In Vitro Propagation of Astragalus Nitidiflorus (Leguminosae), an Endemic and Endangered Species From South-East of Spain

Cano-Castillo, Miriam; Serrano-Martínez, Francisco; Casas, José Luis

doi:10.17660/actahortic.2009.812.79

Cited by 7 publications

(8 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In Spain, several species have been already preserved by means of these techniques (González-Benito et al 1999;González-Benito and Martín 2011), including endemism such as Astragalus nitidiflorus (Cano-Castillo et al 2009) or Helianthemum marminorense (Serrano-Martínez et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…In the search of this objective, we have investigated the use of liquid supplements, or double-phase culture system (DPS), to enhance culture performance as described previously (Cano-Castillo et al 2009;Serrano-Martínez et al 2012). The essential oil content of micropropagated and acclimatized plants was analyzed and compared to that of wild plants in order to ensure that biosynthetic ability was not altered as a consequence of the regeneration protocol.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Marco-Medina

Casas

2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

Thymus moroderi Pau ex Martínez (Lamiaceae) is a species endemic to South-eastern Spain valuable for its use in traditional medicine and in the production of a liqueur called cantueso. We describe a rapid and reliable in vitro propagation protocol for this species, starting from wild adult vegetative explants. Sterilization and establishment of in vitro culture were shown to be extremely difficult because of the high presence of contamination and the inability of non-contaminated explants to restore growth. In just two cases we were able to obtain non-contaminated explants with ability to grow: combining a fungicide dip with the presence of kanamycin in culture medium, and when mother plants were maintained for a week in a growth chamber with occasional fungicide spray. Once established in vitro, explants showed rapid and vigorous growth with spontaneous rooting. The presence of cytokinin alone or in combination with auxin gave worse results than untreated controls, thus revealing Thymus moroderi as a cytokinin-sensitive species. Better multiplication rates were however obtained when double phase system was included in the protocol. Fully acclimatized micropropagated plants were phenotypically indistinguishable from their wild relatives. Essential oils of micropropagated and wild plants were analyzed revealing a slightly different overall terpene profile. Micropropagated plants were abundant in monoterpene and sesquiterpene hydrocarbons but less in oxygenated sesquiterpenes with respect to wild plants. Major compounds were in both cases 1,8-cineole and camphor, although at different relative abundances. Despite these dissimilarities micropropagated plants produced a clearly recognizable and characteristic thyme odor.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

Marco-Medina

Casas

2014

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…(1) Debido a la complejidad de los medios de cultivo, no se describen en el cuadro. Emplazamos al lector a la referencia descrita (2) Aplicación conjunta de medio en doble fase y enfriamiento basal ("bottom cooling") Posteriormente, la composición de la fase líquida fue optimizada en T. articulata (SERRANO-MAR-TíNEZ, 2009) y variaciones de la misma fueron evaluadas también con éxito en Astragalus nitidiflorus (CANO-CASTILLO et al, 2009) En este último caso, además de mejorar la elongación, observamos que gracias a ello, los explantos produjeron un mayor número de nudos, aumentando así la capacidad de multiplicación. Durante los ensayos de multiplicación en H. marminorense, la aplicación de reguladores de crecimiento producía un aumento del número de tallos pero acompañado de un incremento del porcentaje de hiperhidratación y formación de callo basal, dando lugar a tallos adventicios en esta zona.…”

Section: Aplicación De Los Medios En Doble Fase a La Conservación In unclassified

“…Éste podría dar lugar a hiperhidratación debido a la presencia de medio líquido, tal y como observamos en estudios posteriores en T. articulata. En el caso de T. articulata donde también se observó un engrosamiento de la base del explanto con posible aparición de callo si la doble fase se mantenía durante 6-8 semanas seguidas (SERRANO-MARTíNEZ, 2009). Por este motivo, el tiempo de exposición debe también ser tenido en cuenta, así como quizás, la posibilidad de alternar su uso con el cultivo en medio semisólido.…”

