endangered species taking part among medicinal and aromatic plants were accomplished, which is under-researched. In this study, three basal media [a) Murashige and Skoog b) Gamborg's B5, and c) Nitsch & Nitsch], two gelling agents (agar 7 g/L, and gelrite 2.1 g/L), eight cytokinins and eight auxin doses of plant growth regulators [a) 6-benzyladenin, b) Kinetin-(0, 0.5, 1, and 2 mg/L), c) Indole-3-butyric acid, d) α-napthaleneacetic acid-(0, 0.25, 0.5, and 1 mg/L)] prepared in 64 different combinations with 30 g/L sucrose was added to the basal media and adjusted to pH 5.7 for in vitro propagation of H. germanicopolitanum. During in vitro propagation of the plant, external and internal infections were frequently encountered and this was solved by the developed protocol. The best shoot growth (1.141 cm) and shoot length (0.572 cm) were obtained in the Gamborg's B5 medium in combination with Kinetin (0.5 mg/L)+Indole-3-butyric acid (0.5 mg/L)+gelrite. The maximum number of shoots (19.50) and the best multiplication rate (94%) were obtained in the media containing benzyladenin (1 mg/L)+Indole-3-butyric acid (0.5 mg/L) plant growth regulator in Murashige and Skoog medium solidified with agar. At the rooting stage, the maximum number of roots (30) was reached in the Murashige and Skoog medium containing gelrite and the best rooting rate (92%) with agar. A hundred plants representing the best shoot and root growth were taken to acclimatization stage, and 32 of these plants adapted to external conditions.

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Unveiling innovations for enhancing in vitro propagation of Vanilla phaeantha Rchb.f. through the use of double-phase technique and temporary immersion bioreactor systems

Medeiros,

Silva-Cardoso,

da Silva Costa

et al. 2024

Industrial Crops and Products

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In vitro propagation of Helianthemum marminorense

Cited by 4 publications

References 10 publications

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

In vitro Propagation to Conserve the Local Endemic and Endangered Medicinal Plant Helianthemum germanicopolitanum Bornm.

Unveiling innovations for enhancing in vitro propagation of Vanilla phaeantha Rchb.f. through the use of double-phase technique and temporary immersion bioreactor systems

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