In Vitro Propagation of Camellia oleifera Abel. Using Hypocotyl, Cotyledonary Node, and Radicle Explants

Abstract

<jats:p><jats:italic>Camellia oleifera</jats:italic> Abel. is one of four major woody oil plants in the world. The objective of the current study was to evaluate the effect of different plant growth regulators (PGRs) and concentrations on direct organogenesis using cotyledonary nodes, hypocotyls, and radicle explants. High induction frequency of adventitious shoots were obtained from cotyledonary nodes, hypocotyls, and radicle explants (85.2%, 73.6%, and 41.0%, respectively) when cultured on half-strength Murashige and Skoog (1/2 MS) medium containing 2.0 mg·L<jats:sup>−1</jats:sup> 6-benzylaminopurine (BA) and 0.1 mg·L<jats:sup>−1</jats:sup> indole-3-acetic acid (IAA). Microshoots from cotyledonary nodes, hypocotyls, and radicle explants were then transferred to 1/2 MS medium containing 2.0 mg·L<jats:sup>−1</jats:sup> BA and 0.05 mg·L<jats:sup>−1</jats:sup> indole-3-butyric acid (IBA) for shoot multiplication, resulting in 6.9 shoots per explant. The shoots were transferred to Woody Plant Medium (WPM) supplemented with various α-naphthalene acetic acid (NAA) and gibberellic acid (GA<jats:sub>3</jats:sub>) for shoot elongation. The mean length of shoots and the number of leaves per shoot were 3.7 and 6.6 cm, respectively, in WPM supplemented with 0.5 mg·L<jats:sup>−1</jats:sup> NAA and 3.0 mg·L<jats:sup>−1</jats:sup> GA<jats:sub>3</jats:sub>. The highest rooting of shoots (90.2%) or the number of roots per shoot (7.2) was obtained when elongated microshoots were transferred to 1/2 MS medium supplemented with 3.5% perlite, 1.0 mg·L<jats:sup>−1</jats:sup> IBA and 2.0 mg·L<jats:sup>−1</jats:sup> NAA. The rooted plantlets were successfully acclimatized in the greenhouse with a survival rate of 90.0%. The in vitro plant regeneration procedure described in this study is beneficial for mass propagation and improvement of <jats:italic>C. oleifera</jats:italic> through genetic engineering.</jats:p>

Optimizing sterilization conditions and growth regulator effects on in vitro shoot regeneration through direct organogenesis in Chenopodium quinoa

“…Therefore, much attention was given to the analysis of using different parts of in vitro seedlings in plant tissue cultures as possible explants. For instance, shoot tips, hypocotyls segments, embryonal axis, and axenic leaves from in vitro grown seedling were used as explants in many studies conducted by different researchers to set applicable and effective protocols for many species (Eisa et al, 2005;Daneshvar, 2016a, 2016b;Hesami et al, 2017;Hsieh et al, 2017;Jafari et al, 2017;Li et al, 2016;Mali and Chavan, 2016). This study indicated, for the first time, the usefulness of cotyledonary node segments obtained from axenic-seedlings as a source of explants for successful multiplication of quinoa ( Fig.…”

“…In organ and callus cultures, the shoot formation usually leads to genetic alterations, while genetic characteristics of clones can be preserved by propagation via shoot tips or meristem cultures (Bhojwani and Dantu, 2013). Numerous studies on different plants such as Arachis hypogaea (Hsieh et al, 2017), Moringa oleifera (Avila-Treviño et al, 2017), Camellia oleifera (Li et al, 2016), Ficus religiosa L. (Hesami et al, 2017), Passiflora caerulea L. (Jafari et al, 2017), and Withania somnifera (Nayak et al, 2013) have indi-cated that cotyledonary node explants possess a great potential for shoot proliferation. However, to date there have been limited studies on applying in vitro methods on plants from Chenopodiaceae family.…”

Transcriptomic Analyses of Camellia oleifera ‘Huaxin’ Leaf Reveal Candidate Genes Related to Long-Term Cold Stress

“…is a member of the genus Camellia in the family Theaceae. In China, cultivated C. oleifera is one of the four major woody oil species, followed by the tung tree (Vernicia fordii Hemsley), walnut (Juglansregia), and Chinese tallow tree (Sapium sebiferum) [1,2]. The tea oil extracted from C. oleifera seeds is an edible oil known as "eastern olive oil," because of its high nutritional value and health benefits [3].…”

Light quality affects the proliferation of in vitro cultured plantlets of Camellia oleifera Huajin

“…Camellia oleifera (family, Theaceae) is an important oil-yielding, woody evergreen shrub or small tree native to China ( Gao, Yang & Yuan, 2017 ). The four major trees producing edible oil in the world are C. oleifera , olive ( Olea europaea ), oil palm ( Elaeis guineensis ), and coconut ( Cocos nucifera ) ( Li et al, 2016 ). The main product of C. oleifera is tea oil, also known as ‘Oriental olive oil’, which is highly recommended as a healthy edible oil by the International Food and Agriculture Organization.…”

“…The main product of C. oleifera is tea oil, also known as ‘Oriental olive oil’, which is highly recommended as a healthy edible oil by the International Food and Agriculture Organization. It is rich in unsaturated fatty acids, squalene, vitamin E, and other nutrients ( Li et al, 2016 ). Tea oil is known to decrease lipid concentrations and prevent hypertension and the hardening of arteries ( Li et al, 2016 ).…”