In vitro propagation of blue honeysuckle

Sedlák, J.; Paprštein, F.

doi:10.17221/1871-hortsci

Cited by 15 publications

(24 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was probably due to the toxicity of used concentration of mercuric chloride to plant tissues of two selected sweet cherry cultivars. It was previously reported that mercuric chloride is a good disinfectant, but could be toxic to plant tissues of susceptible species and genotypes (Muna et al 1999;Sedlák, Paprštein 2007).…”

Section: Resultsmentioning

confidence: 99%

In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

Sedlák¹,

Paprštein²

2008

Hort. Sci. (Prague)

Self Cite

View full text Add to dashboard Cite

abstract:The objective of this study was to investigate the possibility of optimizing routine tissue culture methods to proliferate two sweet cherry cultivars Karešova and Rivan. Shoot tips of two genotypes were successfully established in vitro. Six proliferation MS media containing 1, 2 and 4 mg/l BAP (6-benzylaminopurine), 0.5 and 1 mg/l TDZ (thidiazuron) or 10 mg/l 2iP (6-(γ,γ-dimethylallylamino)purine) were tested. The highest proliferation rate (3.0 ± 0.1) was obtained for Rivan on MS medium containing 2 mg/l BAP. In the case of cultivar Karešova, any of the cytokinins tested did not promote satisfactory proliferation. The highest proliferation rate (1.6) achieved on MS medium with 2 mg/l 2iP is not sufficient for a larger scale in vitro shoot production. It was proved that different genotypes of sweet cherry do not respond in the same way during proliferation in vitro. Future research and testing of other media and plant growth regulators will be carried out.

show abstract

Section: Resultsmentioning

confidence: 99%

In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

Sedlák¹,

Paprštein²

2008

Hort. Sci. (Prague)

Self Cite

View full text Add to dashboard Cite

show abstract

“…For disinfection the initial explants, chemical solutions such as mercuric chloride (Sedlák and Paprstein 2007), mercuric sulfate (Krupa-Małkiewicz and Ochmian 2014), sodium hypochloride (Karhu 1997;Osburn et al 2009), and calcium hypochloride (Dziedzic 2008) are used. In our study, two disinfectants, mercuric sulfate and sodium hypochloride were applied.…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…These explants were later discarded. Sedlák and Paprstein (2007) used 0.15% mercuric chloride (HgCl2) solution for disinfection and observed that 50% of initial uncontaminated explants of cultivar Altaj died after the sterilization procedure. According to Debnath (2007) the most important and necessary step in vitro propagation of Vaccinium genus is regeneration from primary explants.…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…The shoot length of blue honeysuckle on MS media supplemented with 1.0 and 4.0 mg • dm -3 was similar to that of the control group (47.50 mm), while the explants on MS medium supplemented with BAP at a concentration of 2.0 mg • dm -3 were slightly lower (33.80 mm). According to Karhu (1997), Sedlák and Paprstein (2007), Dziedzic (2008) as well Krupa--Małkiewicz and Ochmian (2014) the best initiation medium to obtain the largest number of new microshoots is MS supplemented with BAP at concentrations of 1.0 and 2.0 mg • dm -3 . Osburn et al (2009) for the initiation of Japanese and 'Amur' honeysuckle applied three kinds of media: DKW (Driver and Kuniyuki 1984), MS, and WPM (Lloyd and McCown 1981).…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…The tissue culture techniques can be advantageous for the preservation of sufficiently large range of plant variation for future breeding programs. The effectiveness of micropropagation of Lonicera species depends on media and PGRs (Plant Growth Regulators), and is genotype specific (Debnath 2007;Sedlák and Paprstein 2007;Hui et al 2012;Krupa-Małkiewicz and Ochmian 2014).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

COMPARISON OF PROPAGATION METHOD IN IN VITRO AND IN VIVO CONDITION OF Lonicera caerulea L.

Krupa-Małkiewicz¹,

Ochmian²,

Smolik³

et al. 2017

Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Z

View full text Add to dashboard Cite

Streszczenie. Celem badań było określenie efektywności ukorzeniania jagody kamczackiej 'Wojtek' w kulturach in vitro. Ponadto określono wpływ sposobu cięcia sadzonek zdrewniałych i zielnych na ukorzenianie w różnych podłożach w warunkach in vivo. Decydującym etapem przygotowania eksplantatów do namnażania jest etap dezynfekcji. Jeśli chodzi o użyte w doświadczeniu środki dezynfekujące, najlepsze efekty uzyskano po zastosowaniu 10% podchlorynu sodu (NaOCl). Najlepszą pożywka do inicjacji i namnażania eksplantatów pędowych jagody kamczackiej 'Wojtek' była pożywka według składu Murashige i Skoog (MS). Natomiast nie zaobserwowano pozytywnego wpływu cytokininy BAP na proces inicjacji i namnażania pędów in vitro. Brak roślinnych regulatorów wzrostu w pożywce ukorzeniającej miał hamujący wpływ na procent ukorzenionych roślin jagody kamczackiej. Natomiast dodatek auksyny IBA do pożywki MS dodatnio wpływał na wysokość roślin, długość i liczbę korzeni. W warunkach szklarniowych największy procent ukorzenionych roślin otrzymano z 4-pędowych zdrewniałych sadzonek. Najlepszym podłożem do ukorzeniania okazał się perlit w połączeniu z torfem. Najgorsze efekty obserwowano w przypadku pędów ukorzenianych w piasku.

show abstract

The influence of methods of obtaining planting material on the formation of productivity in blue honeysuckle plantations

Bryksin

2021

BIO Web Conf.

View full text Add to dashboard Cite

Blue honeysuckle has successfully become one of the industrial berry crops of the XXI century. The area of blue honeysuckle plantations in Russia exceeds 500 hectares. More than 210 varieties of blue honeysuckle of Russian and foreign breeding were collected at the experimental sites of the SPC “Agropishcheprom”. As a result of the breeding work carried out, the Michurinskoye Divo blue honeysuckle variety, which has a set of requirements for industrial varieties, was transferred to the state variety testing. The main problem when laying commercial blue honeysuckle plantations is the lack of high-quality planting material of new varieties. Planting material of blue honeysuckle, on an industrial scale, is obtained in several ways: hardwood cuttings, soft wood cuttings and in vitro method. The work presents the results of studying the influence of planting material obtained by various methods and used when establishing plantations for the growth, development and formation of productivity of the Michurinskoe Divo blue honeysuckle variety. The positive influence of the in vitro propagation method for the qualitative indicators of the root system of the obtained seedlings, growth activity, potential productivity and yield in the production plantings of the Michurinskoe Divo blue honeysuckle variety, in comparison with seedlings obtained by other reproduction methods, was revealed.

show abstract

In vitro propagation of blue honeysuckle

Cited by 15 publications

References 6 publications

In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan

COMPARISON OF PROPAGATION METHOD IN IN VITRO AND IN VIVO CONDITION OF Lonicera caerulea L.

The influence of methods of obtaining planting material on the formation of productivity in blue honeysuckle plantations

Contact Info

Product

Resources

About