Section: Aplicación De Los Medios En Doble Fase a La Conservación In unclassified

El medio en doble fase y su aplicación en el cultivo in vitro de plantas amenazadas

Martínez¹,

Castillo²,

Medina³

2011

cdbio

View full text Add to dashboard Cite

From several decades, in vitro culture techniques are being used as a suitable method for germplasm conservation. The development of micropropagation protocols for rare, endangered, threatened or endemic plant species is sometimes difficult due to the lack of previous in vitro studies. Besides the establishment of in vitro culture of this kind of species, to obtain good multiplication and rooting rates and true-to-type acclimatized plants are main objectives to be achieved with the micropropagation protocols. Problems with the choice of the type and/ or concentration of growth regulators are common in this kind of studies, resulting in callus formation and/or hyperhydricity. According to our experience, the double-phase culture system is a method that can improve growth and/or multiplication rate of in vitro-cultured plants. Since the preparation of the double-phase culture media is easy and the evaluation of the results obtained is fast, experiments to test its effects in the growth and development of in vitro-cultured plants could be of interest to achieve the aim of conservation.

show abstract

“…[20][21][22][23] In some species it is difficult to determine the optimal conditions for sustainable growth, but in vitro micropropagation continues to be a reliable source for conservation of medicinally important endangered plant species. 24 Due to strong antioxidant activity and presence of large amount of biologically active phytochemicals, P. bracteosa may be considered as a potential source for reduction of silver ions (Ag + ) to AgNPs.…”

Section: Introductionmentioning

confidence: 99%

Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa

Anjum

Abbasi

2016

IJN

View full text Add to dashboard Cite

In vitro-derived cultures of plants offer a great potential for rapid biosynthesis of chemical-free antimicrobial silver nanoparticles (AgNPs) by enhancing their phytochemical reducing potential. Here, we developed an efficient protocol for in vitro micropropagation of a high-value endangered medicinal plant species, Phlomis bracteosa , in order to explore its biogenic potential in biomimetic synthesis of antimicrobial AgNPs. Murashige and Skoog medium supplemented with 2.0 mg/L thidiazuron was found to be more efficient in inducing optimum in vitro shoot regeneration (78%±4.09%), and 2.0 mg/L indole-3-butyric acid was used for maximum root induction (86%±4.457%). Antimicrobial AgNPs were successfully synthesized by using aqueous extract (rich in total phenolics and flavonoids content) of in vitro derived plantlets of P. bracteosa . Ultraviolet–visible spectroscopy of synthesized AgNPs showed characteristic surface plasmon band in the range of 420–429 nm. The crystallinity, size, and shape of the AgNPs were characterized by X-ray diffraction and scanning electron microscopy. Face-centered cubic AgNPs of almost uniform spherical size (22.41 nm) were synthesized within a short time (1 hour) at room temperature. Fourier-transform infrared spectroscopy revealed that the polyphenols were mainly responsible for reduction and capping of synthesized AgNPs. Energy dispersive X-ray analysis further endorsed the presence of elemental silver in synthesized AgNPs. These biosynthesized AgNPs displayed significantly higher bactericidal activity against multiple drug-resistant human pathogens. The present work highlighted the potent role of in vitro-derived plantlets of P. bracteosa for feasible biosynthesis of antimicrobial AgNPs, which can be used as nanomedicines in many biomedical applications.

show abstract

In Vitro Propagation of Astragalus Nitidiflorus (Leguminosae), an Endemic and Endangered Species From South-East of Spain

Cited by 7 publications

References 0 publications

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

In vitro multiplication and essential oil composition of Thymus moroderi Pau ex Martinez, an endemic Spanish plant

El medio en doble fase y su aplicación en el cultivo in vitro de plantas amenazadas

Biomimetic synthesis of antimicrobial silver nanoparticles using in vitro-propagated plantlets of a medicinally important endangered species: Phlomis bracteosa

Contact Info

Product

Resources

About